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Image Search Results
Journal: PLoS ONE
Article Title: Synthesis of UDP-apiose in Bacteria: The marine phototroph Geminicoccus roseus and the plant pathogen Xanthomonas pisi
doi: 10.1371/journal.pone.0184953
Figure Lengend Snippet: Analysis of in microbe nucleotide sugars by HILIC-LC-ESI-MS/MS. (A) top panel elution of standard (Std): UDP-GlcA, UDP-Xyl and UDP-arabinopyranose (UDP-Ara p ); Nucleotide sugars were extracted from E . coli cells induced to express genes encoding CeUAS, GrUAS, XpUAS or empty vector as the control (bottom panel). [M-H] - ions diagnostic for UDP-pentose ( m/z 535.0, solid line), UDP-hexuronic acid ( m/z 579.0, dashed line) and Park’s nucleotide ( m/z 595.6, dotted line) are displayed. Park’s nucleotide is a UDP-MurNAc-pentapeptide that is used as an internal standard for nucleotide-sugar detection as it is abundantly made in E . coli . The m/z signal for CeUAS and XpUAS is amplified by a factor of 10. (B) Second stage MS fragmentation data for the peaks at the indicated retention times; Left column 11.0 min and right column 12.0 min. MS/MS ions at m/z 323.0, 211.0, 403.0 are consistent with predicted fragmentation of a UDP-sugar into [UMP-H] - , [Ura-2H] - , and [UDP-H 2 O-H] - , respectively.
Article Snippet: Because no axenic monoculture of C . entotheonella was available, a synthetic ORF gene corresponding to the nucleotide sequence of
Techniques: Hydrophilic Interaction Liquid Chromatography, Tandem Mass Spectroscopy, Plasmid Preparation, Control, Diagnostic Assay, Amplification