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Image Search Results
Journal: PLoS ONE
Article Title: Anti-Tumor and Immune Enhancing Activities of Rice Bran Gramisterol on Acute Myelogenous Leukemia
doi: 10.1371/journal.pone.0146869
Figure Lengend Snippet: (A), (B) MTT assay of WEHI-3 cells treated with different concentrations of RBDS for 24, 48 and 72 h, and gramisterol for 24 h. Percent cell viability was calculated relative to the untreated control. The experiments were performed in triplicate and data are presented as mean±SEM. Significant difference was considered at p <0.05 compared with the control. (C) Phase contrast photographs and DAPI-DNA staining of WEHI-3 cells after treatment with RBDS 0, 50, 100 and 200 μg/mL for 48 h. The apoptotic bodies are indicated by white arrowheads. (D) Gel electrophoresis of DNA extracted from RBDS treated-WEHI-3 for 48 h. DNA ladder or fragmentation indicated apoptosis event in the cells. (E) Flow cytometry analysis of WEHI-3 cells after 48 h RBDS-treatment and annexin-V staining. Percent cell count of apoptosis detection was demonstrated from the average of two experiments.
Article Snippet: The treated cells were cold fixed with methanol, air dried and stained with
Techniques: MTT Assay, Control, Staining, Nucleic Acid Electrophoresis, Flow Cytometry, Cell Counting
Journal: Oncology Letters
Article Title: Application of a novel liquid biopsy in patients with hepatocellular carcinoma undergoing liver transplantation
doi: 10.3892/ol.2018.8019
Figure Lengend Snippet: Identification of CTCs in HCC patients using iFISH platform. CK: Green, CEP8: Orange, DAPI: Blue, CD45: Red. (A) CK+/CD45-/DAPI+/CEP8=2; (B) CK+/CD45-/DAPI+/CEP8>2; (C) CK-/CD45+/DAPI+/CEP8>2. (D) CTC cluster. (E) CK-/CD45+/DAPI+/CEP8=2, WBC. iFISH, immunostaining-fluorescence in situ hybridization; CTCs, circulating tumor cells; HCC, hepatocellular carcinoma; CK, cytokeratin; CEP8, centromere probe 8; CD, cluster of differentiation; WBC, white blood cell.
Article Snippet: Immunofluorescence reagent (cat no. 7900001;
Techniques: Immunostaining, Fluorescence, In Situ Hybridization