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Image Search Results
Journal: Clinical Epigenetics
Article Title: Platelet mitochondrial DNA methylation: a potential new marker of cardiovascular disease
doi: 10.1186/s13148-015-0078-0
Figure Lengend Snippet: A representative gel following PCR amplification of mitochondrial and nuclear DNA sequences. Amplification of MT-CO1 , CDH1 , CDKN2A , and LINE-1 in extracted mtDNA (lanes 1 to 4) and control DNA from human adult normal peripheral blood leukocyte (Biochain, Newark, CA, USA) (lanes 6 to 9) were visualized on a 2% agarose gel. The fifth lane contains a DNA size marker.
Article Snippet: In addition to extracted
Techniques: Amplification, Control, Agarose Gel Electrophoresis, Marker
Journal: Clinical Epigenetics
Article Title: Platelet mitochondrial DNA methylation: a potential new marker of cardiovascular disease
doi: 10.1186/s13148-015-0078-0
Figure Lengend Snippet: Technical replicates and quantitative validation of mtDNA pyrosequencing assays. Spearman correlation coefficients ( r ) of the technical replicates (replicates 1 and 2) for the designed pyrosequencing assays were (a) 0.97 for MT-CO1 , (b) 0.93 for MT-CO2 , and (c) 0.76 for MT-CO3 . The plot curve is displayed with a 95% confidence band. The quantitative validation of 0% and 100% methylated DNA for the (d) MT-CO1 , (e) MT-CO2 , and (f) MT-CO3 assays (red circle) were compared with 0% and 100% control oligonucleotides (black rectangle).
Article Snippet: In addition to extracted
Techniques: Biomarker Discovery, Methylation, Control