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Image Search Results
Journal: Bioconjugate chemistry
Article Title: Probing the Broad-Spectrum Virus-Neutralizing Epitopes Using the High-Density Oligomannose-Conjugates and Carbohydrate Microarrays
doi: 10.1021/acs.bioconjchem.5c00361
Figure Lengend Snippet: Overexpression of GNA-glyco-epitopes by SARS-CoV-2 and other human coronaviruses. (A) Relative expression levels of GNA + -glyco-epitopes expressed by a panel of viral glycoproteins as compared to several human glycoproteins. (B) SARS-CoV-2 neutralization by GNA. We applied a pseudotyping lentiviral neutralization kit from the American Type Culture Collection/Biodefense and Emerging Infections Research Resources Repository (ATCC/BEI) (NR-52948) to determine the potency of GNA’s SARS-CoV-2-neutralizing activities in HEK-293T-hACE2 (human angiotensin-converting enzyme 2) cells. Inhibition curves were plotted with % of inhibition on the Y -axis and GNA concentration on the X -axis. The blue dots represent means of triplicate detections with error bars of one standard error. The 4PL model was used to fit the viral infectivity curves and determine the IC 50 values.
Article Snippet: A
Techniques: Over Expression, Expressing, Neutralization, Inhibition, Concentration Assay, Infection