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Image Search Results
Journal: PLoS ONE
Article Title: Rectified Cell Migration on Saw-Like Micro-Elastically Patterned Hydrogels with Asymmetric Gradient Ratchet Teeth
doi: 10.1371/journal.pone.0078067
Figure Lengend Snippet: The conditions used to prepare the gels are described in . (a) Phase contrast microscopy image of the gel. Sale bar: 100 µm. (b) Distribution of Young’s modulus in a single unit of the gel. Unit size: 90 µm. Elasticity peak: ca. 500 kPa. (c) Trajectories of 3T3 fibroblasts cultured on gels with PSLs. Cells were observed by time-lapse microscopy for 24 hr and images were acquired at 15 min intervals. (d) Time-course of ensemble averaged trajectories with standard deviation in the presence (red) and absence (blue) of PSLs. n=30 cells.
Article Snippet: The
Techniques: Microscopy, Cell Culture, Time-lapse Microscopy, Standard Deviation
Journal: PLoS ONE
Article Title: Circadian Disruption Accelerates Tumor Growth and Angio/Stromagenesis through a Wnt Signaling Pathway
doi: 10.1371/journal.pone.0015330
Figure Lengend Snippet: (A) 8-hydroxydeoxyguanosine levels are significantly increased in the lung tissues of L/L compared with L/D mice (* P <0.05). (B) Reporter assays. The promoter activity of the WNT10A gene was measured using a luciferase system after the addition of hydrogen peroxide. 42 hours after transfection (exposure time 6 hr) or 36 hours after transfection (exposure time 12 hr) of the reporter plasmid into PC3 cells, cells were treated with 10 µM of hydrogen peroxide. Luciferase activities were assayed after 48 hours of transfection. The results shown are normalized against protein concentrations measured using the Bradford method and are representative of at least three independent experiments. (C) Induction of mouse Wnt10a transcripts by oxidative stress. NIH3T3 cells were treated with or without H 2 O 2 (10 µM) for 12 hours. Total RNAs were assayed by semi- quantitative RT-PCR. Mouse β-actin was used for internal control.
Article Snippet:
Techniques: Activity Assay, Luciferase, Transfection, Plasmid Preparation, Quantitative RT-PCR, Control