nh4 hco3 Search Results


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  • 95
    Millipore nh4
    Nh4, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nh4 - by Bioz Stars, 2020-08
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    nh4  (Difco)
    92
    Difco nh4
    Nh4, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nh4 - by Bioz Stars, 2020-08
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    nh4  (Nacalai)
    92
    Nacalai nh4
    Nh4, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cambridge Isotope Laboratories nh4
    Nh4, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nh4 - by Bioz Stars, 2020-08
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    92
    Fisher Scientific nh4 oh
    Nh4 Oh, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nh4 oh - by Bioz Stars, 2020-08
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    92
    AnaSpec nh4 oh
    Nh4 Oh, supplied by AnaSpec, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nh4 oh - by Bioz Stars, 2020-08
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    93
    Millipore nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 4666 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore nh4 hco3
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Hco3, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2035 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore nh4 oh
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Oh, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck & Co nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nh4 cl - by Bioz Stars, 2020-08
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    92
    Millipore nh4 f
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 F, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Formedium nh4 no3
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 No3, supplied by Formedium, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cambridge Isotope Laboratories nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 4688 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore nh4 no3
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 No3, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sangon Biotech nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nh4 cl - by Bioz Stars, 2020-08
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    92
    Applichem nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by Applichem, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nh4 cl - by Bioz Stars, 2020-08
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    91
    Thermo Fisher nh4 hf
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Hf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore nh4 scn
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Scn, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    BioShop nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by BioShop, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor nh4 oh
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Oh, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher nh4 oh
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Oh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA nh4 cl
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
    Nh4 Cl, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nh4 cl - by Bioz Stars, 2020-08
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    92
    FUJIFILM nh4 hco3
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
    Nh4 Hco3, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nh4 hco3 - by Bioz Stars, 2020-08
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    91
    Merck & Co nh4 no3
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
    Nh4 No3, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nh4 no3 - by Bioz Stars, 2020-08
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    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl (NH4). Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p

    Journal: Acta Neuropathologica

    Article Title: Intraneuronal aggregation of the β-CTF fragment of APP (C99) induces Aβ-independent lysosomal-autophagic pathology

    doi: 10.1007/s00401-016-1577-6

    Figure Lengend Snippet: γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl (NH4). Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p

    Article Snippet: Cell culture and experimental treatments The human neuroblastoma cell line SH-SY5Y, naive or stably expressing APPswe or pcDNA3 (mock), previously described [ ] was exposed to the following drugs for 16–20 h: D6 (50 nM to 5 μM, Elan Pharmaceuticals, San Francisco) in vehicle (methylcellulose/polysorbate 80, Sigma), DAPT (5 μM in DMSO, Sigma), leupeptin (10 μM in H2 O, Sigma), PADK (5 μM in DMSO, Bachem), NH4 Cl (10 mM in H2 O, Sigma), pepstatin A (10 μM in EtOH, Enzo Life sciences), smer28 (50 μM in DMSO, Sigma) and bafilomycin A1 (50 nM in H20, Sigma).

    Techniques: Expressing, Transfection, Western Blot, Staining, Activity Assay, In Vitro

    Phylogenetic tree of OTU 0.03 s related to taxa of NH4 + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).

    Journal: Applied and Environmental Microbiology

    Article Title: Internal Porosity of Mineral Coating Supports Microbial Activity in Rapid Sand Filters for Groundwater Treatment

    doi: 10.1128/AEM.01959-14

    Figure Lengend Snippet: Phylogenetic tree of OTU 0.03 s related to taxa of NH4 + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).

    Article Snippet: Effluent water from the investigated full-scale rapid sand filter was used for the column influents, supplemented with 1 mg/liter NH4 + -N as NH4 Cl (Merck Chemicals).

    Techniques: Construct, Sequencing