Journal: PLoS ONE
Article Title: Antineuropathic Profile of N-Palmitoylethanolamine in a Rat Model of Oxaliplatin-Induced Neurotoxicity
Figure Lengend Snippet: Inflammation-related mediators. On day 21, protein expression levels of IκBα were quantified by immunoblot in a) DRG and b) spinal cord; c) protein expression levels of COX2 were quantified by immunoblot in spinal cord Animals were treated daily i.p. with 2.4 mg kg -1 oxaliplatin or vehicle for 21 days. PEA (30 mg kg -1 ) was repeatedly administered i.p. (daily for 20 days starting from the first day of oxaliplatin administration). Control animals were treated with vehicles. Densitometric analysis is shown. β-actin normalization was performed for each sample . Each value represents the mean of 5 rats per group, performed in two different experimental sets. ^P
Article Snippet: HT-29 were cultured in DMEM high glucose with 20% FBS in 5% CO2 atmosphere at 37° C. Media contained 2 mM L-glutamine, 1% essential aminoacid mix, 100 IU ml-1 penicillin and 100 μg ml-1 streptomycin (Sigma, Milan, Italy).