octasaccharides Search Results


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  • 95
    Millipore hexasaccharide
    ( A ) Binding preferences of mIL-7 to various chain lengths of heparin using a SPR competition assay. Heparin disaccharide (dp2), tetrasaccharide (dp4), <t>hexasaccharide</t> (dp6), and octasaccharide (dp8) were each pre-equilibrated at a concentration of 1,000
    Hexasaccharide, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore tetrasaccharide
    ( A ) Binding preferences of mIL-7 to various chain lengths of heparin using a SPR competition assay. Heparin disaccharide (dp2), tetrasaccharide (dp4), <t>hexasaccharide</t> (dp6), and octasaccharide (dp8) were each pre-equilibrated at a concentration of 1,000
    Tetrasaccharide, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 20 article reviews
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    99
    Millipore chondroitin 4 sulfate
    <t>Chondroitin-4-sulfate</t> is significantly increased in scleredema and scleromyxedema lesions. Formalin-fixed, paraffin-embedded skin sections of (a) healthy control skin, (b) scleredema, and (c) scleromyxedema were stained with an antibody specific for chondroitin-4-sulfate
    Chondroitin 4 Sulfate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Mimetics heparin mimetics
    <t>Chondroitin-4-sulfate</t> is significantly increased in scleredema and scleromyxedema lesions. Formalin-fixed, paraffin-embedded skin sections of (a) healthy control skin, (b) scleredema, and (c) scleromyxedema were stained with an antibody specific for chondroitin-4-sulfate
    Heparin Mimetics, supplied by Mimetics, used in various techniques. Bioz Stars score: 91/100, based on 534 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Medicago rhizobium meliloti
    Western blot showing ExoR protein profiles of the S. <t>meliloti</t> wild-type Rm1021, the exoR95 mutant, the exoR108-chvI109 double mutant, and the exoR108-chvI109 (pHC510) double mutant, which is labeled as a p exoR mutant expressing the wild-type exoR gene.
    Rhizobium Meliloti, supplied by Medicago, used in various techniques. Bioz Stars score: 91/100, based on 233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Biacore heparan sulfate
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Heparan Sulfate, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher ltq orbitrap xl instrument
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Ltq Orbitrap Xl Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 551 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Malvern Panalytical malvern peaq itc microcalorimeter
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Malvern Peaq Itc Microcalorimeter, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Mimetics sulfated heparin mimetics hm
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Sulfated Heparin Mimetics Hm, supplied by Mimetics, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avanti Polar 1 2 dioleoyl sn glycero 3 phosphoethanolamine dope
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    1 2 Dioleoyl Sn Glycero 3 Phosphoethanolamine Dope, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 92/100, based on 570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher 500 ion chromatography system
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    500 Ion Chromatography System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Bruker Corporation 500 mhz bruker avance nmr spectrometer
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    500 Mhz Bruker Avance Nmr Spectrometer, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 88/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Chemical Computing Group amber99 force field
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Amber99 Force Field, supplied by Chemical Computing Group, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Cell Signaling Technology Inc anti akt antibody 9272 antibodies
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Anti Akt Antibody 9272 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2 article reviews
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    92
    Cell Signaling Technology Inc anti phospho erk1 2
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Anti Phospho Erk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 4944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad anti human monoclonal antibodies
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Anti Human Monoclonal Antibodies, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Santa Cruz Biotechnology anti human polyclonal antibody
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Anti Human Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Brookhaven Instruments national synchrotron light source
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    National Synchrotron Light Source, supplied by Brookhaven Instruments, used in various techniques. Bioz Stars score: 94/100, based on 2876 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore cnbr activated sepharose 4b
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Cnbr Activated Sepharose 4b, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Mimetics recombinant sdcs
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Recombinant Sdcs, supplied by Mimetics, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Nacalai rp hplc
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Rp Hplc, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Seikagaku chondroitin sulfate d
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Chondroitin Sulfate D, supplied by Seikagaku, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Megazyme tamarind xyloglucan
    27 Al-NMR Profile of AlCl 3 in the Presence of Citrate and <t>Xyloglucan.</t>
    Tamarind Xyloglucan, supplied by Megazyme, used in various techniques. Bioz Stars score: 91/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore bovine kidney
    27 Al-NMR Profile of AlCl 3 in the Presence of Citrate and <t>Xyloglucan.</t>
    Bovine Kidney, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) Binding preferences of mIL-7 to various chain lengths of heparin using a SPR competition assay. Heparin disaccharide (dp2), tetrasaccharide (dp4), hexasaccharide (dp6), and octasaccharide (dp8) were each pre-equilibrated at a concentration of 1,000

    Journal: Biochimie

    Article Title: Biophysical Characterization of Glycosaminoglycan-IL-7 Interactions using SPR

    doi: 10.1016/j.biochi.2011.10.015

    Figure Lengend Snippet: ( A ) Binding preferences of mIL-7 to various chain lengths of heparin using a SPR competition assay. Heparin disaccharide (dp2), tetrasaccharide (dp4), hexasaccharide (dp6), and octasaccharide (dp8) were each pre-equilibrated at a concentration of 1,000

    Article Snippet: A disaccharide (dp2), tetrasaccharide (dp4), hexasaccharide (dp6), and octasaccharide (dp8) form of heparin was prepared from controlled partial heparin lyase 1 treatment of bovine lung heparin (Sigma) [ ].

    Techniques: Binding Assay, SPR Assay, Competitive Binding Assay, Concentration Assay

    ( A ) Binding preferences of human IL-7 to various chain lengths of heparin using a SPR competition assay. A disaccharide (dp2), a tetrasaccharide (dp4), a hexasaccharide (dp6), and an octasaccarharide (dp8) of heparin was pre-equilibrated at a concentration

    Journal: Biochimie

    Article Title: Biophysical Characterization of Glycosaminoglycan-IL-7 Interactions using SPR

    doi: 10.1016/j.biochi.2011.10.015

    Figure Lengend Snippet: ( A ) Binding preferences of human IL-7 to various chain lengths of heparin using a SPR competition assay. A disaccharide (dp2), a tetrasaccharide (dp4), a hexasaccharide (dp6), and an octasaccarharide (dp8) of heparin was pre-equilibrated at a concentration

    Article Snippet: A disaccharide (dp2), tetrasaccharide (dp4), hexasaccharide (dp6), and octasaccharide (dp8) form of heparin was prepared from controlled partial heparin lyase 1 treatment of bovine lung heparin (Sigma) [ ].

    Techniques: Binding Assay, SPR Assay, Competitive Binding Assay, Concentration Assay

    Chondroitin-4-sulfate is significantly increased in scleredema and scleromyxedema lesions. Formalin-fixed, paraffin-embedded skin sections of (a) healthy control skin, (b) scleredema, and (c) scleromyxedema were stained with an antibody specific for chondroitin-4-sulfate

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Identification of Specific Chondroitin Sulfate Species in Cutaneous Autoimmune Disease

    doi: 10.1369/0022155411411304

    Figure Lengend Snippet: Chondroitin-4-sulfate is significantly increased in scleredema and scleromyxedema lesions. Formalin-fixed, paraffin-embedded skin sections of (a) healthy control skin, (b) scleredema, and (c) scleromyxedema were stained with an antibody specific for chondroitin-4-sulfate

    Article Snippet: Immunostaining of chondroitin-6-sulfate and chondroitin-4-sulfate was achieved by predigestion with chondroitinase AC II enzyme (Sigma, St. Louis, MO) followed by anti-proteoglycan ΔDi-6S or anti-proteoglycan ΔDi-4S monoclonal antibody; these antibodies recognize a terminal glucuronic acid residue adjacent to N-acetylgalactosamine-6- or 4-sulphate at the non-reducing end of chondroitin sulfate chains in proteoglycans produced after chondroitinase digestion of chondroitin sulphate chains (clones 2-B-6 and 3-B-3, Seikagaku Biobusiness Corporation, Tokyo, Japan).

    Techniques: Formalin-fixed Paraffin-Embedded, Staining

    Cutaneous lupus and dermatomyositis lesions express increased chondroitin-4-sulfate (C4S) in the dermal matrix. Formalin-fixed, paraffin-embedded skin sections of (a) healthy control skin, (b) DLE lesional skin, (c) SCLE lesional skin, and (d, e) DM lesional

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: Identification of Specific Chondroitin Sulfate Species in Cutaneous Autoimmune Disease

    doi: 10.1369/0022155411411304

    Figure Lengend Snippet: Cutaneous lupus and dermatomyositis lesions express increased chondroitin-4-sulfate (C4S) in the dermal matrix. Formalin-fixed, paraffin-embedded skin sections of (a) healthy control skin, (b) DLE lesional skin, (c) SCLE lesional skin, and (d, e) DM lesional

    Article Snippet: Immunostaining of chondroitin-6-sulfate and chondroitin-4-sulfate was achieved by predigestion with chondroitinase AC II enzyme (Sigma, St. Louis, MO) followed by anti-proteoglycan ΔDi-6S or anti-proteoglycan ΔDi-4S monoclonal antibody; these antibodies recognize a terminal glucuronic acid residue adjacent to N-acetylgalactosamine-6- or 4-sulphate at the non-reducing end of chondroitin sulfate chains in proteoglycans produced after chondroitinase digestion of chondroitin sulphate chains (clones 2-B-6 and 3-B-3, Seikagaku Biobusiness Corporation, Tokyo, Japan).

    Techniques: Formalin-fixed Paraffin-Embedded

    Western blot showing ExoR protein profiles of the S. meliloti wild-type Rm1021, the exoR95 mutant, the exoR108-chvI109 double mutant, and the exoR108-chvI109 (pHC510) double mutant, which is labeled as a p exoR mutant expressing the wild-type exoR gene.

    Journal: Journal of Bacteriology

    Article Title: Sinorhizobium meliloti ExoR Is the Target of Periplasmic Proteolysis

    doi: 10.1128/JB.00313-12

    Figure Lengend Snippet: Western blot showing ExoR protein profiles of the S. meliloti wild-type Rm1021, the exoR95 mutant, the exoR108-chvI109 double mutant, and the exoR108-chvI109 (pHC510) double mutant, which is labeled as a p exoR mutant expressing the wild-type exoR gene.

    Article Snippet: Biosynthetic control of molecular weight in the polymerization of the octasaccharide subunits of succinoglycan, a symbiotically important exopolysaccharide of Rhizobium meliloti .

    Techniques: Western Blot, Mutagenesis, Labeling, Expressing

    Cellular location of ExoR protein and site of proteolysis. (A and B) Schematic representation of three different ExoR-PhoA fusions (A) and their corresponding phosphatase activities in the S. meliloti Rm8002 background (B). The vertically hatched boxes

    Journal: Journal of Bacteriology

    Article Title: Sinorhizobium meliloti ExoR Is the Target of Periplasmic Proteolysis

    doi: 10.1128/JB.00313-12

    Figure Lengend Snippet: Cellular location of ExoR protein and site of proteolysis. (A and B) Schematic representation of three different ExoR-PhoA fusions (A) and their corresponding phosphatase activities in the S. meliloti Rm8002 background (B). The vertically hatched boxes

    Article Snippet: Biosynthetic control of molecular weight in the polymerization of the octasaccharide subunits of succinoglycan, a symbiotically important exopolysaccharide of Rhizobium meliloti .

    Techniques:

    Amino acid sequence alignment of ExoR proteolysis regions from 16 ExoR orthologs. Conserved amino acids are shaded. The overall amino acid identities between S. meliloti ExoR and its orthologs are listed. The positions of the amino acids and the site

    Journal: Journal of Bacteriology

    Article Title: Sinorhizobium meliloti ExoR Is the Target of Periplasmic Proteolysis

    doi: 10.1128/JB.00313-12

    Figure Lengend Snippet: Amino acid sequence alignment of ExoR proteolysis regions from 16 ExoR orthologs. Conserved amino acids are shaded. The overall amino acid identities between S. meliloti ExoR and its orthologs are listed. The positions of the amino acids and the site

    Article Snippet: Biosynthetic control of molecular weight in the polymerization of the octasaccharide subunits of succinoglycan, a symbiotically important exopolysaccharide of Rhizobium meliloti .

    Techniques: Sequencing

    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

    Journal:

    Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

    doi: 10.1074/jbc.M109.002840

    Figure Lengend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

    Article Snippet: The inhibition of α5β1 integrin binding to heparin and heparan sulfate increased with the length of oligosaccharides and then reached a plateau.

    Techniques: SPR Assay, Binding Assay, Injection

    Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

    Journal:

    Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

    doi: 10.1074/jbc.M109.002840

    Figure Lengend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

    Article Snippet: The inhibition of α5β1 integrin binding to heparin and heparan sulfate increased with the length of oligosaccharides and then reached a plateau.

    Techniques:

    27 Al-NMR Profile of AlCl 3 in the Presence of Citrate and Xyloglucan.

    Journal: The Plant Cell

    Article Title: XTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in Arabidopsis [W]

    doi: 10.1105/tpc.112.106039

    Figure Lengend Snippet: 27 Al-NMR Profile of AlCl 3 in the Presence of Citrate and Xyloglucan.

    Article Snippet: The supernatant enzyme solution (protein concentration 0.12 to 0.69 µg/μL by the Bradford assay) was aliquoted and stored at –80°C. activity was assayed in a reaction mixture containing the buffered enzyme solution (64% v/v), 11.6 Bq/μL [ reducing-end -3 H]xyloglucan, and 0.18% (w/v) chlorobutanol at 20°C for 12 and 24 h. The [3 H]octasaccharide released by hydrolysis (from 232 Bq substrate) was assayed by paper chromatography as above. activity was assayed in a reaction mixture containing buffered enzyme solution (50%, v/v), 2.5 µg/μL tamarind xyloglucan, and 114 Bq/μL [1-3 H]XXLGol at 20°C.

    Techniques: Nuclear Magnetic Resonance

    Analysis of the Relative Abundance of Xyloglucan Oligosaccharides Released by Xyloglucanase.

    Journal: The Plant Cell

    Article Title: XTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in Arabidopsis [W]

    doi: 10.1105/tpc.112.106039

    Figure Lengend Snippet: Analysis of the Relative Abundance of Xyloglucan Oligosaccharides Released by Xyloglucanase.

    Article Snippet: The supernatant enzyme solution (protein concentration 0.12 to 0.69 µg/μL by the Bradford assay) was aliquoted and stored at –80°C. activity was assayed in a reaction mixture containing the buffered enzyme solution (64% v/v), 11.6 Bq/μL [ reducing-end -3 H]xyloglucan, and 0.18% (w/v) chlorobutanol at 20°C for 12 and 24 h. The [3 H]octasaccharide released by hydrolysis (from 232 Bq substrate) was assayed by paper chromatography as above. activity was assayed in a reaction mixture containing buffered enzyme solution (50%, v/v), 2.5 µg/μL tamarind xyloglucan, and 114 Bq/μL [1-3 H]XXLGol at 20°C.

    Techniques: