Journal: Molecular Medicine Reports

Article Title: Alterations in the long non-coding RNA transcriptome in mesangial cells treated with aldosterone in vitro

doi: 10.3892/mmr.2017.7313

Figure Lengend Snippet: Reverse transcription-quantitative polymerase chain reaction quantification of microarray hybridization. (A) The relative expression level of lncRNAs BC168211, BC088254, AF336872, AY325162, BC168687, AF230638 and BC167085. (B) The relative expression levels of upregulated mRNAs, NM_001108598, NM_001109190 and NM_001101018, and (C) downregulated mRNAs, NM_019347, NM_177,962 and NM_0,011,08823. *P<0.05 vs. the control group. ALD, aldosterone; lncRNAs, long non-coding RNAs; MCs, mesangial cells.

Article Snippet: The cells were stored at −80°C and sent to the LncRNA Expression Microarray (Arraystar, Rockville, MD, USA).

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Microarray, Hybridization, Expressing, Control

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: M 6 A -mediated lncRNA SCIRT stability promotes NSCLC progression through binding to SFPQ and activating the PI3K/Akt pathway

doi: 10.1007/s00018-025-05594-z

Figure Lengend Snippet: SCIRT m 6 A modification and expression levels were elevated in NSCLC. ( a ) Heatmap of differentially m 6 A-methylated lncRNAs. ( b, c ) Differentially m 6 A-methylated lncRNA-related mRNAs were analyzed by GO and the KEGG using TCGA data. ( d ) SCIRT, RP11-385J1.2, and SNHG9 m 6 A methylation levels in 10 paired NSCLC and adjacent noncancerous tissues. ( e ) Relative expression levels of SCIRT in 10 paired NSCLC and adjacent noncancerous tissues. ( f ) SCIRT expression levels of NSCLC (A549, H1299, and H358) and bronchial epithelial cell lines (BEAS-2B). Data are presented as mean ± SD from at least three independent experiments. ** P < 0.01, *** P < 0.001

Article Snippet: Arraystar m 6 A lncRNA epitranscriptomic microarrays were used to screen differentially m 6 A-methylated lncRNAs in NSCLC, and array figures were shown in Supplementary Fig. . A total of 39 differential metylated candidates were identified with 24 upregulated genes and 15 downregulated genes (Table ).

Techniques: Modification, Expressing, Methylation

Journal: bioRxiv

Article Title: The long non-coding RNA NEAT1 regulates the transcriptional landscape of cardiomyocytes

doi: 10.1101/2024.06.27.600932

Figure Lengend Snippet: (A) Experimental setup to detect the deregulated lncRNA in hearts subjected to HF, HF+AAV9-SERCA2a relative to Sham hearts (B) Microarray based profiling of RNA isolated from rat hearts subjected to Myocardial infarction and rescue by Serca2a gene therapy. (C) qRT-PCR of Neat1 from RNA isolated from rat ventricle after MI and MI+Serca2a gene therapy. (D) qRT-PCR for detecting the change in expression of ANP, β-MHC, Neat1, and Neat1.2 in primary neonatal rat ventricular myocytes (NRCM) challenged with 100 µM Isoproterenol for 48 h. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between both groups. (E) Neat1 expression levels detected by qRT-PCR and ejection fraction (EF%) throughout heart failure progression (TAC, transverse aortic constriction; n = 5–8). * p value < 0.05, t-tests were performed to compute statistical differences between Sham and TAC. (F) Neat1 expression levels and ejection fraction (EF%) throughout heart failure progression after subjecting mice to 15 mg/kg/day, 30mg/kg/day, and 50 mg/kg/day of isoproterenol for 10 days. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between saline and isoproterenol groups. (G) qRT-PCR for detecting the expression of total Neat1 and Neat1.2 in adult mice cardiomyocytes and adult mice non-myocyte fraction.

Article Snippet: Rat lncRNA microarray (4X44K, Arraystar) was performed in the heart samples isolated from the rats that were subjected to proximal coronary artery ligation (HF), HF+ SERCA2A gene therapy.

Techniques: Microarray, Isolation, Quantitative RT-PCR, Expressing, Saline

Journal: Aging (Albany NY)

Article Title: Calycosin stimulates the proliferation of endothelial cells, but not breast cancer cells, via a feedback loop involving RP11-65M17.3, BRIP1 and ERα

doi: 10.18632/aging.202641

Figure Lengend Snippet: The effects of calycosin treatment on lncRNA profiles in HUVECs.

Article Snippet: Labeled cDNA was subjected to hybridization using the Human lncRNA OneArray Plus microarray (Phalanx Biotech Group, Taiwan), followed by scanning using an Agilent scanner (Agilent Technologies, USA).

Techniques: