Journal: Journal of Hepatocellular Carcinoma

Article Title: Coilin Affects the Prognosis of Hepatocellular Carcinoma Through Cell Cycle and Apoptosis

doi: 10.2147/JHC.S500119

Figure Lengend Snippet: Expression of COIL in HCC cell lines and HCC tissue from hospital. The expression of COIL in liver cancer cell lines huh7, hepG2 and hep3B was higher than that in L02 by immunohistochemistry ( A ) and Western blotting ( B ). ( C ) The relative expression of COIL in HCC was significantly higher than that in normal liver tissue by qRT-PCR (***p value < 0.001). ( D ) The expression of COIL in HCC tissue was higher than that in normal liver tissue. ( E, F ) Representative immunohistochemistry results of low or high COIL expression. ( G ) High COIL expression was associated with poor overall survival.

Article Snippet: The human normal liver cell line L02 was obtained from Beijing Baiao Bowei Biotechnology Co., Ltd.

Techniques: Expressing, Immunohistochemistry, Western Blot, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling

doi: 10.1038/srep35131

Figure Lengend Snippet: Changes in the geometrical parameters of HCCs and NHs before and after CII.

Article Snippet: Five HCC lines, HepG2, Smmc-7721, Huh-7, Mhcc-97L, and Mhcc-97H, and a control NH line, L02, were obtained from the China Center for Type Culture Collection (Shanghai, China).

Techniques: Cell Analysis, Single-cell Analysis

Journal: Scientific Reports

Article Title: Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling

doi: 10.1038/srep35131

Figure Lengend Snippet: ( A ) Cell shape vs mechanical properties. In detail, CPA vs E, in L02, r = 0.642, P = 0.00 < 0.01; in HepG2, r = 0.619, P = 0.00 < 0.01; in Smmc-7721, r = 0.519, P = 0.00 < 0.01; in Huh-7, r = 0.564, P = 0.00 < 0.01; in Mhcc-97L, r = 0.355, P = 0.005 < 0.01; ( B ) Cytoskeleton density vs mechanical properties. In detail, CD vs E, in L02, r = 0.872, P = 0.00 < 0.01; in HepG2, r = 0.790, P = 0.00 < 0.01; in Smmc-7721, r = 0.876, P = 0.00 < 0.01; in Huh-7, r = 0.866, P = 0.00 < 0.01; in Mhcc-97L, r = 0.724, P = 0.00 < 0.01; (C) Cell shape vs cytoskeleton density. In detail, CPA vs CD, in L02, r = 0.841, P = 0.00 < 0.01; in HepG2, r = 0.784, P = 0.00 < 0.01; in Smmc-7721, r = 0.700, P = 0.00 < 0.01; in Huh-7, r = 0.568, P = 0.00 < 0.01; in Mhcc-97L, r = 0.501, P = 0.003 < 0.01; ( D ) Caspase-3 protein levels vs mechanical properties. In detail, CPLs vs E, in L02, r = −0.811, P = 0.00 < 0.01; in HepG2, r = −0.784, P = 0.00 < 0.01; in Smmc-7721, r = −0.714, P = 0.00 < 0.01; in Huh-7, r = −0.747, P = 0.00 < 0.01; in Mhcc-97L, r = −0.701, P = 0.003 < 0.01.

Article Snippet: Five HCC lines, HepG2, Smmc-7721, Huh-7, Mhcc-97L, and Mhcc-97H, and a control NH line, L02, were obtained from the China Center for Type Culture Collection (Shanghai, China).

Techniques:

Journal: Cell Death & Disease

Article Title: Polyphyllin I suppresses the formation of vasculogenic mimicry via Twist1/VE-cadherin pathway

doi: 10.1038/s41419-018-0902-5

Figure Lengend Snippet: a Polyphyllin I exhibited the most obvious inhibitory effect on VM formation. b Structure of PPI. c Effects of PPI on the viability of SMMC7721, PLC, HepG2, Hep3B, and Bel7402 cells. PPI reduced the survival rate of HCC cells at relatively high concentrations and had slight influence on cell survival below 1 μM. d Cell cycle analysis of PPI in HCC cell lines. 0.1 μM PPI did not influence the cycle of HCC cells. e , f PPI inhibited the migration and invasion of HCC cells. g PPI decreased the number of pipe-like structures in SMMC7721 cells on Matrigel. h Time-lapse images of SMMC7721. PPI inhibited the pipe-like structure formation in SMMC7721 cells ( n = 3)

Article Snippet: HCC cell lines including SMMC7721, PLC, HepG2, Hep3B, and Bel7402 were purchased from Keygene BioTECH (Nanjing, China) and validated through a short tandem repeat-based method.

Techniques: Cell Cycle Assay, Migration

Journal: JAMA Ophthalmology

Article Title: Complement Proteins in the Retina in Cancer-Associated Retinopathy

doi: 10.1001/jamaophthalmol.2019.4405

Figure Lengend Snippet: Images show a normal human eye obtained at autopsy (A) and the eye of a deceased patient with CAR (B). The top panels show hematoxylin-eosin–stained (original magnification ×100) sections. The top image in B shows the severe loss of photoreceptor cells, which typified most of the retina in the patient with CAR. A few photoreceptor nuclei are seen to the right of the figure, as this is the boundary of a patch of remaining photoreceptors. The bottom image in A shows the anti-C3 antibody staining in a normal retina; the brown of the retinal pigment epithelium (RPE) is from its melanin. The bottom image in B, also stained with an anti-C3 antibody, shows a patch of remaining photoreceptors in the patient with CAR. Complement factor C3 is found in the photoreceptor inner and outer segments and the RPE. GCL indicates ganglion cell layer; INL, inner nuclear layer; and ONL, outer nuclear layer (photoreceptor nuclei).

Article Snippet: 4 We used sections of normal (without CAR) human eyes (obtained from Lions Eye Bank) as negative controls and normal human liver cells (obtained from Avaden) as positive controls.

Techniques: Staining

Journal: JAMA Ophthalmology

Article Title: Complement Proteins in the Retina in Cancer-Associated Retinopathy

doi: 10.1001/jamaophthalmol.2019.4405

Figure Lengend Snippet: A, A normal human eye obtained at autopsy. B, Eye of a deceased patient with CAR. Complement factor C9 (top row) is in the choriocapillaris of the normal eye, whereas C9 is also found in the photoreceptor inner and outer segments and the retinal pigment epithelium (RPE) of the eye with CAR. The staining of factor B (bottom row) was faint and thus inconclusive. GCL indicates ganglion cell layer; INL, inner nuclear layer; and ONL, outer nuclear layer (photoreceptor nuclei).

Article Snippet: 4 We used sections of normal (without CAR) human eyes (obtained from Lions Eye Bank) as negative controls and normal human liver cells (obtained from Avaden) as positive controls.

Techniques: Staining