Journal: Nucleic Acids Research

Article Title: Enzymatic synthesis of random sequences of RNA and RNA analogues by DNA polymerase theta mutants for the generation of aptamer libraries

doi: 10.1093/nar/gky413

Figure Lengend Snippet: Incorporation of modified nucleotides by pol θ WT in comparison with the pol θ CS13. The following nucleotide analogs were tested for the elongation of a ssDNA primer by pol θ CS13 compared to pol θ ( 1 ) 2′-amino-dATP, ( 2 ) 2′-amino-dUTP, ( 3 ) 2′-amino-dCTP, ( 4 ) 2′-amino-dGTP, ( 5 ) mix of 2′-amino-dATP/dUTP/dCTP/dGTP, ( 6 ) 2′- O -methyl-dATP, ( 7 ) 2′- O -methyl-dUTP, ( 8 ) 2′- O -methyl-dCTP, ( 9 ) 2′- O -methyl-dGTP, ( 10 ) mix of 2′- O -methyl-dATP/dUTP/dCTP/dGTP, ( 11 ) 2′-azido-2′-dATP, ( 12 ) 2′-azido-2′-dUTP, ( 13 ) 2′-azido-2′-dCTP, ( 14 ) 2′-azido-2′-dGTP, ( 15 ) mix of 2′-azido-2′-dATP/dUTP/dCTP/dGTP, ( 16 ) 2′-fluoro-dATP, ( 17 ) 2′-fluoro-dUTP, ( 18 ) 2′-fluoro-dCTP, ( 19 ) 2′-fluoro-dGTP, ( 20 ) 2′-fluoro-dTTP, ( 21 ) mix of 2′-fluoro-dATP/dUTP/dCTP/dGTP, ( 22 ) Ara-ATP (vidarabine triphosphate), ( 23 ) Ara-CTP (cytarabine triphosphate), ( 24 ) mix of Ara-ATP and Ara-CTP, ( 25 ) ϵ-ATP, ( 26 ) 2-aminopurine riboside triphosphate. The reactions were performed in the same conditions as the natural NTPs in presence of Mn 2+ .

Article Snippet: The following nucleotides were purchased from Jena Bioscience: 5-ethynyl-UTP, 2-aminopurine-ribonucleotide-5′-triphosphate, 2′- O -methyl-CTP, 2′-O-methyl-ATP, ara- CTP, ara- ATP, ϵ-ATP, 2′-fluoro-ATP, 2′-fluoro-CTP, 2′-fluoro-UTP and 2′-fluoro-GTP.

Techniques: Modification