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    Jena Bioscience non hydrolyzable atp analogues
    Microtubule end residence time of Kip3. ( A ) End residence time in <t>ATP</t> ( black circles ) with exponential fits for hindering loads ( black dotted line ) and assisting loads > 0.5 pN ( black line ), run time on the MT lattice in ATP ( open red squares ) with exponential fit ( red line ), attachment time in <t>AMPPNP</t> ( open green hexagons ) with exponential fit ( green line ), attachment time in ADP ( open blue diamonds , ( 32 )) with exponential fit ( blue line ), and end residence time under sideward loads ( open black circle , ( 19 )). In the absence of load, mean end residence time ( grey circle ) and run time ( red square ) from TIRF measurements together with the literature value for the end residence time of truncated Kip3∆T-LZ ( purple circle , ( 14 )) are plotted. ( B ) Run length ( black circles ) of Kip3 as a function of load with exponential fit ( black line ) together with the zero-force run length measured in TIRF assays ( grey circle ) and run lengths from the literature ( 14 ) for truncated Kip3∆T-LZ ( purple circle ). From the original run length data that included slipping events, the average cumulative slip distance from ( 16 ) was subtracted and plotted as open black circles together with a model ( black dashed line , see text). All error bars are SEMs.
    Non Hydrolyzable Atp Analogues, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    non hydrolyzable atp analogues - by Bioz Stars, 2021-06
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    Microtubule end residence time of Kip3. ( A ) End residence time in ATP ( black circles ) with exponential fits for hindering loads ( black dotted line ) and assisting loads > 0.5 pN ( black line ), run time on the MT lattice in ATP ( open red squares ) with exponential fit ( red line ), attachment time in AMPPNP ( open green hexagons ) with exponential fit ( green line ), attachment time in ADP ( open blue diamonds , ( 32 )) with exponential fit ( blue line ), and end residence time under sideward loads ( open black circle , ( 19 )). In the absence of load, mean end residence time ( grey circle ) and run time ( red square ) from TIRF measurements together with the literature value for the end residence time of truncated Kip3∆T-LZ ( purple circle , ( 14 )) are plotted. ( B ) Run length ( black circles ) of Kip3 as a function of load with exponential fit ( black line ) together with the zero-force run length measured in TIRF assays ( grey circle ) and run lengths from the literature ( 14 ) for truncated Kip3∆T-LZ ( purple circle ). From the original run length data that included slipping events, the average cumulative slip distance from ( 16 ) was subtracted and plotted as open black circles together with a model ( black dashed line , see text). All error bars are SEMs.

    Journal: bioRxiv

    Article Title: The kinesin-8 Kip3 depolymerizes microtubules with a collective force-dependent mechanism

    doi: 10.1101/844829

    Figure Lengend Snippet: Microtubule end residence time of Kip3. ( A ) End residence time in ATP ( black circles ) with exponential fits for hindering loads ( black dotted line ) and assisting loads > 0.5 pN ( black line ), run time on the MT lattice in ATP ( open red squares ) with exponential fit ( red line ), attachment time in AMPPNP ( open green hexagons ) with exponential fit ( green line ), attachment time in ADP ( open blue diamonds , ( 32 )) with exponential fit ( blue line ), and end residence time under sideward loads ( open black circle , ( 19 )). In the absence of load, mean end residence time ( grey circle ) and run time ( red square ) from TIRF measurements together with the literature value for the end residence time of truncated Kip3∆T-LZ ( purple circle , ( 14 )) are plotted. ( B ) Run length ( black circles ) of Kip3 as a function of load with exponential fit ( black line ) together with the zero-force run length measured in TIRF assays ( grey circle ) and run lengths from the literature ( 14 ) for truncated Kip3∆T-LZ ( purple circle ). From the original run length data that included slipping events, the average cumulative slip distance from ( 16 ) was subtracted and plotted as open black circles together with a model ( black dashed line , see text). All error bars are SEMs.

    Article Snippet: In another control, we measured the attachment time of Kip3 (123 events of 17 microspheres) as a function of load in the presence of the non-hydrolyzable ATP analog adenosine-5’-[(β, γ )-imido] triphosphate (AMPPNP, Jena Bioscience).

    Techniques: