Journal: Frontiers in Cellular Neuroscience

Article Title: BK Channel-Mediated Microglial Phagocytosis Alleviates Neurological Deficit After Ischemic Stroke

doi: 10.3389/fncel.2021.683769

Figure Lengend Snippet: BK channel activator NS19504 promoted microglial phagocytosis after OGD in primary culture . (A) Representative images of beads (red) phagocytosed by CD11b + microglia (green) after 1 h of OGD. Control group: primary microglia cultured with basic microglia medium; NS19504 group: primary microglia cultured with 10 μM NS19504 in basic microglia medium; Paxilline group: primary microglia cultured with 1 μM Paxilline in basic microglia medium. Scale bar = 25 μm. (B) Bar graph showed that semiquantitative data of the relative bead density versus microglia. Data are mean + SE, n = 3 per group. * p

Article Snippet: The injection concentrations of Paxilline and NS19504 were 500 μM and 5 mM, respectively, having the same concentration of DMSO [10% ( )].

Techniques: Cell Culture

Journal: Frontiers in Cellular Neuroscience

Article Title: BK Channel-Mediated Microglial Phagocytosis Alleviates Neurological Deficit After Ischemic Stroke

doi: 10.3389/fncel.2021.683769

Figure Lengend Snippet: Activation of BK channels ameliorated stroke outcomes. (A) Experiment design of the animal experiment. Three days before tMCAO, mice were subjected behavior training. After a 90-min tMCAO surgery, mice were divided randomly into four groups (sham, DMSO, NS19504, and Paxilline). At 1, 3, 7, and 14 days, behavior tests were performed, and protein and RNA samples were collected. (B) Weight changes of mice in three groups at 1, 2, 3, 7, and 14 days after tMCAO. (C) Modified Neurological Severity Score of three groups at 1, 3, 7, and 14 days after tMCAO. (D) Average score per second of three groups of hinging wire at 3 days after tMCAO. (E) Survivorship curve during 14 days of three groups after tMCAO. Data are mean + SE, n = 21–23 per group at 1 day, n = 17–23 per group at 3 days, n = 8–13 per group at 7 days, n = 3–9 per group at 14 days. *NS19504 vs. Paxilline group, ** p

Article Snippet: The injection concentrations of Paxilline and NS19504 were 500 μM and 5 mM, respectively, having the same concentration of DMSO [10% ( )].

Techniques: Activation Assay, Mouse Assay, Modification

Journal: Frontiers in Cellular Neuroscience

Article Title: BK Channel-Mediated Microglial Phagocytosis Alleviates Neurological Deficit After Ischemic Stroke

doi: 10.3389/fncel.2021.683769

Figure Lengend Snippet: Activation of BK channels promoted the phagocytic function of microglia after tMCAO and decreased neuronal apoptosis . (A) Representative photomicrograms of IBA1 + microglia (green) and TUNEL + apoptotic cells (red) in the peri-infarct region of brain slice in the DMSO, NS19504, and Paxilline groups at 3 days after tMCAO. Scale bar = 50 μm. (B) Bar graph shows quantitative analysis of the number of IBA1 + TUNEL + cells per field. Data are mean + SE, n = 3 per group. *vs. DMSO group, ** p

Article Snippet: The injection concentrations of Paxilline and NS19504 were 500 μM and 5 mM, respectively, having the same concentration of DMSO [10% ( )].

Techniques: Activation Assay, TUNEL Assay, Slice Preparation

Journal: Frontiers in Cellular Neuroscience

Article Title: BK Channel-Mediated Microglial Phagocytosis Alleviates Neurological Deficit After Ischemic Stroke

doi: 10.3389/fncel.2021.683769

Figure Lengend Snippet: Inflammatory factors and phosphorylated-ERK expressions under different treatments at 3 days after tMCAO . (A) Bar graph represents the relative mRNA levels of inflammatory factors including TNF-α, TGF-β, IL-10, IL-6, IL-1β, and IL-1α. Data are mean + SE, n = 3 per group. (B) Representative image of Western blot of phosphorylated-ERK1/2 (p-ERK), total-ERK1/2 (t-ERK), and β-actin at 3 days after tMCAO. (C) Bar graph showing the relative protein levels of p -ERK vs. t-ERK at 3 day after tMCAO. Data are mean + SE, n = 9 per group. *NS19504 vs. Paxilline group, * p

Article Snippet: The injection concentrations of Paxilline and NS19504 were 500 μM and 5 mM, respectively, having the same concentration of DMSO [10% ( )].

Techniques: Western Blot