Journal: Journal of Histochemistry and Cytochemistry
Article Title: Identification of Specific Chondroitin Sulfate Species in Cutaneous Autoimmune Disease
Figure Lengend Snippet: Cutaneous lupus and dermatomyositis lesions express increased chondroitin-4-sulfate (C4S) in the dermal matrix. Formalin-fixed, paraffin-embedded skin sections of (a) healthy control skin, (b) DLE lesional skin, (c) SCLE lesional skin, and (d, e) DM lesional
Article Snippet: Immunostaining of chondroitin-6-sulfate and chondroitin-4-sulfate was achieved by predigestion with chondroitinase AC II enzyme (Sigma, St. Louis, MO) followed by anti-proteoglycan ΔDi-6S or anti-proteoglycan ΔDi-4S monoclonal antibody; these antibodies recognize a terminal glucuronic acid residue adjacent to N-acetylgalactosamine-6- or 4-sulphate at the non-reducing end of chondroitin sulfate chains in proteoglycans produced after chondroitinase digestion of chondroitin sulphate chains (clones 2-B-6 and 3-B-3, Seikagaku Biobusiness Corporation, Tokyo, Japan).
Techniques: Formalin-fixed Paraffin-Embedded