Journal: Applied Spectroscopy Reviews

Article Title: The use of vibrational spectroscopy to study the pathogenesis multiple sclerosis and other neurological conditions

doi: 10.1080/05704928.2017.1336450

Figure Lengend Snippet: Figure 1. Mean FTIR spectra for four post-mortem samples analyzed in the preliminary study: chronic lesion (blue), active lesion (red), NAWM (green) and control (black). All samples were subjected to FTIR analysis at the Focas Research Institute, DIT, using a Perkin Elmer Spotlight 400N FTIR imaging system, incorporating a liquid nitrogen cooled mercury cadmium telluride 16 £ 1, 6.25-mm pixel array detector, and acquired by the Spectral Image software. FTIR images from the tissue sections (10 mm sections) mounted on CaF2 slides were recorded over the range 4000–800 cm¡1 in transmittance mode with a reso- lution of 4 cm¡1 and interferometer speed of 1.0 cm¡1/second at continuously varying magnification. The scans per pixel for background were 120 and, for images, 16 per pixel, respectively. Spectroscopic data analysis was carried out in Matlab, version R2013 (Mathworks, CA, USA) according to protocols developed and routinely used in-house at DIT.

Article Snippet: All samples were subjected to FTIR analysis at the Focas Research Institute, DIT, using a Perkin Elmer Spotlight 400N FTIR imaging system, incorporating a liquid nitrogen cooled mercury cadmium telluride 16 £ 1, 6.25-mm pixel array detector, and acquired by the Spectral Image software.

Techniques: Control, Imaging, Software

Journal: Applied Spectroscopy Reviews

Article Title: The use of vibrational spectroscopy to study the pathogenesis multiple sclerosis and other neurological conditions

doi: 10.1080/05704928.2017.1336450

Figure Lengend Snippet: Figure 2. PCA of the four post-mortem samples FTIR data. (A) 2-D PCA scatterplot showing a separation between chronic (blue) and active lesion (red) and, NAWM (green) and control (black). (B) PC1 loading, responsible for the separation, is negatively dominated by peaks assigned to lipids around 2800– 3000 cm¡1.

Article Snippet: All samples were subjected to FTIR analysis at the Focas Research Institute, DIT, using a Perkin Elmer Spotlight 400N FTIR imaging system, incorporating a liquid nitrogen cooled mercury cadmium telluride 16 £ 1, 6.25-mm pixel array detector, and acquired by the Spectral Image software.

Techniques: Control

Journal: Applied Spectroscopy Reviews

Article Title: The use of vibrational spectroscopy to study the pathogenesis multiple sclerosis and other neurological conditions

doi: 10.1080/05704928.2017.1336450

Figure Lengend Snippet: Figure 3. PCA of the NAWM and control FTIR data. (A) The 2-D PCA plot shows a separation between NAWM (black) and control (green) FTIR spectra in PC1 which explains 80.63% of the variation found in the data. (B) The PC1 loading is negatively dominated by peaks assigned to lipids around 2800–3000 cm¡1.

Article Snippet: All samples were subjected to FTIR analysis at the Focas Research Institute, DIT, using a Perkin Elmer Spotlight 400N FTIR imaging system, incorporating a liquid nitrogen cooled mercury cadmium telluride 16 £ 1, 6.25-mm pixel array detector, and acquired by the Spectral Image software.

Techniques: Control

Journal: Journal of neurosurgery

Article Title: Folate receptor overexpression can be visualized in real time during pituitary adenoma endoscopic transsphenoidal surgery with near-infrared imaging

doi: 10.3171/2017.2.JNS163191

Figure Lengend Snippet: Visionsense equipment setup for NIR imaging. Upper: The excitation laser for NIR imaging is attached to a 4-mm endoscope. Copyright Visionsense. Published with permission. Lower: Images are displayed on the leftmost screen.

Article Snippet: Visionsense equipment setup for NIR imaging.

Techniques: Imaging

Journal: Journal of neurosurgery

Article Title: Folate receptor overexpression can be visualized in real time during pituitary adenoma endoscopic transsphenoidal surgery with near-infrared imaging

doi: 10.3171/2017.2.JNS163191

Figure Lengend Snippet: Case 7. This patient had an NF adenoma, high FRα expression on final pathological analysis, and intraoperative NIR fluorescence. A and B: Preoperative sagittal (A) and coronal (B) MR images without contrast showing a 17-mm mass in the sella. C and D: Intraoperative endoscopic imaging with the dura open shown with white light only (C) and the NIR signal superimposed on white light (D). The MOCRs can be seen clearly and are used as a measure of distance from the endoscope to the target. E and F: Intraoperative endoscopic image of the sella after resection of the gross tumor. The residual tumor was identified on the right, both with white light only (E) and the NIR signal superimposed on the white light (F). This fluorescent specimen was confirmed on the final pathological analysis to be consistent with pituitary adenoma. G and H: Intraoperative endoscopic image of sella contents after resection of the residual neoplasm with white light only (G) and NIR superimposed on white light (H). No obvious fluorescence is identified. I: Example of SBR and MOCR calculation. Five points are chosen in the gross tumor itself, with the Visionsense camera calculating NIR signal for the points (75 + 74 + 66 + 61 + 57)/5 = 67 ± 7.9. Five points are chosen in the surrounding bone close to the tumor but outside of the obvious specimen (22 + 29 + 34 + 19 + 17)/5 = 24 ± 7.1. The SBR is thus 67/24 = 2.8. The black triangles denote the locations of the MOCRs, spanning roughly 50% of the field, that were used to approximate the endoscope distance.

Article Snippet: Visionsense equipment setup for NIR imaging.

Techniques: Expressing, Fluorescence, Imaging

Journal: Pharmaceuticals

Article Title: Fluorochrome Selection for Imaging Intraoperative Ovarian Cancer Probes

doi: 10.3390/ph15060668

Figure Lengend Snippet: Organ biodistribution and intraoperative NIR FLI; ( A ) “Ex vivo” NIR FLI 6 h after 500 µM injection of RR11 and 23 ( MSA14 ) was performed to assess the compound accumulation in tumors and organs. ( B ) Representative intraoperative near-infrared (NIR), color and pseudo-colored fluorescent signal merge images of one 23 ( MSA14 ) injected subcutaneously engrafted OVCAR-3 mouse in vivo and ex vivo.

Article Snippet: A second intraoperative compatible imaging system (FLARE ® , Curadel LLC, Nattick, MA, USA) was used to assess the clinical utility of 23 ( MSA14 ).

Techniques: Ex Vivo, Injection, In Vivo

Journal: Pharmaceuticals

Article Title: Fluorochrome Selection for Imaging Intraoperative Ovarian Cancer Probes

doi: 10.3390/ph15060668

Figure Lengend Snippet: Biodistribution of RR11 and 23 ( MSA14 ) in SKOV-3 s.c. models. RR11 ( A ) and 23 ( MSA14 ) ( B ) were intravenously injected into n = 2 and n = 4 mice, respectively, and imaged at 2, 6, and 24 h with a dose of 500 and 100 µM. The fluorescence signal in the tumors (*), liver (#), limb (†), spine (‡) and background in the flank muscle (yellow square), is presented in one representative mouse. All animals were euthanized when indicated and “ex vivo” NIR FLI was performed. ( C ) 23 (MSA14) injected SKOV-3 mice were additionally imaged with the intraoperative imaging system “in vivo” and “ex vivo”. NIR exposure times are indicated in brackets.

Article Snippet: A second intraoperative compatible imaging system (FLARE ® , Curadel LLC, Nattick, MA, USA) was used to assess the clinical utility of 23 ( MSA14 ).

Techniques: Injection, Fluorescence, Ex Vivo, Imaging, In Vivo

Journal: Translational Medicine @ UniSa

Article Title: Optical Tecnology Developments in Biomedicine: History, Current and Future

doi:

Figure Lengend Snippet: A photograph of the whole apparatus (a) illustrates the handheld puck or probe, the coupling to the circuit box which contains the drivers for the LED, the amplifiers for the detectors, the digitally controlled gain adjustment amplifier, the electronic switch which decodes the light pulses and stores the information in a memory capacitor, the second set of switches which sample the memory capacitor at a rate compatible with the computer analog-to-digital converter (ADC), and (b) Handheld puck. From Ref. , with permission.

Article Snippet: In another pilot clinical trial of 48 patients, Xu et al used a portable handheld NIR imaging device, “P-Scan”, a pre-commercial prototype of ViOptix Inc. (Fremont, CA, USA), to image suspicious breast lesions identified on diagnostic clinical ultrasound (US) [ ].

Techniques:

Journal: Molecular Vision

Article Title: Genetic and environmental risk factors for reticular pseudodrusen in the EUGENDA study

doi:

Figure Lengend Snippet: Imaging of reticular pseudodrusen. Example of reticular pseudodrusen (RPD) visible via fundus autofluorescence ( A ), infrared imaging ( B ) and spectral-domain optical coherence tomography (SD-OCT; C ).

Article Snippet: Retinal images, including SD-OCT volume scans registered over NIR images (Spectralis SDOCT, Heidelberg Engineering, Heidelberg, Germany) and stereo color FP (FP, Cologne: Canon UVI fundus camera using 40° field of view; Canon, Tokyo, Japan, and Nijmegen: Topcon TRC 50IX fundus camera using 50° field of view; Topcon, Tokyo, Japan), were collected from each participant.

Techniques: Imaging, Tomography