Journal: iScience

Article Title: Tracking fructose 1,6-bisphosphate dynamics in liver cancer cells using a fluorescent biosensor

doi: 10.1016/j.isci.2024.111336

Figure Lengend Snippet:

Article Snippet: Ni2+-Sepharose High-Performance Agarose Beads , Cytiva , Cat# 17526802.

Techniques: Generated, Produced, Virus, Recombinant, Cell Culture, Lysis, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Plasmid Preparation, Mass Spectrometry, Sequencing, Software, Transfection, Staining, Blocking Assay

Journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Article Title: Purification, crystallization and preliminary X-ray diffraction analysis of the 23S rRNA methyltransferase RlmJ from Escherichia coli

doi: 10.1107/S1744309113020289

Figure Lengend Snippet: ( a ) Elution profile of RlmJ from a HiLoad 16/60 Superdex 75 gel-filtration column. The major peak corresponds to monomeric RlmJ. Elution volumes of protein molecular-mass standards are indicated at the top. ( b ) PhastGel 8–25% gradient SDS–PAGE analysis of RlmJ purification. Lane 1, molecular-weight marker (labelled in kDa); lane 2, cell lysate; lane 3, flowthrough from Ni Sepharose column; lane 4, final wash fraction from Ni Sepharose column; lanes 5–7, elution fractions from Ni Sepharose column; lane 8, final concentrated RlmJ after HiLoad 16/60 Superdex 75 gel-filtration column.

Article Snippet: The lysate was clarified by centrifugation, passed through a 0.2 µm filter and loaded onto a Bio-Rad Econo-Pac gravity-flow column containing 1.0 ml Ni Sepharose High Performance (GE Healthcare, Sweden) pre-equilibrated with buffer A containing 10 m M imidazole.

Techniques: Filtration, SDS Page, Purification, Molecular Weight, Marker