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Image Search Results
Journal: World Journal of Gastroenterology : WJG
Article Title: 4-hydroxy-2, 3-nonenal activates activator protein-1 and mitogen-activated protein kinases in rat pancreatic stellate cells
doi: 10.3748/wjg.v10.i16.2344
Figure Lengend Snippet: PSCs were treated with IL-1β (at 2 ng/mL, lane 2) or HNE (at 5 μmol/L, lane 4) in serum-free medium for 1 h. Nuclear extracts were prepared and subjected to electro-phoretic mobility shift assay using AP-1 (panel A) or NF-κB (panel B) oligonucleotide probes. Arrows denote specific in-ducible complexes competitive with cold double-stranded oli-gonucleotide probes (lane 3). Lane 1: control (serum-free medium only). □: free probe.
Article Snippet: Double-stranded oligonucleotide probes for
Techniques: Mobility Shift
Journal: World Journal of Gastroenterology : WJG
Article Title: 4-hydroxy-2, 3-nonenal activates activator protein-1 and mitogen-activated protein kinases in rat pancreatic stellate cells
doi: 10.3748/wjg.v10.i16.2344
Figure Lengend Snippet: HNE increased AP-1, but not NF-κB, -dependent transcriptional activity. A: PSCs were transfected with the lu-ciferase vectors (2 X AP-1 or 2 X NF-κB) along with pRL-TK vector as an internal control. After 24 h, the transfected cells were treated with IL-1β (at 2 ng/mL) or HNE (at 1 μmol/L ). After another 24-h incubation, intracellular luciferase activities were determined. The data represent mean mean ± SD, calcu-lated from three independent experiments as fold induction compared with the activity observed in control (serum-free medium only, “Con”). bP < 0.01 vs control. RLU: relative light units. B: PSCs were treated with IL-1β (at 2 ng/mL) or HNE (at 1 μmol/L) in serum-free medium for the indicated times. Total cell lysates were prepared, and the level of IκB-α was deter-mined by Western blotting.
Article Snippet: Double-stranded oligonucleotide probes for
Techniques: Activity Assay, Transfection, Plasmid Preparation, Incubation, Luciferase, Western Blot