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A. Experimental approach for chemogenetic activation of SFOGLUT neurons (top left) while imaging InsCtx (top right). Bottom left: image of SFO showing <t>hM3Dq-mCherry</t> expression. Scale bar: 200 μm. Bottom right: two-photon image of InsCtx during SFOGLUT activation. Scale bar: 100 μm.
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Image Search Results


Key Resources Table

Journal: Neuron

Article Title: Estimation of Current and Future Physiological States in Insular Cortex

doi: 10.1016/j.neuron.2019.12.027

Figure Lengend Snippet: Key Resources Table

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Invitrogen Cat #: A10262; RRID: AB_2534023 Rat anti-mCherry Invitrogen Cat #: {"type":"entrez-nucleotide","attrs":{"text":"M11217","term_id":"333925"}} M11217 ; RRID: AB_2536611 Bacterial and Virus Strains AAV1-hSyn-GCaMP6f Penn Vector Core Addgene 100837 AAV1-hSyn-GCaMP6s Penn Vector Core Addgene 100843 AAV8-hSyn-DIO-hM3Dq-mCherry UNC Vector Core Addgene 44361 AAV8-hSyn-DIO-hM4Di-mCherry UNC Vector Core Addgene 44362 AAV1-DIO-ChR2(H134R)-YFP Penn Vector Core Addgene 20298 AAV8-FLEX-TVA-mCherry UNC Vector Core Addgene 38044 SADΔG–EGFP (EnvA) rabies Salk Gene Transfer Targeting and Therapeutics Core Addgene 32635 AAV8-CaMKII-hM3Dq-mCherry Addgene Addgene 50476 Chemicals, Peptides, and Recombinant Proteins Clozapine-N-Oxide (CNO) NIMH Drug Supply Program Cat# C-929 Compound 21 (C21) Tocris 6422 CNQX Tocris Cat# 0190 D-AP5 Tocris Cat# 0106 Kynurenic Acid Sigma-Aldrich Cat# K3375–5G Picrotoxin Tocris Cat# 1128 Bicuculline Tocris Cat# 2503 TTX Tocris Cat# 1069 4-AP Sigma-Aldrich Cat# A78403 Cholera Toxin B Subunit B (Recombinant), Alexa Fluor 555 Conjugate Thermo Fisher Cat# 34776 Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: Nos1-Cre knockin (B6.129-Nos1tm1(cre)Mgmj/J) The Jackson Laboratory Strain# 017526; RRID: IMSR_JAX:017526 Mouse: vGlut2-ires-Cre (B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ) The Jackson Laboratory Strain# 028863; RRID:IMSR_JAX:028863 Software and Algorithms MATLAB R2015b, 2019a Mathworks https://www.mathworks.com/products/matlab.html ; RRID: SCR_001622 MonkeyLogic Asaad and Eskandar, 2008 http://www.brown.edu/Research/monkeylogic/ MonkeyLogic2 NIMH https://monkeylogic.nimh.nih.gov/ Scanbox Neurolabware https://scanbox.org/ PCA/ICA neuron identification Mukamel et al., 2009 https://github.com/mukamel-lab/CellSort Glmnet Friedman et al., 2010 https://web.stanford.edu/~hastie/glmnet_matlab/ ImageJ 1.49t NIH https://imagej.nih.gov/ij/ ; RRID: SCR_003070 Open in a separate window Key Resources Table InsCtx ongoing activity patterns reflect current bodily state, not behavioral state Hypothalamic hunger/thirst neurons gate InsCtx responses to food/water cues Hypothalamic hunger/thirst neurons do not gate InsCtx ongoing activity Food/water cues drive transient “simulations” of future bodily states in InsCtx

Techniques: Virus, Plasmid Preparation, Recombinant, Knock-In, Software

A. Experimental approach for chemogenetic activation of SFOGLUT neurons (top left) while imaging InsCtx (top right). Bottom left: image of SFO showing hM3Dq-mCherry expression. Scale bar: 200 μm. Bottom right: two-photon image of InsCtx during SFOGLUT activation. Scale bar: 100 μm.

Journal: Neuron

Article Title: Estimation of Current and Future Physiological States in Insular Cortex

doi: 10.1016/j.neuron.2019.12.027

Figure Lengend Snippet: A. Experimental approach for chemogenetic activation of SFOGLUT neurons (top left) while imaging InsCtx (top right). Bottom left: image of SFO showing hM3Dq-mCherry expression. Scale bar: 200 μm. Bottom right: two-photon image of InsCtx during SFOGLUT activation. Scale bar: 100 μm.

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Invitrogen Cat #: A10262; RRID: AB_2534023 Rat anti-mCherry Invitrogen Cat #: {"type":"entrez-nucleotide","attrs":{"text":"M11217","term_id":"333925"}} M11217 ; RRID: AB_2536611 Bacterial and Virus Strains AAV1-hSyn-GCaMP6f Penn Vector Core Addgene 100837 AAV1-hSyn-GCaMP6s Penn Vector Core Addgene 100843 AAV8-hSyn-DIO-hM3Dq-mCherry UNC Vector Core Addgene 44361 AAV8-hSyn-DIO-hM4Di-mCherry UNC Vector Core Addgene 44362 AAV1-DIO-ChR2(H134R)-YFP Penn Vector Core Addgene 20298 AAV8-FLEX-TVA-mCherry UNC Vector Core Addgene 38044 SADΔG–EGFP (EnvA) rabies Salk Gene Transfer Targeting and Therapeutics Core Addgene 32635 AAV8-CaMKII-hM3Dq-mCherry Addgene Addgene 50476 Chemicals, Peptides, and Recombinant Proteins Clozapine-N-Oxide (CNO) NIMH Drug Supply Program Cat# C-929 Compound 21 (C21) Tocris 6422 CNQX Tocris Cat# 0190 D-AP5 Tocris Cat# 0106 Kynurenic Acid Sigma-Aldrich Cat# K3375–5G Picrotoxin Tocris Cat# 1128 Bicuculline Tocris Cat# 2503 TTX Tocris Cat# 1069 4-AP Sigma-Aldrich Cat# A78403 Cholera Toxin B Subunit B (Recombinant), Alexa Fluor 555 Conjugate Thermo Fisher Cat# 34776 Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: Nos1-Cre knockin (B6.129-Nos1tm1(cre)Mgmj/J) The Jackson Laboratory Strain# 017526; RRID: IMSR_JAX:017526 Mouse: vGlut2-ires-Cre (B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ) The Jackson Laboratory Strain# 028863; RRID:IMSR_JAX:028863 Software and Algorithms MATLAB R2015b, 2019a Mathworks https://www.mathworks.com/products/matlab.html ; RRID: SCR_001622 MonkeyLogic Asaad and Eskandar, 2008 http://www.brown.edu/Research/monkeylogic/ MonkeyLogic2 NIMH https://monkeylogic.nimh.nih.gov/ Scanbox Neurolabware https://scanbox.org/ PCA/ICA neuron identification Mukamel et al., 2009 https://github.com/mukamel-lab/CellSort Glmnet Friedman et al., 2010 https://web.stanford.edu/~hastie/glmnet_matlab/ ImageJ 1.49t NIH https://imagej.nih.gov/ij/ ; RRID: SCR_003070 Open in a separate window Key Resources Table InsCtx ongoing activity patterns reflect current bodily state, not behavioral state Hypothalamic hunger/thirst neurons gate InsCtx responses to food/water cues Hypothalamic hunger/thirst neurons do not gate InsCtx ongoing activity Food/water cues drive transient “simulations” of future bodily states in InsCtx

Techniques: Activation Assay, Imaging, Expressing

Key Resources Table

Journal: Neuron

Article Title: Estimation of Current and Future Physiological States in Insular Cortex

doi: 10.1016/j.neuron.2019.12.027

Figure Lengend Snippet: Key Resources Table

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Invitrogen Cat #: A10262; RRID: AB_2534023 Rat anti-mCherry Invitrogen Cat #: {"type":"entrez-nucleotide","attrs":{"text":"M11217","term_id":"333925"}} M11217 ; RRID: AB_2536611 Bacterial and Virus Strains AAV1-hSyn-GCaMP6f Penn Vector Core Addgene 100837 AAV1-hSyn-GCaMP6s Penn Vector Core Addgene 100843 AAV8-hSyn-DIO-hM3Dq-mCherry UNC Vector Core Addgene 44361 AAV8-hSyn-DIO-hM4Di-mCherry UNC Vector Core Addgene 44362 AAV1-DIO-ChR2(H134R)-YFP Penn Vector Core Addgene 20298 AAV8-FLEX-TVA-mCherry UNC Vector Core Addgene 38044 SADΔG–EGFP (EnvA) rabies Salk Gene Transfer Targeting and Therapeutics Core Addgene 32635 AAV8-CaMKII-hM3Dq-mCherry Addgene Addgene 50476 Chemicals, Peptides, and Recombinant Proteins Clozapine-N-Oxide (CNO) NIMH Drug Supply Program Cat# C-929 Compound 21 (C21) Tocris 6422 CNQX Tocris Cat# 0190 D-AP5 Tocris Cat# 0106 Kynurenic Acid Sigma-Aldrich Cat# K3375–5G Picrotoxin Tocris Cat# 1128 Bicuculline Tocris Cat# 2503 TTX Tocris Cat# 1069 4-AP Sigma-Aldrich Cat# A78403 Cholera Toxin B Subunit B (Recombinant), Alexa Fluor 555 Conjugate Thermo Fisher Cat# 34776 Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: Nos1-Cre knockin (B6.129-Nos1tm1(cre)Mgmj/J) The Jackson Laboratory Strain# 017526; RRID: IMSR_JAX:017526 Mouse: vGlut2-ires-Cre (B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ) The Jackson Laboratory Strain# 028863; RRID:IMSR_JAX:028863 Software and Algorithms MATLAB R2015b, 2019a Mathworks https://www.mathworks.com/products/matlab.html ; RRID: SCR_001622 MonkeyLogic Asaad and Eskandar, 2008 http://www.brown.edu/Research/monkeylogic/ MonkeyLogic2 NIMH https://monkeylogic.nimh.nih.gov/ Scanbox Neurolabware https://scanbox.org/ PCA/ICA neuron identification Mukamel et al., 2009 https://github.com/mukamel-lab/CellSort Glmnet Friedman et al., 2010 https://web.stanford.edu/~hastie/glmnet_matlab/ ImageJ 1.49t NIH https://imagej.nih.gov/ij/ ; RRID: SCR_003070 Open in a separate window Key Resources Table InsCtx ongoing activity patterns reflect current bodily state, not behavioral state Hypothalamic hunger/thirst neurons gate InsCtx responses to food/water cues Hypothalamic hunger/thirst neurons do not gate InsCtx ongoing activity Food/water cues drive transient “simulations” of future bodily states in InsCtx

Techniques: Plasmid Preparation, Recombinant, Knock-In, Software

Key Resources Table

Journal: Neuron

Article Title: Estimation of Current and Future Physiological States in Insular Cortex

doi: 10.1016/j.neuron.2019.12.027

Figure Lengend Snippet: Key Resources Table

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Invitrogen Cat #: A10262; RRID: AB_2534023 Rat anti-mCherry Invitrogen Cat #: {"type":"entrez-nucleotide","attrs":{"text":"M11217","term_id":"333925"}} M11217 ; RRID: AB_2536611 Bacterial and Virus Strains AAV1-hSyn-GCaMP6f Penn Vector Core Addgene 100837 AAV1-hSyn-GCaMP6s Penn Vector Core Addgene 100843 AAV8-hSyn-DIO-hM3Dq-mCherry UNC Vector Core Addgene 44361 AAV8-hSyn-DIO-hM4Di-mCherry UNC Vector Core Addgene 44362 AAV1-DIO-ChR2(H134R)-YFP Penn Vector Core Addgene 20298 AAV8-FLEX-TVA-mCherry UNC Vector Core Addgene 38044 SADΔG–EGFP (EnvA) rabies Salk Gene Transfer Targeting and Therapeutics Core Addgene 32635 AAV8-CaMKII-hM3Dq-mCherry Addgene Addgene 50476 Chemicals, Peptides, and Recombinant Proteins Clozapine-N-Oxide (CNO) NIMH Drug Supply Program Cat# C-929 Compound 21 (C21) Tocris 6422 CNQX Tocris Cat# 0190 D-AP5 Tocris Cat# 0106 Kynurenic Acid Sigma-Aldrich Cat# K3375–5G Picrotoxin Tocris Cat# 1128 Bicuculline Tocris Cat# 2503 TTX Tocris Cat# 1069 4-AP Sigma-Aldrich Cat# A78403 Cholera Toxin B Subunit B (Recombinant), Alexa Fluor 555 Conjugate Thermo Fisher Cat# 34776 Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: Nos1-Cre knockin (B6.129-Nos1tm1(cre)Mgmj/J) The Jackson Laboratory Strain# 017526; RRID: IMSR_JAX:017526 Mouse: vGlut2-ires-Cre (B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ) The Jackson Laboratory Strain# 028863; RRID:IMSR_JAX:028863 Software and Algorithms MATLAB R2015b, 2019a Mathworks https://www.mathworks.com/products/matlab.html ; RRID: SCR_001622 MonkeyLogic Asaad and Eskandar, 2008 http://www.brown.edu/Research/monkeylogic/ MonkeyLogic2 NIMH https://monkeylogic.nimh.nih.gov/ Scanbox Neurolabware https://scanbox.org/ PCA/ICA neuron identification Mukamel et al., 2009 https://github.com/mukamel-lab/CellSort Glmnet Friedman et al., 2010 https://web.stanford.edu/~hastie/glmnet_matlab/ ImageJ 1.49t NIH https://imagej.nih.gov/ij/ ; RRID: SCR_003070 Open in a separate window Key Resources Table InsCtx ongoing activity patterns reflect current bodily state, not behavioral state Hypothalamic hunger/thirst neurons gate InsCtx responses to food/water cues Hypothalamic hunger/thirst neurons do not gate InsCtx ongoing activity Food/water cues drive transient “simulations” of future bodily states in InsCtx

Techniques: Plasmid Preparation, Recombinant, Knock-In, Software