Journal: PLoS ONE

Article Title: MicroRNA Expression Profiles of Whole Blood in Lung Adenocarcinoma

doi: 10.1371/journal.pone.0046045

Figure Lengend Snippet: The scatter-plots show RT-PCR quantification cycle (C q ) values and log 2 -transformed microarray signal values for microRNAs let-7e , miR-22 , miR-30a-5p , miR-185 , miR-210 , and miR-423-5p (n = 11). Pearson correlation coefficients (r) and their 95% confidence intervals and associated P values, and best fitting (least squares) lines are also shown.

Article Snippet: A two-color, oligonucleotide microarray platform from Exiqon® was used to quantify levels of 1282 human microRNAs and 23 human non-microRNAs of <200 nucleotides in the RNA isolated from whole blood specimens.

Techniques: Reverse Transcription Polymerase Chain Reaction, Transformation Assay, Microarray

Journal: PLoS ONE

Article Title: MicroRNA Expression Profiles of Whole Blood in Lung Adenocarcinoma

doi: 10.1371/journal.pone.0046045

Figure Lengend Snippet: A . Unsupervised clustering of the 45 samples of this study by log 2 -transformed microarray signal values of all 395 expressed microRNAs. The numbers indicate identities of the 45 subjects, with cases (n = 22) and controls (n = 23) shown in black and grey, respectively. The sample tree with optimized leaf-ordering is drawn using Pearson correlation for distance metric and average linkage for cluster-to-cluster distance, and the scale for it represents node-heights. B . Supervised clustering of microRNAs by their log 2 -transformed microarray signal values. The heat-map, with the pseudo-color scale underneath, shows log 2 -transformed microarray signal values of the 43 microRNAs whose expression is altered >25% in either direction in the cases compared to the controls. The gene tree is drawn as in A .

Article Snippet: A two-color, oligonucleotide microarray platform from Exiqon® was used to quantify levels of 1282 human microRNAs and 23 human non-microRNAs of <200 nucleotides in the RNA isolated from whole blood specimens.

Techniques: Transformation Assay, Microarray, Expressing

Journal: PLoS ONE

Article Title: MicroRNA Expression Profiles of Whole Blood in Lung Adenocarcinoma

doi: 10.1371/journal.pone.0046045

Figure Lengend Snippet: Dot-plots with medians and inter-quartile ranges of log 2 -transformed microarray signal values for the 22 cases ( black ) and 23 controls ( grey ) are shown for the four microRNAs that are present in a majority of the classifiers generated in internal cross-validation analyses using the linear support vector machines and top-scoring pairs classification methods.

Article Snippet: A two-color, oligonucleotide microarray platform from Exiqon® was used to quantify levels of 1282 human microRNAs and 23 human non-microRNAs of <200 nucleotides in the RNA isolated from whole blood specimens.

Techniques: Transformation Assay, Microarray, Generated, Plasmid Preparation

Journal: PLoS ONE

Article Title: MicroRNA Expression Profiles of Whole Blood in Lung Adenocarcinoma

doi: 10.1371/journal.pone.0046045

Figure Lengend Snippet: A . Receiver operating characteristic curves, the areas under curve ( AUC ) for age, and the line of identity, x = y , with an AUC of 0.5, are shown. B . Correlation with microRNA expression. Values for the clinical variables were correlated with microarray signal values for the 395 expressed microRNAs (n = 45 for age; n = 39 for others). The curves depict frequency histograms of Pearson correlation coefficients ( r ) with a bin of 0.025. Curves were smoothened using four neighbors for averaging and a zero order polynomial. Correlations are also shown for the random variable resampled WBC count for which values were generated by resampling the WBC count data.

Article Snippet: A two-color, oligonucleotide microarray platform from Exiqon® was used to quantify levels of 1282 human microRNAs and 23 human non-microRNAs of <200 nucleotides in the RNA isolated from whole blood specimens.

Techniques: Expressing, Microarray, Generated

Journal: European Journal of Human Genetics

Article Title: Care and cost consequences of pediatric whole genome sequencing compared to chromosome microarray

doi: 10.1038/s41431-017-0020-3

Figure Lengend Snippet: Number of activities by type prompted by sequential testing scenario: non-diagnostic CMA followed by diagnostic and non-diagnostic WGS results (n = 93). CMA chromosomal microarray analysis, WGS whole genome sequencing. 1. Lab tests may include genetic, biochemical and hematogy tests. Allied health may include audiology, dietetics/nutrition, occupational therapy and physiotherapy, spech and language therapy and psychology. Other activities may include electrocardiogram (ECG) electroencephalogram (EEG), Holter monitor, visual evoked potential (VEP), electromyography (EMG), Electroretinography (ERG), evoked potential, monitoring and surveillance and other. 2. The number of times a given test was implemented was included in the activity count. 3. Activities that are standard after CMA or WGS tests, validation testing (Sanger sequencing) and genetic counselling were not included

Article Snippet: CMA was undertaken using the 4 × 180 K CytoSureISCA v2 oligonucleotide microarray platform (Oxford Gene Technology, Oxford, UK), the standard diagnostic service offered by SickKids Clinical Laboratory Improvement Amendments (CLIA)-certified diagnostic laboratory.

Techniques: Diagnostic Assay, Microarray, Sequencing, Activity Assay, Biomarker Discovery