Journal: NPJ biofilms and microbiomes

Article Title: Alleviation of Limosilactobacillus reuteri in polycystic ovary syndrome protects against circadian dysrhythmia-induced dyslipidemia via capric acid and GALR1 signaling.

doi: 10.1038/s41522-023-00415-2

Figure Lengend Snippet: Fig. 2 Liver transcriptome analysis of L. reuteri-treated darkness rats. Heatmaps displaying 36 highly expressed genes (a) and 76 lowly expressed genes (b) in the liver of darkness rats compared with control and DL.reuteri rats through DEG analysis using Ballgown software (| fold change | > 0.6 in the log2 ratio value, raw P < 0.05). c Top terms from GO analysis (above) and terms from KEGG analysis (below) of the 112 differential genes in the DEG analysis. The P values of GO and KEGG analyses were determined on the DAVID website. d Visualization of the top GO and KEGG terms related with lipid metabolism and circadian rhythm in the DEG analysis. e Spearman rank correlations between module eigengenes (ME) and clinical biochemical index in the WGCNA analysis (|ρ | > 0.3, *P < 0.05). f Visualization of the top GO and KEGG terms related with lipid metabolism and circadian rhythm of purple module genes (102) in the WGCNA analysis. Circle, genes; Square, KEGG terms; Hexagon, GO terms. The bigger the square or hexagon, the more genes involved. g Crosstalk among different groups of genes identified the possible target genes taking essential roles in the lipid metabolism of L. reuteri-treated darkness rats. h mRNA abundances of Galr1, Galr2, Nr1d1, Nr1d2, Insig2, Srebf1, Lxra, and Rxra in rat liver detected by qPCR. β-Actin was used as a loading control for qPCR analyses. i Serum galanin concentration detected by ELISA. Statistical analysis (h, i) was performed with one-way ANOVA followed by Newman–Keuls multiple comparison test. n = 8 per group. Data present means ± SEM. *P < 0.05, **P < 0.01.

Article Snippet: Published in partnership with Nanyang Technological University npj Biofilms and Microbiomes (2023) 47 GALR2 antibody (1:500; #26459-1-AP, Proteintech, Wuhan, China), NR1D1 antibody (1:1000, #13418 S, Cell Signaling Technology, Massachusetts, USA; 1:1000, #14506-1-AP, Proteintech), NR1D2 antibody (1:500; #13906-1-AP, Proteintech), SREBP1 antibody (1:1000; #41878, Signalway Antibody), LXRa antibody (1:1000; #ab176323, Abcam, Cambridge, UK), RXRa antibody (1:1000; #ab125001, Abcam), INSIG2 antibody (1:500; #24766-1, Proteintech), P-AKT antibody (1:1000; #4060, Cell Signaling Technology), Fig. 8 Proposed mechanisms for the amelioration of L. reuteri on dyslipidemia in circadian dysrhythmia-induced PCOS-like rats.

Techniques: Control, Software, Concentration Assay, Enzyme-linked Immunosorbent Assay, Comparison

Journal: NPJ biofilms and microbiomes

Article Title: Alleviation of Limosilactobacillus reuteri in polycystic ovary syndrome protects against circadian dysrhythmia-induced dyslipidemia via capric acid and GALR1 signaling.

doi: 10.1038/s41522-023-00415-2

Figure Lengend Snippet: Fig. 8 Proposed mechanisms for the amelioration of L. reuteri on dyslipidemia in circadian dysrhythmia-induced PCOS-like rats. Left, circadian dysrhythmia due to constant darkness resulted in dyslipidemia and reproductive hallmarks of PCOS in rats. Elevated galanin-GALR1 induced by darkness exposure functioned as an upstream factor of PI3K/AKT pathway and further suppressed NR1D1-induced SREBF1 transcription and translation, thus inducing hepatic lipid accumulation in PCOS-like rats. Right, L. reuteri supplementation ameliorated dyslipidemia and reproductive hallmarks in circadian dysrhythmia-induced PCOS-like rats. L. reuteri restructured microbiome-metabolome network in darkness rats ameliorating the abundance of Lactobacillus, Clostridium sensu stricto 1, Ruminococcaceae UCG-010, and Family XIII AD3011 group, followed by varied serum levels of cortisol, cis-9-palmitoleic acid, 13-methylmyristic acid, capric acid, and dUMP. Notably, capric acid mediated the inhibition of L. reuteri on hepatic GALR1-PI3K/AKT-NR1D1-SREBP1 pathway, which eventually alleviated dyslipidemia.

Article Snippet: Published in partnership with Nanyang Technological University npj Biofilms and Microbiomes (2023) 47 GALR2 antibody (1:500; #26459-1-AP, Proteintech, Wuhan, China), NR1D1 antibody (1:1000, #13418 S, Cell Signaling Technology, Massachusetts, USA; 1:1000, #14506-1-AP, Proteintech), NR1D2 antibody (1:500; #13906-1-AP, Proteintech), SREBP1 antibody (1:1000; #41878, Signalway Antibody), LXRa antibody (1:1000; #ab176323, Abcam, Cambridge, UK), RXRa antibody (1:1000; #ab125001, Abcam), INSIG2 antibody (1:500; #24766-1, Proteintech), P-AKT antibody (1:1000; #4060, Cell Signaling Technology), Fig. 8 Proposed mechanisms for the amelioration of L. reuteri on dyslipidemia in circadian dysrhythmia-induced PCOS-like rats.

Techniques: Inhibition

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Helicobacter pylori –Induced Rev-erbα Fosters Gastric Bacteria Colonization by Impairing Host Innate and Adaptive Defense

doi: 10.1016/j.jcmgh.2021.02.013

Figure Lengend Snippet: Antibodies and Other Reagents

Article Snippet: SimpleChip human NR1D1 promoter primers (13413s) , Cell Signaling Technology.

Techniques: Flow Cytometry, Immunohistochemical staining, Staining, Immunofluorescence, Blocking Assay, Control, Western Blot, Enzyme-linked Immunosorbent Assay, Protein-Protein interactions, Inhibition, Phospho-proteomics, Luciferase, Reporter Assay, Plasmid Preparation, Purification, Cell Isolation, Pore Size, Protein Extraction, Transfection, Real-time Polymerase Chain Reaction, Recombinant

Journal: Breast Cancer Research : BCR

Article Title: High expression of NR1D1 is associated with good prognosis in triple-negative breast cancer patients treated with chemotherapy

doi: 10.1186/s13058-019-1197-x

Figure Lengend Snippet: Characteristics of breast cancer patients and their NR1D1 expression level

Article Snippet: The tissue sections were then incubated for 1 h at room temperature with a primary antibody against NR1D1 (H00009572-M02, Novus Biologicals LCC, Littleton, CO, USA) diluted to a final concentration of 1:1000.

Techniques: Expressing, Biomarker Discovery, Amplification, Adjuvant

Journal: Breast Cancer Research : BCR

Article Title: High expression of NR1D1 is associated with good prognosis in triple-negative breast cancer patients treated with chemotherapy

doi: 10.1186/s13058-019-1197-x

Figure Lengend Snippet: Association of NR1D1 expression with clinical outcome in breast cancer patients. Kaplan−Meier curves for OS and DFS according to NR1D1 expression in all breast cancer patients and in breast cancer patients receiving chemotherapy. Three or two patients without OS or DFS information, respectively, were excluded from the analysis

Article Snippet: The tissue sections were then incubated for 1 h at room temperature with a primary antibody against NR1D1 (H00009572-M02, Novus Biologicals LCC, Littleton, CO, USA) diluted to a final concentration of 1:1000.

Techniques: Expressing

Journal: Breast Cancer Research : BCR

Article Title: High expression of NR1D1 is associated with good prognosis in triple-negative breast cancer patients treated with chemotherapy

doi: 10.1186/s13058-019-1197-x

Figure Lengend Snippet: Prognostic significance of NR1D1 expression in TNBC patients treated with chemotherapy. Kaplan−Meier curves for OS and DFS according to NR1D1 expression in molecular subtypes of breast cancer patients treated with chemotherapy. A patient with no subtype information was excluded from the analysis

Article Snippet: The tissue sections were then incubated for 1 h at room temperature with a primary antibody against NR1D1 (H00009572-M02, Novus Biologicals LCC, Littleton, CO, USA) diluted to a final concentration of 1:1000.

Techniques: Expressing

Journal: Breast Cancer Research : BCR

Article Title: High expression of NR1D1 is associated with good prognosis in triple-negative breast cancer patients treated with chemotherapy

doi: 10.1186/s13058-019-1197-x

Figure Lengend Snippet: Univariate analysis of OS and DFS in the TNBC patients treated with chemotherapy

Article Snippet: The tissue sections were then incubated for 1 h at room temperature with a primary antibody against NR1D1 (H00009572-M02, Novus Biologicals LCC, Littleton, CO, USA) diluted to a final concentration of 1:1000.

Techniques: Expressing, Biomarker Discovery

Journal: Breast Cancer Research : BCR

Article Title: High expression of NR1D1 is associated with good prognosis in triple-negative breast cancer patients treated with chemotherapy

doi: 10.1186/s13058-019-1197-x

Figure Lengend Snippet: Multivariate analysis of OS and DFS in the TNBC patients treated with chemotherapy

Article Snippet: The tissue sections were then incubated for 1 h at room temperature with a primary antibody against NR1D1 (H00009572-M02, Novus Biologicals LCC, Littleton, CO, USA) diluted to a final concentration of 1:1000.

Techniques: Expressing