Journal: Journal of Biological Research

Article Title: Cortical microtubule orientation in Arabidopsis thaliana root meristematic zone depends on cell division and requires severing by katanin

doi: 10.1186/s40709-018-0082-6

Figure Lengend Snippet: Cortical microtubule orientation in the meristematic zone of untreated ( a ) and aphidicolin-treated ( b – d ) roots. Single CLSM sections through the boundary between protoderm and cortex are presented, so that in the center of each figure cortex cells can be observed. In all the images of this work the root tip is oriented towards the bottom of the page. In control root ( a ) prominent transverse orientation of cortical microtubules can be observed in interphase cells (arrowheads). At 12 h of treatment ( b ) transverse microtubule orientation persists (arrowheads), while dividing cells still exist (arrow points to a mitotic cell). After 24 h of treatment ( c ), cell divisions have ceased and cortical microtubules exhibit random orientation (cells within the brackets are cortex cells viewed at external cortical plane), which is also observed after 48 h of treatment ( d ; cells within brackets). In the latter image, vacuolation of meristematic cells in also prominent (asterisks show vacuoles). Representative decipher-graphs of microtubule alignment in meristematic cells of control ( e ), 12 h ( f ), 24 h ( g ) and 48 h ( h ) aphidicolin treatments. Decipher-graphs show that microtubules are transversely oriented (doubled-headed arrow) in the control and after 12 h of aphidicolin treatment ( e , f ), while the frequency of longitudinal and randomly oriented microtubules increased upon prolonged treatments ( g , h , respectively). Scale bar 10 μm

Article Snippet: The preparations were examined with a Nikon D-Eclipse C1 or a Zeiss LSM780 confocal laser scanning microscope (CLSM), with the appropriate filters for FITC, and micrographs were acquired with each manufacturer’s software.

Techniques: Control

Journal: Journal of Biological Research

Article Title: Cortical microtubule orientation in Arabidopsis thaliana root meristematic zone depends on cell division and requires severing by katanin

doi: 10.1186/s40709-018-0082-6

Figure Lengend Snippet: Cortical microtubule orientation at the external face of epidermal cells in the transition (i.e. under the shootward part of the lateral root cap; a , c ) and fast elongation ( b , d ) zones of aphidicolin-treated roots. All images are maximum projections of serial CLSM sections. After 24 h of treatment, microtubules are transverse (arrowheads) in the transition ( a ) and fast elongation ( b ) zone, while after 48 h of treatment they appear randomly oriented in both zones ( c , d ). Representative decipher-graphs of microtubule alignment in transition and elongation zone cells of control roots ( e ), and after 24 h ( f ) and 48 h ( g ) of aphidicolin treatment. Decipher-graphs show that microtubules are transversely oriented (doubled-headed arrow) in the control and after 24 h of aphidicolin treatment ( e and f , respectively), while the frequency of longitudinal and/or randomly oriented microtubules increased after 48 h of treatment ( g ). Scale bar 10 μm

Article Snippet: The preparations were examined with a Nikon D-Eclipse C1 or a Zeiss LSM780 confocal laser scanning microscope (CLSM), with the appropriate filters for FITC, and micrographs were acquired with each manufacturer’s software.

Techniques: Control

Journal: Journal of Biological Research

Article Title: Cortical microtubule orientation in Arabidopsis thaliana root meristematic zone depends on cell division and requires severing by katanin

doi: 10.1186/s40709-018-0082-6

Figure Lengend Snippet: Cortical microtubule orientation in cortex cells of wild-type ( a , e ) and p60-katanin mutants ( b – d , f – h ). All high magnification images ( e – h ) are maximum projections of serial CLSM sections through the cortical cytoplasm. Cortex cell files, derived from the areas defined by rectangular frames on low magnification images ( a – d ), were released after application of gentle pressure on the whole mount root specimens. Fairly transverse cortical microtubules can be observed in Col-0 cells ( e ). In ktn1 - 2 ( f ), lue1 ( g ) and fra2 ( h ), though a general transverse pattern exists, several microtubules exhibit random orientation, while foci of microtubule convergence can be discerned as well. Representative decipher-graphs of microtubule alignment in meristematic cortex cells of Col-0 ( i ), ktn1 - 2 ( j ), lue1 ( k ) and fra2 ( l ). Generally, transverse microtubule orientation prevailed in all the cases (doubled-headed arrow). However, in the katanin mutants an increase in the frequency of longitudinal and/or randomly oriented microtubules was noticed. Scale bars 50 μm ( a – d ), 10 μm ( e – h )

Article Snippet: The preparations were examined with a Nikon D-Eclipse C1 or a Zeiss LSM780 confocal laser scanning microscope (CLSM), with the appropriate filters for FITC, and micrographs were acquired with each manufacturer’s software.

Techniques: Derivative Assay, Gentle