oas1 Search Results


gene exp oas1 hs00973635 m1  (Thermo Fisher)
98
Thermo Fisher gene exp oas1 hs00973635 m1
Gene Exp Oas1 Hs00973635 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti oas1 antibody  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc rabbit anti oas1 antibody
IFN response in pathogenic-arenavirus-infected PKR-deficient cells. wt A549 cells and PKR-deficient A549 cells were mock infected or infected with pathogenic NW JUNV, MACV, or OW LASV at an MOI of 3. (A) Real-time RT-qPCR analysis was performed to quantitate IFN-β mRNA at 24 hpi. Data were normalized to the levels of the human housekeeping β-actin gene and are presented as the means ± SEM from three experiments. (B) Western blotting data for ISG proteins <t>(OAS1,</t> ISG15, and RIG-I) and human β-actin at 24 hpi.
Rabbit Anti Oas1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti oas1 rabbit polyclonal 14955 1 ap  (Proteintech)
93
Proteintech anti oas1 rabbit polyclonal 14955 1 ap
IFN response in pathogenic-arenavirus-infected PKR-deficient cells. wt A549 cells and PKR-deficient A549 cells were mock infected or infected with pathogenic NW JUNV, MACV, or OW LASV at an MOI of 3. (A) Real-time RT-qPCR analysis was performed to quantitate IFN-β mRNA at 24 hpi. Data were normalized to the levels of the human housekeeping β-actin gene and are presented as the means ± SEM from three experiments. (B) Western blotting data for ISG proteins <t>(OAS1,</t> ISG15, and RIG-I) and human β-actin at 24 hpi.
Anti Oas1 Rabbit Polyclonal 14955 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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oas 1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology oas 1
IFN response in pathogenic-arenavirus-infected PKR-deficient cells. wt A549 cells and PKR-deficient A549 cells were mock infected or infected with pathogenic NW JUNV, MACV, or OW LASV at an MOI of 3. (A) Real-time RT-qPCR analysis was performed to quantitate IFN-β mRNA at 24 hpi. Data were normalized to the levels of the human housekeeping β-actin gene and are presented as the means ± SEM from three experiments. (B) Western blotting data for ISG proteins <t>(OAS1,</t> ISG15, and RIG-I) and human β-actin at 24 hpi.
Oas 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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mr220810 mouse oas1g  (OriGene)
92
OriGene mr220810 mouse oas1g
IFN response in pathogenic-arenavirus-infected PKR-deficient cells. wt A549 cells and PKR-deficient A549 cells were mock infected or infected with pathogenic NW JUNV, MACV, or OW LASV at an MOI of 3. (A) Real-time RT-qPCR analysis was performed to quantitate IFN-β mRNA at 24 hpi. Data were normalized to the levels of the human housekeeping β-actin gene and are presented as the means ± SEM from three experiments. (B) Western blotting data for ISG proteins <t>(OAS1,</t> ISG15, and RIG-I) and human β-actin at 24 hpi.
Mr220810 Mouse Oas1g, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal  (ABclonal Biotechnology)
94
ABclonal Biotechnology rabbit polyclonal
IFN response in pathogenic-arenavirus-infected PKR-deficient cells. wt A549 cells and PKR-deficient A549 cells were mock infected or infected with pathogenic NW JUNV, MACV, or OW LASV at an MOI of 3. (A) Real-time RT-qPCR analysis was performed to quantitate IFN-β mRNA at 24 hpi. Data were normalized to the levels of the human housekeeping β-actin gene and are presented as the means ± SEM from three experiments. (B) Western blotting data for ISG proteins <t>(OAS1,</t> ISG15, and RIG-I) and human β-actin at 24 hpi.
Rabbit Polyclonal, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oas1 transcript variant 2 cdna  (OriGene)
90
OriGene oas1 transcript variant 2 cdna
Figure 7. Idarubicin induces STAT1-dependent gene expression. U3A-STAT1 and U3A (STAT1-null) cells were treated with idarubicin for 1.5 h followed by IFN-b for 12 h and then determination of <t>OAS1</t> and GBP1 mRNA levels using real-time quantitative PCR assay. Overall significance for idarubicin dose p,0.0001, IFN-b dose p,0.0001, and interaction p,0.0001. The p-values for individual comparisons (vs 0 idarubicin control) are from Bonferroni post-tests from two-way ANOVA (idarubicin x IFN dose). Symbols: * p,0.01, ** p,0.001. doi:10.1371/journal.pone.0036594.g007
Oas1 Transcript Variant 2 Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plx302 oas1 v5 puro  (Addgene inc)
93
Addgene inc plx302 oas1 v5 puro
Figure 7. Idarubicin induces STAT1-dependent gene expression. U3A-STAT1 and U3A (STAT1-null) cells were treated with idarubicin for 1.5 h followed by IFN-b for 12 h and then determination of <t>OAS1</t> and GBP1 mRNA levels using real-time quantitative PCR assay. Overall significance for idarubicin dose p,0.0001, IFN-b dose p,0.0001, and interaction p,0.0001. The p-values for individual comparisons (vs 0 idarubicin control) are from Bonferroni post-tests from two-way ANOVA (idarubicin x IFN dose). Symbols: * p,0.01, ** p,0.001. doi:10.1371/journal.pone.0036594.g007
Plx302 Oas1 V5 Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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oas1  (OriGene)
90
OriGene oas1
Figure 7. Idarubicin induces STAT1-dependent gene expression. U3A-STAT1 and U3A (STAT1-null) cells were treated with idarubicin for 1.5 h followed by IFN-b for 12 h and then determination of <t>OAS1</t> and GBP1 mRNA levels using real-time quantitative PCR assay. Overall significance for idarubicin dose p,0.0001, IFN-b dose p,0.0001, and interaction p,0.0001. The p-values for individual comparisons (vs 0 idarubicin control) are from Bonferroni post-tests from two-way ANOVA (idarubicin x IFN dose). Symbols: * p,0.01, ** p,0.001. doi:10.1371/journal.pone.0036594.g007
Oas1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna  (OriGene)
91
OriGene rna
Figure 7. Idarubicin induces STAT1-dependent gene expression. U3A-STAT1 and U3A (STAT1-null) cells were treated with idarubicin for 1.5 h followed by IFN-b for 12 h and then determination of <t>OAS1</t> and GBP1 mRNA levels using real-time quantitative PCR assay. Overall significance for idarubicin dose p,0.0001, IFN-b dose p,0.0001, and interaction p,0.0001. The p-values for individual comparisons (vs 0 idarubicin control) are from Bonferroni post-tests from two-way ANOVA (idarubicin x IFN dose). Symbols: * p,0.01, ** p,0.001. doi:10.1371/journal.pone.0036594.g007
Rna, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti oas1 polyclonal antibody  (Novus Biologicals)
90
Novus Biologicals anti oas1 polyclonal antibody
Figure 7. Idarubicin induces STAT1-dependent gene expression. U3A-STAT1 and U3A (STAT1-null) cells were treated with idarubicin for 1.5 h followed by IFN-b for 12 h and then determination of <t>OAS1</t> and GBP1 mRNA levels using real-time quantitative PCR assay. Overall significance for idarubicin dose p,0.0001, IFN-b dose p,0.0001, and interaction p,0.0001. The p-values for individual comparisons (vs 0 idarubicin control) are from Bonferroni post-tests from two-way ANOVA (idarubicin x IFN dose). Symbols: * p,0.01, ** p,0.001. doi:10.1371/journal.pone.0036594.g007
Anti Oas1 Polyclonal Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


IFN response in pathogenic-arenavirus-infected PKR-deficient cells. wt A549 cells and PKR-deficient A549 cells were mock infected or infected with pathogenic NW JUNV, MACV, or OW LASV at an MOI of 3. (A) Real-time RT-qPCR analysis was performed to quantitate IFN-β mRNA at 24 hpi. Data were normalized to the levels of the human housekeeping β-actin gene and are presented as the means ± SEM from three experiments. (B) Western blotting data for ISG proteins (OAS1, ISG15, and RIG-I) and human β-actin at 24 hpi.

Journal: Journal of Virology

Article Title: Highly Pathogenic New World Arenavirus Infection Activates the Pattern Recognition Receptor Protein Kinase R without Attenuating Virus Replication in Human Cells

doi: 10.1128/JVI.01090-17

Figure Lengend Snippet: IFN response in pathogenic-arenavirus-infected PKR-deficient cells. wt A549 cells and PKR-deficient A549 cells were mock infected or infected with pathogenic NW JUNV, MACV, or OW LASV at an MOI of 3. (A) Real-time RT-qPCR analysis was performed to quantitate IFN-β mRNA at 24 hpi. Data were normalized to the levels of the human housekeeping β-actin gene and are presented as the means ± SEM from three experiments. (B) Western blotting data for ISG proteins (OAS1, ISG15, and RIG-I) and human β-actin at 24 hpi.

Article Snippet: Primary antibodies used for WB analysis were monoclonal antipuromycin antibody (clone 12D10; Millipore), rabbit anti-PKR antibody (number 3072S; Cell Signaling), rabbit anti-PKR phosphor (T451) antibody (ab81303; Abcam), rabbit anti-phospho-eIF2α antibody Ser51 (number 9721S; Cell Signaling), rabbit anti-eIF2α antibody (number 9722S; Cell Signaling), rabbit anti-ISG15 antibody (number 2743; Cell Signaling), rabbit anti-RIGI antibody (number 3743; Cell Signaling), rabbit anti-OAS1 antibody (number 14498; Cell Signaling), and goat anti-human β-actin antibody (sc-1616; Santa Cruz Biotechnology).

Techniques: Infection, Quantitative RT-PCR, Western Blot

Figure 7. Idarubicin induces STAT1-dependent gene expression. U3A-STAT1 and U3A (STAT1-null) cells were treated with idarubicin for 1.5 h followed by IFN-b for 12 h and then determination of OAS1 and GBP1 mRNA levels using real-time quantitative PCR assay. Overall significance for idarubicin dose p,0.0001, IFN-b dose p,0.0001, and interaction p,0.0001. The p-values for individual comparisons (vs 0 idarubicin control) are from Bonferroni post-tests from two-way ANOVA (idarubicin x IFN dose). Symbols: * p,0.01, ** p,0.001. doi:10.1371/journal.pone.0036594.g007

Journal: PloS one

Article Title: High throughput screening for small molecule enhancers of the interferon signaling pathway to drive next-generation antiviral drug discovery.

doi: 10.1371/journal.pone.0036594

Figure Lengend Snippet: Figure 7. Idarubicin induces STAT1-dependent gene expression. U3A-STAT1 and U3A (STAT1-null) cells were treated with idarubicin for 1.5 h followed by IFN-b for 12 h and then determination of OAS1 and GBP1 mRNA levels using real-time quantitative PCR assay. Overall significance for idarubicin dose p,0.0001, IFN-b dose p,0.0001, and interaction p,0.0001. The p-values for individual comparisons (vs 0 idarubicin control) are from Bonferroni post-tests from two-way ANOVA (idarubicin x IFN dose). Symbols: * p,0.01, ** p,0.001. doi:10.1371/journal.pone.0036594.g007

Article Snippet: A plasmid containing OAS1 transcript variant 2 cDNA (Ref ID NM_002534, Origene, Rockville, MD) was used as a standard for absolute quantitation of OAS1 copy number.

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Control