Journal: Nucleic Acids Research

Article Title: The role of DNA damage response pathways in chromosome fragility in Fragile X syndrome

doi: 10.1093/nar/gkp391

Figure Lengend Snippet: Effect of CHK1 and ATM inhibition on fragile site expression at FRAXA in FX lymphoblastoid cells. ( A ) FX lymphoblastoid cells treated with UCN-01 with and without FdU. ( B ) FX lymphoblastoid cells treated with KU55933 with and without FdU. The data shows the average of three independent experiments for each cell line. Unt: untreated or vehicle only.

Article Snippet: Two male FX lymphoblastoid cells lines (GM03200B and GM07294), two normal lymphoblastoid cell lines (GM06895 and GM06865), an Ataxia Telangiectasia cell line (GM00719), a Seckel disease cell line (GM09703) and FXS fibroblasts (GM05848) were obtained from the Coriell Institute for Medical Research (Camden, NJ).

Techniques: Inhibition, Expressing

Journal: Nucleic Acids Research

Article Title: The role of DNA damage response pathways in chromosome fragility in Fragile X syndrome

doi: 10.1093/nar/gkp391

Figure Lengend Snippet: Colocalization of FX alleles with γ-H2AX foci in the presence of FdU. Immunocytochemistry combined with FISH was used to examine the colocalization of γ-H2AX foci and the FMR1 gene in normal (GM06865 and GM06895) and FXS lymphoblastoid cells (GM07294 and GM03200B) treated with or without 1 µM FdU for 18 h. ( A ) Representative images of γ-H2AX (green) and FMR1 (red) stained cells. ( B ) Proportion of cells in which γ-H2AX and FMR1 colocalize. The data shows the average of two independent experiments in which a 100 FMR1 signals were counted for each experiment. Error bars signify standard deviation. Statistical analysis was carried out using Fishers exact test. A single asterisk indicates a P -value <0.01, while a double asterisk indicates a P -value <0.005. Note that the X chromosome tends to be located at the nuclear periphery . This can make it seem in some cases as if the FMR1 signal is located outside of the nucleus.

Article Snippet: Two male FX lymphoblastoid cells lines (GM03200B and GM07294), two normal lymphoblastoid cell lines (GM06895 and GM06865), an Ataxia Telangiectasia cell line (GM00719), a Seckel disease cell line (GM09703) and FXS fibroblasts (GM05848) were obtained from the Coriell Institute for Medical Research (Camden, NJ).

Techniques: Immunocytochemistry, Staining, Standard Deviation