ntd Search Results


rnapolii ser5p cst  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rnapolii ser5p cst
A: IGV snapshot depicting ChIP profiles of Mll-N, H3K4me3, RNAPolII (total), <t>RNAPolII-Ser5P,</t> and RNAPolII-Ser2P at the promoter of the Set target Med23 . B: Metagene plots across all Set activated genes reveal a specific loss of Mll and elongating RNA polymerase from chromatin after Set depletion. C: Only promoter recruited PP2A can repress the MLL responsive human Meis1 promoter. The reporter construct was co-transfected with expression constructs as indicated and luciferase results were determined in triplicate experiments. D: Recruitment of Set and PP2A to the endogenous Meis1 promoter in myeloid precursor cells. Effectors were targeted to the murine Meis1 promoter in MLL-ENL transformed cells by a fusion with catalytically inactive Cas9 (dCas9) in combination either with a non-targeting (vec) or two Meis1 promoter specific sgRNAs. Meis1 output was measured by RT-qPCR in triplicates.
Rnapolii Ser5p Cst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntd/Rpb1+NTD+Rabbit+mAb/bio_rxiv__64898__2026__02__26__708410-157-27-28
Average 96 stars, based on 1 article reviews
rnapolii ser5p cst - by Bioz Stars, 2026-08
96/100 stars
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anti sars cov 2 s protein  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc anti sars cov 2 s protein
A: IGV snapshot depicting ChIP profiles of Mll-N, H3K4me3, RNAPolII (total), <t>RNAPolII-Ser5P,</t> and RNAPolII-Ser2P at the promoter of the Set target Med23 . B: Metagene plots across all Set activated genes reveal a specific loss of Mll and elongating RNA polymerase from chromatin after Set depletion. C: Only promoter recruited PP2A can repress the MLL responsive human Meis1 promoter. The reporter construct was co-transfected with expression constructs as indicated and luciferase results were determined in triplicate experiments. D: Recruitment of Set and PP2A to the endogenous Meis1 promoter in myeloid precursor cells. Effectors were targeted to the murine Meis1 promoter in MLL-ENL transformed cells by a fusion with catalytically inactive Cas9 (dCas9) in combination either with a non-targeting (vec) or two Meis1 promoter specific sgRNAs. Meis1 output was measured by RT-qPCR in triplicates.
Anti Sars Cov 2 S Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntd/SARS-CoV-2+Spike+Protein+(S1-NTD)+Antibody/pm40565006-203-42-47
Average 94 stars, based on 1 article reviews
anti sars cov 2 s protein - by Bioz Stars, 2026-08
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monomeric sars cov 2 s1 protein  (ACROBiosystems)
95
ACROBiosystems monomeric sars cov 2 s1 protein
A: IGV snapshot depicting ChIP profiles of Mll-N, H3K4me3, RNAPolII (total), <t>RNAPolII-Ser5P,</t> and RNAPolII-Ser2P at the promoter of the Set target Med23 . B: Metagene plots across all Set activated genes reveal a specific loss of Mll and elongating RNA polymerase from chromatin after Set depletion. C: Only promoter recruited PP2A can repress the MLL responsive human Meis1 promoter. The reporter construct was co-transfected with expression constructs as indicated and luciferase results were determined in triplicate experiments. D: Recruitment of Set and PP2A to the endogenous Meis1 promoter in myeloid precursor cells. Effectors were targeted to the murine Meis1 promoter in MLL-ENL transformed cells by a fusion with catalytically inactive Cas9 (dCas9) in combination either with a non-targeting (vec) or two Meis1 promoter specific sgRNAs. Meis1 output was measured by RT-qPCR in triplicates.
Monomeric Sars Cov 2 S1 Protein, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntd/Biotinylated+SARS-CoV-2+S1+protein+NTD%2C+His%2CAvitag/pm38961245-291-25-32
Average 95 stars, based on 1 article reviews
monomeric sars cov 2 s1 protein - by Bioz Stars, 2026-08
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pro caspase 8  (Cell Signaling Technology Inc)
86
Cell Signaling Technology Inc pro caspase 8
A: IGV snapshot depicting ChIP profiles of Mll-N, H3K4me3, RNAPolII (total), <t>RNAPolII-Ser5P,</t> and RNAPolII-Ser2P at the promoter of the Set target Med23 . B: Metagene plots across all Set activated genes reveal a specific loss of Mll and elongating RNA polymerase from chromatin after Set depletion. C: Only promoter recruited PP2A can repress the MLL responsive human Meis1 promoter. The reporter construct was co-transfected with expression constructs as indicated and luciferase results were determined in triplicate experiments. D: Recruitment of Set and PP2A to the endogenous Meis1 promoter in myeloid precursor cells. Effectors were targeted to the murine Meis1 promoter in MLL-ENL transformed cells by a fusion with catalytically inactive Cas9 (dCas9) in combination either with a non-targeting (vec) or two Meis1 promoter specific sgRNAs. Meis1 output was measured by RT-qPCR in triplicates.
Pro Caspase 8, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntd/SARS-CoV-2+Spike+Protein+Multi-Domain+(S1-NTD%2C+RBD%2C+S1%2C+S2)+Serological+IgG+ELISA+Kit/pmc07245827-70-43-63
Average 86 stars, based on 1 article reviews
pro caspase 8 - by Bioz Stars, 2026-08
86/100 stars
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42172s  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc 42172s
Resources.
42172s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntd/SARS-CoV-2+Spike+Protein+(S1-NTD)+Mouse+mAb/pmc10798645-12-10-6
Average 92 stars, based on 1 article reviews
42172s - by Bioz Stars, 2026-08
92/100 stars
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bc047283 1 npc1l1 encoding plasmid addgene plasmid  (Addgene inc)
86
Addgene inc bc047283 1 npc1l1 encoding plasmid addgene plasmid
Resources.
Bc047283 1 Npc1l1 Encoding Plasmid Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntd/pfast+NPC1L1(NTD)+(Plasmid+%2337366)/pm29149604-303-103-107
Average 86 stars, based on 1 article reviews
bc047283 1 npc1l1 encoding plasmid addgene plasmid - by Bioz Stars, 2026-08
86/100 stars
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cul1 plasmid  (Addgene inc)
88
Addgene inc cul1 plasmid
FBXO45 facilitates assembly of a ubiquitin ligase complex containing SKP1 and PAM. A and B, schematics for different constructs tested for PAM (A) and FBXO45 (B). Annotated and highlighted in color are domains in PAM and FBXO45. C, coIP from transfected 293 cells showing HA–SKP1 binds GFP–FBXO45 but fails to bind GFP–PAM N-terminal (-term), GFP–PAM central, and GFP–PAM C-terminal constructs. D, coIP of HA–SKP1 with GFP–PAM D5 only occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM D5 (lower coIP panel). E, HA–SKP1 does not coIP with full-length GFP–PAM, but binding occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM (lower coIP panel). F, coIP showing HA–SKP1 binds the F-box domain of FBXO45. GFP–PAM (G) and FLAG–FBXO45 (H) fail to coIP with <t>MYC–CUL1.</t> I, summary showing interactions between SKP1, FBXO45, and PAM. C–H, shown are representatives of at least three independent experiments IP, immunoprecipitation.
Cul1 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntd/pAL-hCul1-NTD+(Plasmid+%2329518)/pmc06130950-725-20-22
Average 88 stars, based on 1 article reviews
cul1 plasmid - by Bioz Stars, 2026-08
88/100 stars
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mbp orf24 ntd wt  (Addgene inc)
91
Addgene inc mbp orf24 ntd wt
FBXO45 facilitates assembly of a ubiquitin ligase complex containing SKP1 and PAM. A and B, schematics for different constructs tested for PAM (A) and FBXO45 (B). Annotated and highlighted in color are domains in PAM and FBXO45. C, coIP from transfected 293 cells showing HA–SKP1 binds GFP–FBXO45 but fails to bind GFP–PAM N-terminal (-term), GFP–PAM central, and GFP–PAM C-terminal constructs. D, coIP of HA–SKP1 with GFP–PAM D5 only occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM D5 (lower coIP panel). E, HA–SKP1 does not coIP with full-length GFP–PAM, but binding occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM (lower coIP panel). F, coIP showing HA–SKP1 binds the F-box domain of FBXO45. GFP–PAM (G) and FLAG–FBXO45 (H) fail to coIP with <t>MYC–CUL1.</t> I, summary showing interactions between SKP1, FBXO45, and PAM. C–H, shown are representatives of at least three independent experiments IP, immunoprecipitation.
Mbp Orf24 Ntd Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntd/pMAL-c2X-ORF24-NTD+(Plasmid+%23138465)/pmc07498053-156-5-7
Average 91 stars, based on 1 article reviews
mbp orf24 ntd wt - by Bioz Stars, 2026-08
91/100 stars
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orf24 ntd  (Addgene inc)
91
Addgene inc orf24 ntd
FBXO45 facilitates assembly of a ubiquitin ligase complex containing SKP1 and PAM. A and B, schematics for different constructs tested for PAM (A) and FBXO45 (B). Annotated and highlighted in color are domains in PAM and FBXO45. C, coIP from transfected 293 cells showing HA–SKP1 binds GFP–FBXO45 but fails to bind GFP–PAM N-terminal (-term), GFP–PAM central, and GFP–PAM C-terminal constructs. D, coIP of HA–SKP1 with GFP–PAM D5 only occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM D5 (lower coIP panel). E, HA–SKP1 does not coIP with full-length GFP–PAM, but binding occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM (lower coIP panel). F, coIP showing HA–SKP1 binds the F-box domain of FBXO45. GFP–PAM (G) and FLAG–FBXO45 (H) fail to coIP with <t>MYC–CUL1.</t> I, summary showing interactions between SKP1, FBXO45, and PAM. C–H, shown are representatives of at least three independent experiments IP, immunoprecipitation.
Orf24 Ntd, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntd/pGEX4T1-ORF24-NTD+(Plasmid+%23138464)/pmc07498053-155-9-7
Average 91 stars, based on 1 article reviews
orf24 ntd - by Bioz Stars, 2026-08
91/100 stars
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p6h sumo3 orf24 ntd strep  (Addgene inc)
91
Addgene inc p6h sumo3 orf24 ntd strep
FBXO45 facilitates assembly of a ubiquitin ligase complex containing SKP1 and PAM. A and B, schematics for different constructs tested for PAM (A) and FBXO45 (B). Annotated and highlighted in color are domains in PAM and FBXO45. C, coIP from transfected 293 cells showing HA–SKP1 binds GFP–FBXO45 but fails to bind GFP–PAM N-terminal (-term), GFP–PAM central, and GFP–PAM C-terminal constructs. D, coIP of HA–SKP1 with GFP–PAM D5 only occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM D5 (lower coIP panel). E, HA–SKP1 does not coIP with full-length GFP–PAM, but binding occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM (lower coIP panel). F, coIP showing HA–SKP1 binds the F-box domain of FBXO45. GFP–PAM (G) and FLAG–FBXO45 (H) fail to coIP with <t>MYC–CUL1.</t> I, summary showing interactions between SKP1, FBXO45, and PAM. C–H, shown are representatives of at least three independent experiments IP, immunoprecipitation.
P6h Sumo3 Orf24 Ntd Strep, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntd/p6H-SUMO3-ORF24-NTD-Strep+(Plasmid+%23138467)/pmc07498053-158-13-14
Average 91 stars, based on 1 article reviews
p6h sumo3 orf24 ntd strep - by Bioz Stars, 2026-08
91/100 stars
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3l a  (Addgene inc)
91
Addgene inc 3l a
FBXO45 facilitates assembly of a ubiquitin ligase complex containing SKP1 and PAM. A and B, schematics for different constructs tested for PAM (A) and FBXO45 (B). Annotated and highlighted in color are domains in PAM and FBXO45. C, coIP from transfected 293 cells showing HA–SKP1 binds GFP–FBXO45 but fails to bind GFP–PAM N-terminal (-term), GFP–PAM central, and GFP–PAM C-terminal constructs. D, coIP of HA–SKP1 with GFP–PAM D5 only occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM D5 (lower coIP panel). E, HA–SKP1 does not coIP with full-length GFP–PAM, but binding occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM (lower coIP panel). F, coIP showing HA–SKP1 binds the F-box domain of FBXO45. GFP–PAM (G) and FLAG–FBXO45 (H) fail to coIP with <t>MYC–CUL1.</t> I, summary showing interactions between SKP1, FBXO45, and PAM. C–H, shown are representatives of at least three independent experiments IP, immunoprecipitation.
3l A, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntd/pMAL-c2X-ORF24-NTD-3L_A+(Plasmid+%23138466)/pmc07498053-156-10-11
Average 91 stars, based on 1 article reviews
3l a - by Bioz Stars, 2026-08
91/100 stars
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sars cov 2 spike protein s1 ntd  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc sars cov 2 spike protein s1 ntd
FBXO45 facilitates assembly of a ubiquitin ligase complex containing SKP1 and PAM. A and B, schematics for different constructs tested for PAM (A) and FBXO45 (B). Annotated and highlighted in color are domains in PAM and FBXO45. C, coIP from transfected 293 cells showing HA–SKP1 binds GFP–FBXO45 but fails to bind GFP–PAM N-terminal (-term), GFP–PAM central, and GFP–PAM C-terminal constructs. D, coIP of HA–SKP1 with GFP–PAM D5 only occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM D5 (lower coIP panel). E, HA–SKP1 does not coIP with full-length GFP–PAM, but binding occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM (lower coIP panel). F, coIP showing HA–SKP1 binds the F-box domain of FBXO45. GFP–PAM (G) and FLAG–FBXO45 (H) fail to coIP with <t>MYC–CUL1.</t> I, summary showing interactions between SKP1, FBXO45, and PAM. C–H, shown are representatives of at least three independent experiments IP, immunoprecipitation.
Sars Cov 2 Spike Protein S1 Ntd, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ntd/SARS-CoV-2+Spike+S1-NTD+(16-316)+Recombinant+Protein/pmc09352574-350-20-25
Average 92 stars, based on 1 article reviews
sars cov 2 spike protein s1 ntd - by Bioz Stars, 2026-08
92/100 stars
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Image Search Results


A: IGV snapshot depicting ChIP profiles of Mll-N, H3K4me3, RNAPolII (total), RNAPolII-Ser5P, and RNAPolII-Ser2P at the promoter of the Set target Med23 . B: Metagene plots across all Set activated genes reveal a specific loss of Mll and elongating RNA polymerase from chromatin after Set depletion. C: Only promoter recruited PP2A can repress the MLL responsive human Meis1 promoter. The reporter construct was co-transfected with expression constructs as indicated and luciferase results were determined in triplicate experiments. D: Recruitment of Set and PP2A to the endogenous Meis1 promoter in myeloid precursor cells. Effectors were targeted to the murine Meis1 promoter in MLL-ENL transformed cells by a fusion with catalytically inactive Cas9 (dCas9) in combination either with a non-targeting (vec) or two Meis1 promoter specific sgRNAs. Meis1 output was measured by RT-qPCR in triplicates.

Journal: bioRxiv

Article Title: The nuclear oncoprotein SET is necessary for MLL/KMT2A binding and transcriptional elongation

doi: 10.64898/2026.02.26.708410

Figure Lengend Snippet: A: IGV snapshot depicting ChIP profiles of Mll-N, H3K4me3, RNAPolII (total), RNAPolII-Ser5P, and RNAPolII-Ser2P at the promoter of the Set target Med23 . B: Metagene plots across all Set activated genes reveal a specific loss of Mll and elongating RNA polymerase from chromatin after Set depletion. C: Only promoter recruited PP2A can repress the MLL responsive human Meis1 promoter. The reporter construct was co-transfected with expression constructs as indicated and luciferase results were determined in triplicate experiments. D: Recruitment of Set and PP2A to the endogenous Meis1 promoter in myeloid precursor cells. Effectors were targeted to the murine Meis1 promoter in MLL-ENL transformed cells by a fusion with catalytically inactive Cas9 (dCas9) in combination either with a non-targeting (vec) or two Meis1 promoter specific sgRNAs. Meis1 output was measured by RT-qPCR in triplicates.

Article Snippet: Set : Invitrogen (#MA5-35772); MLL: CST (#8178) mixed 1:1 with anti-MLLN, Upstate (Temecula, CA, #05-764) 10 μl AB-mix per 5×10e6 cells, H3K4me3: CST (#9751), RNAPolymeraseII: CST (#14958), RNAPolII-Ser5P: CST (#13499), RNAPolII-Ser2P: CST (#13523), Msk1-P (Ser376P): CST (#9591), H3S10P: CST (#53348), H3K9ac: CST (#9649), H3K14ac: CST (#7627), H3K27ac: CST (#8173), Cbp: CST (#7389), Creb-P (Ser133P): CST (#9198), MOZ: Invitrogen (PA5-68046) mixed 1:1 with Invitrogen (PA5-103467), HA: CST (#3724), flag: Merck (F1804).

Techniques: Construct, Transfection, Expressing, Luciferase, Transformation Assay, Quantitative RT-PCR

Resources.

Journal: PLOS Pathogens

Article Title: Mutations accumulated in the Spike of SARS-CoV-2 Omicron allow for more efficient counteraction of the restriction factor BST2/Tetherin

doi: 10.1371/journal.ppat.1011912

Figure Lengend Snippet: Resources.

Article Snippet: Mouse mAb anti-SARS-CoV-2 S (S1-NTD) , Cell Signaling , Cat# 42172S.

Techniques: Virus, Variant Assay, Recombinant, Plasmid Preparation, In Vitro, Transfection, cDNA Synthesis, Lysis, Magnetic Beads, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, Software

FBXO45 facilitates assembly of a ubiquitin ligase complex containing SKP1 and PAM. A and B, schematics for different constructs tested for PAM (A) and FBXO45 (B). Annotated and highlighted in color are domains in PAM and FBXO45. C, coIP from transfected 293 cells showing HA–SKP1 binds GFP–FBXO45 but fails to bind GFP–PAM N-terminal (-term), GFP–PAM central, and GFP–PAM C-terminal constructs. D, coIP of HA–SKP1 with GFP–PAM D5 only occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM D5 (lower coIP panel). E, HA–SKP1 does not coIP with full-length GFP–PAM, but binding occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM (lower coIP panel). F, coIP showing HA–SKP1 binds the F-box domain of FBXO45. GFP–PAM (G) and FLAG–FBXO45 (H) fail to coIP with MYC–CUL1. I, summary showing interactions between SKP1, FBXO45, and PAM. C–H, shown are representatives of at least three independent experiments IP, immunoprecipitation.

Journal: The Journal of Biological Chemistry

Article Title: PAM forms an atypical SCF ubiquitin ligase complex that ubiquitinates and degrades NMNAT2

doi: 10.1074/jbc.RA118.002176

Figure Lengend Snippet: FBXO45 facilitates assembly of a ubiquitin ligase complex containing SKP1 and PAM. A and B, schematics for different constructs tested for PAM (A) and FBXO45 (B). Annotated and highlighted in color are domains in PAM and FBXO45. C, coIP from transfected 293 cells showing HA–SKP1 binds GFP–FBXO45 but fails to bind GFP–PAM N-terminal (-term), GFP–PAM central, and GFP–PAM C-terminal constructs. D, coIP of HA–SKP1 with GFP–PAM D5 only occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM D5 (lower coIP panel). E, HA–SKP1 does not coIP with full-length GFP–PAM, but binding occurs in the presence of FLAG–FBXO45 (upper coIP panel). HA–SKP1 coIPs with FLAG–FBXO45 in the presence or absence of GFP–PAM (lower coIP panel). F, coIP showing HA–SKP1 binds the F-box domain of FBXO45. GFP–PAM (G) and FLAG–FBXO45 (H) fail to coIP with MYC–CUL1. I, summary showing interactions between SKP1, FBXO45, and PAM. C–H, shown are representatives of at least three independent experiments IP, immunoprecipitation.

Article Snippet: We thank Dr. Manfred Gessler for HA–SKP1 plasmid, Dr. Aaron DiAntonio for NMNAT2–MYC-HIS 6 plasmid, and Dr. Ning Zheng for CUL1 plasmid (Addgene 29518).

Techniques: Ubiquitin Proteomics, Construct, Transfection, Binding Assay, Immunoprecipitation

Comparison of noncanonical PAM/FBXO45/SKP1 complex with traditional SCF ubiquitin ligase complex. A, summary of biochemistry underpinning formation of the PAM/FBXO45/SKP1 complex, which ubiquitinates NMNAT2 and targets it for proteasomal degradation. The F-box protein FBXO45 binds directly to the FBD1 domain of PAM and recognizes NMNAT2 as a target for ubiquitination. SKP1 acts as an auxiliary component that increases FBXO45 binding to NMNAT2. B, diagram of traditional SCF complex formed by RBX1/CUL1/SKP1/SKP2 (adapted from Zheng et al. (8)).

Journal: The Journal of Biological Chemistry

Article Title: PAM forms an atypical SCF ubiquitin ligase complex that ubiquitinates and degrades NMNAT2

doi: 10.1074/jbc.RA118.002176

Figure Lengend Snippet: Comparison of noncanonical PAM/FBXO45/SKP1 complex with traditional SCF ubiquitin ligase complex. A, summary of biochemistry underpinning formation of the PAM/FBXO45/SKP1 complex, which ubiquitinates NMNAT2 and targets it for proteasomal degradation. The F-box protein FBXO45 binds directly to the FBD1 domain of PAM and recognizes NMNAT2 as a target for ubiquitination. SKP1 acts as an auxiliary component that increases FBXO45 binding to NMNAT2. B, diagram of traditional SCF complex formed by RBX1/CUL1/SKP1/SKP2 (adapted from Zheng et al. (8)).

Article Snippet: We thank Dr. Manfred Gessler for HA–SKP1 plasmid, Dr. Aaron DiAntonio for NMNAT2–MYC-HIS 6 plasmid, and Dr. Ning Zheng for CUL1 plasmid (Addgene 29518).

Techniques: Comparison, Ubiquitin Proteomics, Binding Assay