Journal: Environmental Health Perspectives

Article Title: Seasonal Variations in Air Pollution Particle-Induced Inflammatory Mediator Release and Oxidative Stress

doi: 10.1289/ehp.7996

Figure Lengend Snippet: Production of ( A ) IL-6 by AM and ( B ) IL-8 by NHBE cells stimulated with coarse, fine, and ultrafine Chapel Hill pollution particles collected in four different months. The control IL-8 and IL-6 concentrations were 1.7 ± 0.2 ng/mL and 198 ± 5 pg/mL, respectively. Particles were added to NHBE cells at 11 μg/mL and to AMs at 50 μg/mL. *Tukey adjusted p -value < 0.05; n = 3–4 each. The dashed line denotes 1.0 (no change over control). The brackets indicate the groups that are significantly different by ANOVA and the Tukey subtest.

Article Snippet: Typically at confluency, NHBE cells in the 12-well plate (catalog no. 3512; Costar, Corning, NY) had a density of 300,000–500,000 cells/well in 2 mL of BEGM.

Techniques:

Journal: Environmental Health Perspectives

Article Title: Seasonal Variations in Air Pollution Particle-Induced Inflammatory Mediator Release and Oxidative Stress

doi: 10.1289/ehp.7996

Figure Lengend Snippet: Production of ROSs measured by DCF in ( A ) AM and ( B ) NHBE cells stimulated with coarse, fine, and ultrafine Chapel Hill pollution particles collected in four different months. Particles were added to NHBE cells at 11 μg/mL and to AMs at 50 μg/mL. In general, NHBE cells appeared to be more responsive to PM than the AMs because increases in DCF signals were similar between the two cell types even though NHBE cells were exposed to lower doses of PM. *Tukey adjusted p -value < 0.05; # p < 0.05 versus control (ratio = 1.0) by the one-group Student t -test; n = 3–4 each. The dashed line denotes 1.0 (no change over control). The brackets indicate groups that are significantly different by the Tukey subtest.

Article Snippet: Typically at confluency, NHBE cells in the 12-well plate (catalog no. 3512; Costar, Corning, NY) had a density of 300,000–500,000 cells/well in 2 mL of BEGM.

Techniques:

Journal: Environmental Health Perspectives

Article Title: Seasonal Variations in Air Pollution Particle-Induced Inflammatory Mediator Release and Oxidative Stress

doi: 10.1289/ehp.7996

Figure Lengend Snippet: Production of ROSs measured by DHR in ( A ) AM and ( B ) NHBE cells stimulated with coarse, fine, and ultrafine Chapel Hill pollution particles collected in four different months. Particles were added to NHBE cells at 11 μg/mL and to AMs at 50 μg/mL. In general, NHBE cells appeared more responsive to PM than were AMs because increases in DHR signals were similar between the two cell types even though NHBE cells were exposed to lower doses of PM. *Tukey adjusted p -value < 0.05; n = 3–4 each. The dashed line denotes 1.0 (no change over control). The brackets indicate groups that are significantly different by ANOVA and the Tukey subtest.

Article Snippet: Typically at confluency, NHBE cells in the 12-well plate (catalog no. 3512; Costar, Corning, NY) had a density of 300,000–500,000 cells/well in 2 mL of BEGM.

Techniques:

Journal: Environmental Health Perspectives

Article Title: Seasonal Variations in Air Pollution Particle-Induced Inflammatory Mediator Release and Oxidative Stress

doi: 10.1289/ehp.7996

Figure Lengend Snippet: Effects of PM from 12 different months on the release of ( A ) IL-6 in AMs and ( B ) IL-8 in NHBE cells. Particles were added to AMs at 50 μg/mL and to NHBE cells at 11 μg/mL. The data for December ultrafine PM were not available and were omitted in the figure; n = 3–4 each.

Article Snippet: Typically at confluency, NHBE cells in the 12-well plate (catalog no. 3512; Costar, Corning, NY) had a density of 300,000–500,000 cells/well in 2 mL of BEGM.

Techniques:

Journal: Environmental Health Perspectives

Article Title: Seasonal Variations in Air Pollution Particle-Induced Inflammatory Mediator Release and Oxidative Stress

doi: 10.1289/ehp.7996

Figure Lengend Snippet: Correlations between Cr and IL-8 release in NHBE cells incubated with ( A ) coarse, ( B ) fine, and ( C ) ultrafine PM from 12 months. The dashed line represents the linear regression line. The overall model p -values for coarse, fine, and ultrafine PM were 0.106, 0.003, and 0.036, respectively. R 2 = 0.6082 and 0.401 for fine and ultrafine PM, respectively.

Article Snippet: Typically at confluency, NHBE cells in the 12-well plate (catalog no. 3512; Costar, Corning, NY) had a density of 300,000–500,000 cells/well in 2 mL of BEGM.

Techniques: Incubation

Journal: The Journal of Nutrition

Article Title: Candidate Dietary Phytochemicals Modulate Expression of Phase II Enzymes GSTP1 and NQO1 in Human Lung Cells 1–3

doi: 10.3945/jn.110.121905

Figure Lengend Snippet: Cytotoxicity of GTE, BSE, EGCG, SFN, PEITC, and BITC in NHBE cells. (A) Cells were treated with each of the agents for 48 h. Data are means ± SEM, n = 2 means of triplicate measures. For each agent, the effect of the dose was significant. Agents differed significantly from one another at 2 units/L with differences shown at 20 units/L where means without a common letter differ, P < 0.05. (B) Phase contrast images of NHBE cells after 48 h treatments.

Article Snippet: NHBE cells (BioWhittaker) were maintained in bronchial epithelial cell growth medium containing Clonetics bronchial epithelial cell basal medium with supplements provided by BioWhittaker.

Techniques:

Journal: The Journal of Nutrition

Article Title: Candidate Dietary Phytochemicals Modulate Expression of Phase II Enzymes GSTP1 and NQO1 in Human Lung Cells 1–3

doi: 10.3945/jn.110.121905

Figure Lengend Snippet: Modulation of GSTP1 (A–E) and NQO1 (F–J) mRNA expression by the chemopreventive agents in NHBE cells. Values are expressed as fold of vehicle-treated control at that time and are means ± SEM, n = 2 means of triplicate measures. Different from the vehicle-treated control at that time, *P < 0.05.

Article Snippet: NHBE cells (BioWhittaker) were maintained in bronchial epithelial cell growth medium containing Clonetics bronchial epithelial cell basal medium with supplements provided by BioWhittaker.

Techniques: Expressing, Control

Journal: The Journal of Nutrition

Article Title: Candidate Dietary Phytochemicals Modulate Expression of Phase II Enzymes GSTP1 and NQO1 in Human Lung Cells 1–3

doi: 10.3945/jn.110.121905

Figure Lengend Snippet: Modulation of GSTP1 and NQO1 protein expression by the chemopreventive agents in NHBE cells. (A–C) Cells were treated with 0.1% water or 0.1% DMSO vehicle and 2.0 μmol/L EGCG, SFN, PEITC, or BITC, and 2.0 ng/L GTE or BSE for 24 h (A), 48 h (B), and 6 d (C), respectively. D, E: Densitometry analysis of relative GSTP1 (D) and NQO1 (E) protein expression. Values are expressed as fold of the untreated control at that time and are means ± SEM, n = 3. Different from the untreated control at that time, *P < 0.05, ** P < 0.01. O, untreated control.

Article Snippet: NHBE cells (BioWhittaker) were maintained in bronchial epithelial cell growth medium containing Clonetics bronchial epithelial cell basal medium with supplements provided by BioWhittaker.

Techniques: Expressing, Control

Journal: Biomaterials Research

Article Title: Advanced pathophysiology mimicking lung models for accelerated drug discovery

doi: 10.1186/s40824-023-00366-x

Figure Lengend Snippet: The impedance measurement of CNBio NHBE-NHLF models was performed every 2–3 days during 24 days. The left panels indicated the TEER value and the right panels indicated cell capacitance. The TEER value (from Day 3 to Day 15) and cell capacitance (from Day 13 to Day 24) were presented for ( A ) CNBio NHBE-NHLF coated with collagen on the Corning insert ( B ) CNBio NHBE-NHLF coated with collagen on the CellQart insert ( C ) CNBio NHBE-NHLF coated with fibronectin on the Corning insert ( D ) CNBio NHBE-NHLF coated with collagen on the Corning insert. n = 3. E 3D topographical AFM images and linear regression analysis of the stiffness of the Corning and CellQart inserts. The stiffness analyses of the Corning and CellQart inserts were generated from the force-volume map of the Corning insert (800 points) and 40 individual force-distance curves of the CellQart insert

Article Snippet: The TEER value (from Day 3 to Day 15) and cell capacitance (from Day 13 to Day 24) were presented for ( A ) CNBio NHBE-NHLF coated with collagen on the Corning insert ( B ) CNBio NHBE-NHLF coated with collagen on the CellQart insert ( C ) CNBio NHBE-NHLF coated with fibronectin on the Corning insert ( D ) CNBio NHBE-NHLF coated with collagen on the Corning insert. n = 3.

Techniques: Generated