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Image Search Results
Journal: Genes
Article Title: Suppression of Notch Signaling Stimulates Progesterone Synthesis by Enhancing the Expression of NR5A2 and NR2F2 in Porcine Granulosa Cells
doi: 10.3390/genes11020120
Figure Lengend Snippet: Effects of NR2F2 knockdown on progesterone biosynthesis. ( A ) gene expression of NR2F2 after siRNA transfection; ( B ) Effects of NR2F2 knockdown on progesterone secretion; ( C ) gene expression of key factors (StAR, Cyp11a1, HSD3B, and NPC1) involved in progesterone biosynthesis; ( D , E ) protein expression of NR5A2, StAR, Cyp11a1, and HSD3B. Primary pGCs were transfected with NR2F2 siRNA (siNR2F2) or scramble siRNA (siNC). Six hours later, the medium was changed to fresh medium with DMSO or DAPT, followed by further culture for 48 h. * means significant difference at p < 0.05.
Article Snippet: The proteins were subsequently transferred to a polyvinylidene fluoride membrane (Millipore, Darmstadt, Germany) and probed with primary antibodies against StAR (DF6192, Affinity Biosciences, Changzhou, China), Cyp11a1 (DF6569, Affinity Biosciences), HSD3B (DF6639, Affinity Biosciences), ERK1/2 (4695T, Cell Signaling Technology, Danvers, MA, USA), pERK1/2 (4370T, Cell Signaling),
Techniques: Knockdown, Gene Expression, Transfection, Expressing
Journal: Genes
Article Title: Suppression of Notch Signaling Stimulates Progesterone Synthesis by Enhancing the Expression of NR5A2 and NR2F2 in Porcine Granulosa Cells
doi: 10.3390/genes11020120
Figure Lengend Snippet: Effects of NR5A2 and NR2F2 knockdown on the expression of Notch receptors and ligands. ( A ) Effects of NR5A2 and NR2F2 on the expression of Notch receptors (Notch2 and Notch3) and Notch ligands (Dll1 and Jagged1). Primary pGCs were transfected with NR5A2 siRNA (siNR5A2), NR2F2 siRNA (siNR2F2), or scramble siRNA (siNC). ( B ) Schematic representation of the Notch/NR5A2/NR2F2 pathway in regulating steroidogenesis pGCs. * means significant difference at p < 0.05.
Article Snippet: The proteins were subsequently transferred to a polyvinylidene fluoride membrane (Millipore, Darmstadt, Germany) and probed with primary antibodies against StAR (DF6192, Affinity Biosciences, Changzhou, China), Cyp11a1 (DF6569, Affinity Biosciences), HSD3B (DF6639, Affinity Biosciences), ERK1/2 (4695T, Cell Signaling Technology, Danvers, MA, USA), pERK1/2 (4370T, Cell Signaling),
Techniques: Knockdown, Expressing, Transfection
Journal: Cell & Bioscience
Article Title: Inhibition of exosome biogenesis affects cell motility in heterogeneous sub-populations of paediatric-type diffuse high-grade gliomas
doi: 10.1186/s13578-023-01166-5
Figure Lengend Snippet: Exosome miRNAs in OPBG-DIPG002 and OPBG-GBM002 single-cell-derived clones
Article Snippet: Quantitative real-time PCR (q-PCR) was performed using TaqMan Fast Universal PCR Master Mix (
Techniques: