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Effects of IL‐17A neutralisation on macrophage polarisation in SHR renal tissues. (A) Representative IHC staining of M1 macrophage markers <t>(iNOS</t> and CD86) with quantitative analysis of their positive areas. (B) Representative IHC staining of M2 macrophage markers (Arg‐1 and CD163) with quantitative analysis of their positive areas. (C) Representative immunoblots and relative expression levels of iNOS, CD86, Arg‐1, and CD163 proteins. (D) Proportion of CD86 + and CD163 + cells among CD68 + macrophages. (E) mRNA expression levels of iNOS, CD86, Arg‐1, and CD163. Data are presented as mean ± SD ( n = 6).
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Effects of IL‐17A neutralisation on macrophage polarisation in SHR renal tissues. (A) Representative IHC staining of M1 macrophage markers <t>(iNOS</t> and CD86) with quantitative analysis of their positive areas. (B) Representative IHC staining of M2 macrophage markers (Arg‐1 and CD163) with quantitative analysis of their positive areas. (C) Representative immunoblots and relative expression levels of iNOS, CD86, Arg‐1, and CD163 proteins. (D) Proportion of CD86 + and CD163 + cells among CD68 + macrophages. (E) mRNA expression levels of iNOS, CD86, Arg‐1, and CD163. Data are presented as mean ± SD ( n = 6).
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Cusabio rat inducible nitric oxide synthase inos elisa kit
Fig. 13. Effects of increased concentrations (1–1000 nM, for 6 h) of anandamide (AEA), 2-arachidonoylglycerol (2-AG), CP 55–940, WIN 55,212–2, and URB597, on inducible-nitric oxide <t>synthase</t> <t>(iNOS;</t> A), Interleukin-1β (IL-1β; B), and Tumor Necrosis Factor-α (TNF-α; C) levels in primary hippocampal neurons exposed to high glucose (GLU; 150 mM for 48 h) + amyloid β peptide (Aβ1-42; 500 nM for 24 h). Data are mean values ± S.E.M. of n = 2 experiments per assay. **P ≤0.01 and ʄP ≤ 0.001, different from the control group; +P ≤0.05, ++P ≤0.01 and ɸP ≤0.001, different from the GLU + Aβ1-42 group; Kruskal-Wallis ANOVA with uncorrected Dunn’s test.
Rat Inducible Nitric Oxide Synthase Inos Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 13. Effects of increased concentrations (1–1000 nM, for 6 h) of anandamide (AEA), 2-arachidonoylglycerol (2-AG), CP 55–940, WIN 55,212–2, and URB597, on inducible-nitric oxide <t>synthase</t> <t>(iNOS;</t> A), Interleukin-1β (IL-1β; B), and Tumor Necrosis Factor-α (TNF-α; C) levels in primary hippocampal neurons exposed to high glucose (GLU; 150 mM for 48 h) + amyloid β peptide (Aβ1-42; 500 nM for 24 h). Data are mean values ± S.E.M. of n = 2 experiments per assay. **P ≤0.01 and ʄP ≤ 0.001, different from the control group; +P ≤0.05, ++P ≤0.01 and ɸP ≤0.001, different from the GLU + Aβ1-42 group; Kruskal-Wallis ANOVA with uncorrected Dunn’s test.
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Elabscience Biotechnology anti nitric oxide synthase 1
Fig. 13. Effects of increased concentrations (1–1000 nM, for 6 h) of anandamide (AEA), 2-arachidonoylglycerol (2-AG), CP 55–940, WIN 55,212–2, and URB597, on inducible-nitric oxide <t>synthase</t> <t>(iNOS;</t> A), Interleukin-1β (IL-1β; B), and Tumor Necrosis Factor-α (TNF-α; C) levels in primary hippocampal neurons exposed to high glucose (GLU; 150 mM for 48 h) + amyloid β peptide (Aβ1-42; 500 nM for 24 h). Data are mean values ± S.E.M. of n = 2 experiments per assay. **P ≤0.01 and ʄP ≤ 0.001, different from the control group; +P ≤0.05, ++P ≤0.01 and ɸP ≤0.001, different from the GLU + Aβ1-42 group; Kruskal-Wallis ANOVA with uncorrected Dunn’s test.
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Elabscience Biotechnology enos e el m0456 elisa assays
Fig. 13. Effects of increased concentrations (1–1000 nM, for 6 h) of anandamide (AEA), 2-arachidonoylglycerol (2-AG), CP 55–940, WIN 55,212–2, and URB597, on inducible-nitric oxide <t>synthase</t> <t>(iNOS;</t> A), Interleukin-1β (IL-1β; B), and Tumor Necrosis Factor-α (TNF-α; C) levels in primary hippocampal neurons exposed to high glucose (GLU; 150 mM for 48 h) + amyloid β peptide (Aβ1-42; 500 nM for 24 h). Data are mean values ± S.E.M. of n = 2 experiments per assay. **P ≤0.01 and ʄP ≤ 0.001, different from the control group; +P ≤0.05, ++P ≤0.01 and ɸP ≤0.001, different from the GLU + Aβ1-42 group; Kruskal-Wallis ANOVA with uncorrected Dunn’s test.
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Image Search Results


Effects of IL‐17A neutralisation on macrophage polarisation in SHR renal tissues. (A) Representative IHC staining of M1 macrophage markers (iNOS and CD86) with quantitative analysis of their positive areas. (B) Representative IHC staining of M2 macrophage markers (Arg‐1 and CD163) with quantitative analysis of their positive areas. (C) Representative immunoblots and relative expression levels of iNOS, CD86, Arg‐1, and CD163 proteins. (D) Proportion of CD86 + and CD163 + cells among CD68 + macrophages. (E) mRNA expression levels of iNOS, CD86, Arg‐1, and CD163. Data are presented as mean ± SD ( n = 6).

Journal: Clinical and Experimental Pharmacology & Physiology

Article Title: Mechanisms of IL‐17A Neutralisation in Alleviating Renal Fibrosis and Inflammation in Spontaneously Hypertensive Rats

doi: 10.1111/1440-1681.70116

Figure Lengend Snippet: Effects of IL‐17A neutralisation on macrophage polarisation in SHR renal tissues. (A) Representative IHC staining of M1 macrophage markers (iNOS and CD86) with quantitative analysis of their positive areas. (B) Representative IHC staining of M2 macrophage markers (Arg‐1 and CD163) with quantitative analysis of their positive areas. (C) Representative immunoblots and relative expression levels of iNOS, CD86, Arg‐1, and CD163 proteins. (D) Proportion of CD86 + and CD163 + cells among CD68 + macrophages. (E) mRNA expression levels of iNOS, CD86, Arg‐1, and CD163. Data are presented as mean ± SD ( n = 6).

Article Snippet: Sections were then incubated overnight at 4°C with primary antibodies against: E‐cadherin (Boster, China), Collagen III (Boster, China), inducible nitric oxide synthase (iNOS) (Boster, China), CD86 (Boster, China), arginase‐1 (Arg‐1) (Boster, China), CD163 (Boster, China), α‐smooth muscle actin (α‐SMA) (Cell Signalling Technology, USA).

Techniques: Immunohistochemistry, Western Blot, Expressing

Fig. 13. Effects of increased concentrations (1–1000 nM, for 6 h) of anandamide (AEA), 2-arachidonoylglycerol (2-AG), CP 55–940, WIN 55,212–2, and URB597, on inducible-nitric oxide synthase (iNOS; A), Interleukin-1β (IL-1β; B), and Tumor Necrosis Factor-α (TNF-α; C) levels in primary hippocampal neurons exposed to high glucose (GLU; 150 mM for 48 h) + amyloid β peptide (Aβ1-42; 500 nM for 24 h). Data are mean values ± S.E.M. of n = 2 experiments per assay. **P ≤0.01 and ʄP ≤ 0.001, different from the control group; +P ≤0.05, ++P ≤0.01 and ɸP ≤0.001, different from the GLU + Aβ1-42 group; Kruskal-Wallis ANOVA with uncorrected Dunn’s test.

Journal: Neurochemistry international

Article Title: Cannabinoid-profiled agents improve cell survival via reduction of oxidative stress and inflammation, and Nrf2 activation in a toxic model combining hyperglycemia+Aβ 1-42 peptide in rat hippocampal neurons.

doi: 10.1016/j.neuint.2020.104817

Figure Lengend Snippet: Fig. 13. Effects of increased concentrations (1–1000 nM, for 6 h) of anandamide (AEA), 2-arachidonoylglycerol (2-AG), CP 55–940, WIN 55,212–2, and URB597, on inducible-nitric oxide synthase (iNOS; A), Interleukin-1β (IL-1β; B), and Tumor Necrosis Factor-α (TNF-α; C) levels in primary hippocampal neurons exposed to high glucose (GLU; 150 mM for 48 h) + amyloid β peptide (Aβ1-42; 500 nM for 24 h). Data are mean values ± S.E.M. of n = 2 experiments per assay. **P ≤0.01 and ʄP ≤ 0.001, different from the control group; +P ≤0.05, ++P ≤0.01 and ɸP ≤0.001, different from the GLU + Aβ1-42 group; Kruskal-Wallis ANOVA with uncorrected Dunn’s test.

Article Snippet: Rat Inducible Nitric Oxide Synthase (iNOS) ELISA kit and Rat 3-nitrotyrosine (3-NT) ELISA kit were obtained from Cusabio Technology LLC (Houston, TX, USA).

Techniques: Control