Journal: Cellular and Molecular Immunology

Article Title: Naturally occurring spike mutations influence the infectivity and immunogenicity of SARS-CoV-2

doi: 10.1038/s41423-022-00924-8

Figure Lengend Snippet: Substitution of S982A and L18F weakens the generation of binding antibodies. The area under the curve (AUC) of anti-spike ( A ) and anti-RBD ( B ) IgG induced by the 19 designed groups of DNA immunogens ( n = 3 for each group). The AUC represents the total peak area calculated from ELISA OD values by GraphPad Prism v8. Data were analyzed for statistical significance using the unpaired Student’s t test. Data are shown as the mean with SD in each group. Dotted black lines indicate the limit of quantification (LOQ). * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: The AUC represents the total peak area calculated from ELISA OD values by GraphPad Prism v8.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: EBioMedicine

Article Title: Waning immune responses against SARS-CoV-2 variants of concern among vaccinees in Hong Kong

doi: 10.1016/j.ebiom.2022.103904

Figure Lengend Snippet: Immune responses measured at the acute phase after the second dose among BNT162b2 and CoronaVac vaccinees. ( a ) The area under curve (AUC) of anti-Spike and anti-RBD IgG in BNT162b2 (orange) (n=34), CoronaVac (green) (n=28) and non-vaccinated volunteers (grey) (n=16). The AUC represents the total peak area calculated from ELISA OD values by the GraphPad Prism v8. ( b ) Percentage inhibition and IC 50 /IC 90 values against wild type SARS-CoV-2 pseudoviruses in BNT162b2 and CoronaVac vaccinees. ( c ) The neutralization antibody potency index defined by the ratio of IC 50 /AUC of anti-Spike IgG and anti-RBD IgG in BNT162b2 and CoronaVac group. Data showed geometric mean values in each group in a - c . ( d ) Quantified results depict the percentage of RBD, spike and NP-specific IFN-γ + CD4 + T (top) and IFN-γ + CD8 + T (bottom) cells in BNT162b2 (n=33), CoronaVac (n=28) and non-vaccinated volunteers (n=15), respectively. Fresh PBMC were subjected to T cell response measurement by ICS after RBD-, spike- and NP-specific ex vivo peptide pool stimulation, respectively. Numbers under the x-axis in a and d indicate the rate of positive responders. ( e ) The proportions of spike-specific polyfunctional CD4 + T (top) and CD8 + T (bottom) cells were compared in BNT162b2 and CoronaVac-vaccinated responders. After gating on IFN-γ + CD4 + /CD8 + T cells and IFN-γ − CD4 + /CD8 + T cells, single cytokine (IFN-γ + or TNF-α + or IL-2 + only), double cytokines (IFN-γ + TNF-α + or IFN-γ + IL-2 + or TNF-α + IL-2 + ), and triple cytokines (IFN-γ + TNF-α + IL-2 + ) producing cells were analyzed in response to spike-specific ex vivo peptide pool stimulation, respectively. Background-subtracted data was analyzed in all cases in d and e . The bars in D and E indicated median value. ( f ) Phenotypic analysis depicted antigen-specific T cell subsets of BNT162b2 and CoronaVac vaccinees. After gating on IFN-γ + CD4 + or IFN-γ + CD8 + T cells, T cell subsets expressing CCR7 and/or CD45RA were analyzed in response to spike-specific ex vivo peptide pool stimulation. Data were analyzed for statistical significance using Mann-Whitney U test. Dotted black lines indicate the limit of quantification (LOQ). ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The AUC represents the total peak area calculated from ELISA OD values by the GraphPad Prism v8. ( b ) Percentage inhibition and IC 50 /IC 90 values against wild type SARS-CoV-2 pseudoviruses in BNT162b2 and CoronaVac vaccinees. ( c ) The neutralization antibody potency index defined by the ratio of IC 50 /AUC of anti-Spike IgG and anti-RBD IgG in BNT162b2 and CoronaVac group.

Techniques: Enzyme-linked Immunosorbent Assay, Inhibition, Neutralization, Ex Vivo, Expressing, MANN-WHITNEY