nist Search Results


nist stool matrix  (ATCC)
93
ATCC nist stool matrix
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Nist Stool Matrix, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nist/NIST+Standard+Reference+Material+Genomic+DNA+Standards+for+Her2+Measurements%3BHuman/pmc08486790-90-0-15
Average 93 stars, based on 1 article reviews
nist stool matrix - by Bioz Stars, 2026-09
93/100 stars
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buffer solutions  (Thermo Fisher)
94
Thermo Fisher buffer solutions
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Buffer Solutions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nist/Buffer+solution+pH+4%2E01+(%2B%2F-0%2E022+%40+25oC)+No+Color+Specpure+NIST+Traceable/pm41816938-244-13-26
Average 94 stars, based on 1 article reviews
buffer solutions - by Bioz Stars, 2026-09
94/100 stars
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borate buffer  (Thermo Fisher)
94
Thermo Fisher borate buffer
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Borate Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nist/Buffer+solution+pH+9%2E2%2C+Borate+buffer%2C+traceable+to+NIST%2C+ready+to+use/bio_rxiv__2022__01__06__473910-216-9-11
Average 94 stars, based on 1 article reviews
borate buffer - by Bioz Stars, 2026-09
94/100 stars
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nist mass spectral library  (Revvity)
90
Revvity nist mass spectral library
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Nist Mass Spectral Library, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nist/NIST+2011+Mass+Spectral+Library+and+Software/pmc08199543-220-26-35
Average 90 stars, based on 1 article reviews
nist mass spectral library - by Bioz Stars, 2026-09
90/100 stars
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buffer h  (Thermo Fisher)
88
Thermo Fisher buffer h
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Buffer H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nist/Buffer+solution%2C+pH+8%2E00+(%2B%2F-0%2E024+%40+25oC)%2C+No+Color%2C+Specpure%2C+NIST+Traceable/pmc08527524-686-40-44
Average 88 stars, based on 1 article reviews
buffer h - by Bioz Stars, 2026-09
88/100 stars
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citric buffer ph7  (Thermo Fisher)
94
Thermo Fisher citric buffer ph7
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Citric Buffer Ph7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nist/Buffer+solution+pH+7%2E00+(%2B%2F-0%2E024+%40+25oC)+No+Color+Specpure+NIST+Traceable/us12643927-516-12-15
Average 94 stars, based on 1 article reviews
citric buffer ph7 - by Bioz Stars, 2026-09
94/100 stars
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hepes  (Chem Impex International)
95
Chem Impex International hepes
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Hepes, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nist/4-(2-Hydroxyethyl)-1-piperazineethanesulfonic+acid/pm38779885-317-29-30
Average 95 stars, based on 1 article reviews
hepes - by Bioz Stars, 2026-09
95/100 stars
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technology nist  (ATCC)
91
ATCC technology nist
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Technology Nist, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nist/NIST+Standard+Reference+Material+Human+DNA+Quantitation+Standard%3BHuman/pmc09821876-233-38-41
Average 91 stars, based on 1 article reviews
technology nist - by Bioz Stars, 2026-09
91/100 stars
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hier buffer h  (Thermo Fisher)
93
Thermo Fisher hier buffer h
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Hier Buffer H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nist/Buffer+solution%2C+pH+9%2E00+(%2B%2F-0%2E024+%40+25oC)%2C+No+Color%2C+Specpure%2C+NIST+Traceable/pm40259333-104-16-21
Average 93 stars, based on 1 article reviews
hier buffer h - by Bioz Stars, 2026-09
93/100 stars
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gm12878 human lymphocytes  (ATCC)
94
ATCC gm12878 human lymphocytes
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Gm12878 Human Lymphocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nist/SRM+Human+DNA+for+Whole-Genome+Variant+Assessment(Daughter+of+Utah%2FEuropean+Ancestry)%3BHuman/pmc11252913__pnas__2403130121__sapp-10-0-3
Average 94 stars, based on 1 article reviews
gm12878 human lymphocytes - by Bioz Stars, 2026-09
94/100 stars
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buffer solutions 4  (Thermo Fisher)
92
Thermo Fisher buffer solutions 4
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Buffer Solutions 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nist/Buffer+solution+pH+4%2C+Phthalate+buffer%2C+traceable+to+NIST%2C+ready+to+use/10__30997_slash_ijar__v5i2__498-37-59-54
Average 92 stars, based on 1 article reviews
buffer solutions 4 - by Bioz Stars, 2026-09
92/100 stars
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buffer a  (Thermo Fisher)
91
Thermo Fisher buffer a
Stool samples collected from omnivorous donors and processed into a single standardized matrix by <t>NIST</t> was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 <t>3</t> <t>(triangle)</t> or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.
Buffer A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nist/Buffer+solution%2C+pH+11%2E00+(%2B%2F-0%2E026+%40+25oC)%2C+No+Color%2C+Specpure%2C+NIST+Traceable/10__1016_slash_j__aqrep__2023__101550-76-6-34
Average 91 stars, based on 1 article reviews
buffer a - by Bioz Stars, 2026-09
91/100 stars
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Image Search Results


Stool samples collected from omnivorous donors and processed into a single standardized matrix by NIST was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 3 (triangle) or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.

Journal: Nature Communications

Article Title: Standardized preservation, extraction and quantification techniques for detection of fecal SARS-CoV-2 RNA

doi: 10.1038/s41467-021-25576-6

Figure Lengend Snippet: Stool samples collected from omnivorous donors and processed into a single standardized matrix by NIST was spiked with ATCC CoV-2 RNA or BCoV vaccine. Spiked stool was preserved in the OMNIgene-GUT Kit (OG), Zymo DNA/RNA shield buffer (ZY), and PBS (as indicated in the tab on the top). RNA was extracted from these samples by two independent users, each in duplicate, using the MagMAX Viral/Pathogen Kit (MM; green), QIAamp Viral RNA Mini Kit (QA; orange), or Zymo Quick-RNA Viral Kit (ZY; purple) as indicated on the x -axis. RNA was assayed using ddPCR. a Absolute concentration of SARS-CoV-2 RNA assayed by ddPCR targeting the N1 gene is plotted on the y -axis. NIST stool matrix was spiked with 10 3 (triangle) or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis. NIST stool matrix was spiked with 1:10 diluted (triangle) or undiluted (square) BCoV vaccine. Control samples with no spiked in RNA (none; circle) were included in duplicate to estimate LoB. U stands for undetermined and marks samples with no detectable RNA above LoB. Two-sided paired T tests were performed on n = 4 independent extractions for each spike-in condition. Associated statistics are summarized in Supplementary Data . Source data are provided as a Source data file.

Article Snippet: NIST stool matrix was spiked with 10 3 (triangle) or 10 4 (square) copies of ATCC synthetic SARS-CoV-2 RNA. b Absolute concentration of BCoV RNA assayed by ddPCR targeting the M gene is plotted on the y -axis.

Techniques: Concentration Assay, Control