ngal Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Thermo Fisher anti ngal mab
    Downregulation of <t>E-cadherin</t> reduces <t>NGAL</t> expression
    Anti Ngal Mab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ngal mab/product/Thermo Fisher
    Average 86 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    anti ngal mab - by Bioz Stars, 2020-08
    86/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology ngal
    Paired analysis of <t>MMP-9</t> activities vs. protein levels in T1D urine samples. A : gelatin substrate zymography of T1D urine samples ( lanes 1–7 ) show zones that represent MMP-2 (72 kDa), MMP-9 (92 kDa), and <t>NGAL/MMP-9</t> complexes (125 kDa) relative
    Ngal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngal/product/Santa Cruz Biotechnology
    Average 92 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    ngal - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    89
    BioPorto urinary ngal
    Renal biomarkers in plasma and urine. Time course box blot analysis for plasma (left columns) and urinary (right column) biomarkers including creatinine, eGFR, albumin, <t>NGAL,</t> L-FABP, cystatin C and KIM1. Bright columns (left at each time point) represent <t>non-AKI</t> patients, dark columns (right at each time point) AKI patients. Biomarkers in urine are normalized to urinary creatinine (uCrea). Boxplots display median, 25th and 75th percentiles. The unadjusted p-values of Wilcoxon rank-sum test are depicted above the boxplots.
    Urinary Ngal, supplied by BioPorto, used in various techniques. Bioz Stars score: 89/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/urinary ngal/product/BioPorto
    Average 89 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    urinary ngal - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    85
    Abcam lcn2 ngal
    13- cis RA and ATRA increase <t>LCN2</t> mRNA and <t>NGAL</t> protein expression in SEB-1 sebocytes. SEB-1 sebocytes were treated with vehicle as a control (C), 13- cis RA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) or ATRA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) for 48 and 72 hours. ( A ) LCN2 mRNA expression was verified by QPCR after 48 hours of retinoid treatment. Data represent mean ± SEM of the fold change in gene expression as determined by QPCR of 5 independent samples. Statistical analysis was performed with REST-XL software program and considered significant if * P
    Lcn2 Ngal, supplied by Abcam, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lcn2 ngal/product/Abcam
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lcn2 ngal - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    93
    Getein Biotech ngal kit
    13- cis RA and ATRA increase <t>LCN2</t> mRNA and <t>NGAL</t> protein expression in SEB-1 sebocytes. SEB-1 sebocytes were treated with vehicle as a control (C), 13- cis RA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) or ATRA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) for 48 and 72 hours. ( A ) LCN2 mRNA expression was verified by QPCR after 48 hours of retinoid treatment. Data represent mean ± SEM of the fold change in gene expression as determined by QPCR of 5 independent samples. Statistical analysis was performed with REST-XL software program and considered significant if * P
    Ngal Kit, supplied by Getein Biotech, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngal kit/product/Getein Biotech
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ngal kit - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    92
    Thermo Fisher ngal
    13- cis RA and ATRA increase <t>LCN2</t> mRNA and <t>NGAL</t> protein expression in SEB-1 sebocytes. SEB-1 sebocytes were treated with vehicle as a control (C), 13- cis RA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) or ATRA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) for 48 and 72 hours. ( A ) LCN2 mRNA expression was verified by QPCR after 48 hours of retinoid treatment. Data represent mean ± SEM of the fold change in gene expression as determined by QPCR of 5 independent samples. Statistical analysis was performed with REST-XL software program and considered significant if * P
    Ngal, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngal/product/Thermo Fisher
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    ngal - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Vigene Biosciences ad ngal
    13- cis RA and ATRA increase <t>LCN2</t> mRNA and <t>NGAL</t> protein expression in SEB-1 sebocytes. SEB-1 sebocytes were treated with vehicle as a control (C), 13- cis RA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) or ATRA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) for 48 and 72 hours. ( A ) LCN2 mRNA expression was verified by QPCR after 48 hours of retinoid treatment. Data represent mean ± SEM of the fold change in gene expression as determined by QPCR of 5 independent samples. Statistical analysis was performed with REST-XL software program and considered significant if * P
    Ad Ngal, supplied by Vigene Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ad ngal/product/Vigene Biosciences
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ad ngal - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    89
    BioPorto urine ngal
    13- cis RA and ATRA increase <t>LCN2</t> mRNA and <t>NGAL</t> protein expression in SEB-1 sebocytes. SEB-1 sebocytes were treated with vehicle as a control (C), 13- cis RA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) or ATRA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) for 48 and 72 hours. ( A ) LCN2 mRNA expression was verified by QPCR after 48 hours of retinoid treatment. Data represent mean ± SEM of the fold change in gene expression as determined by QPCR of 5 independent samples. Statistical analysis was performed with REST-XL software program and considered significant if * P
    Urine Ngal, supplied by BioPorto, used in various techniques. Bioz Stars score: 89/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/urine ngal/product/BioPorto
    Average 89 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    urine ngal - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    ngal  (Abcam)
    94
    Abcam ngal
    The effect of diabetes on <t>NGAL</t> and <t>MMP-9</t> expression in PVC implant cells and wound fluids at days 3, 6 and 12. (A) NGAL, MMP-9 and MMP-8 mRNA by qRT-PCR, (B) A representative zymogram of wound fluid at day 6, (C-E) Group data for zymography results expressed as Area Under Curve (AUC; determined from band intensity) (C) total MMP-9 (TMMP-9 = proMMP-9 +active MMP-9), (D) active MMP-9, (E) proMMP-9 and (F) NGAL/MMP-9 complex according to molecular weight. Results are from Control (CON) diabetic (DM) and Insulin treated DM (DM+INS) animals and are expressed as Mean ± SEM *P
    Ngal, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngal/product/Abcam
    Average 94 stars, based on 117 article reviews
    Price from $9.99 to $1999.99
    ngal - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    92
    R&D Systems ngal duosets
    The effect of diabetes on <t>NGAL</t> and <t>MMP-9</t> expression in PVC implant cells and wound fluids at days 3, 6 and 12. (A) NGAL, MMP-9 and MMP-8 mRNA by qRT-PCR, (B) A representative zymogram of wound fluid at day 6, (C-E) Group data for zymography results expressed as Area Under Curve (AUC; determined from band intensity) (C) total MMP-9 (TMMP-9 = proMMP-9 +active MMP-9), (D) active MMP-9, (E) proMMP-9 and (F) NGAL/MMP-9 complex according to molecular weight. Results are from Control (CON) diabetic (DM) and Insulin treated DM (DM+INS) animals and are expressed as Mean ± SEM *P
    Ngal Duosets, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngal duosets/product/R&D Systems
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ngal duosets - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    R&D Systems ngal plates
    The effect of diabetes on <t>NGAL</t> and <t>MMP-9</t> expression in PVC implant cells and wound fluids at days 3, 6 and 12. (A) NGAL, MMP-9 and MMP-8 mRNA by qRT-PCR, (B) A representative zymogram of wound fluid at day 6, (C-E) Group data for zymography results expressed as Area Under Curve (AUC; determined from band intensity) (C) total MMP-9 (TMMP-9 = proMMP-9 +active MMP-9), (D) active MMP-9, (E) proMMP-9 and (F) NGAL/MMP-9 complex according to molecular weight. Results are from Control (CON) diabetic (DM) and Insulin treated DM (DM+INS) animals and are expressed as Mean ± SEM *P
    Ngal Plates, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngal plates/product/R&D Systems
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ngal plates - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    91
    R&D Systems unbound ngal
    The effect of diabetes on <t>NGAL</t> and <t>MMP-9</t> expression in PVC implant cells and wound fluids at days 3, 6 and 12. (A) NGAL, MMP-9 and MMP-8 mRNA by qRT-PCR, (B) A representative zymogram of wound fluid at day 6, (C-E) Group data for zymography results expressed as Area Under Curve (AUC; determined from band intensity) (C) total MMP-9 (TMMP-9 = proMMP-9 +active MMP-9), (D) active MMP-9, (E) proMMP-9 and (F) NGAL/MMP-9 complex according to molecular weight. Results are from Control (CON) diabetic (DM) and Insulin treated DM (DM+INS) animals and are expressed as Mean ± SEM *P
    Unbound Ngal, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unbound ngal/product/R&D Systems
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    unbound ngal - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    89
    R&D Systems urinary ngal
    Box-plot graphs for recipients’ serum Klotho ( A ), <t>FGF-23</t> ( B ), and urinary <t>NGAL</t> ( C ) at D0, M1, M3, and Y1 post-transplant. FGF-23 – fibroblast growth factor-23; NGAL – neutrophil gelatinase-associated lipocalin; * p
    Urinary Ngal, supplied by R&D Systems, used in various techniques. Bioz Stars score: 89/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/urinary ngal/product/R&D Systems
    Average 89 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    urinary ngal - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    89
    USCNK serum ngal
    Urinary <t>alpha-GST</t> (a), pi-GST (b), <t>NGAL</t> (c, d), and KIM-1 (e) ratios in patients with obstructive nephropathy (ON), patients with hydronephrosis without obstructive nephropathy (HN without ON), and controls.
    Serum Ngal, supplied by USCNK, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/serum ngal/product/USCNK
    Average 89 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    serum ngal - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    86
    R&D Systems mouse ngal
    AKI-marker <t>NGAL</t> serum levels and tissue gene expression of P-Selectin and ICAM-1 as well as NGAL were significantly reduced after IR in mice treated with Bβ 15–42 compared to untreated mice. ( A ) Tissue gene expression of adhesion molecules P-Selectin (left graph) and ICAM-1 (right graph). ( B ) Tissue gene expression of the AKI marker NGAL (left graph). Circulating protein levels of serum NGAL (right graph). Values are expressed in % of NaCl for mRNA analyses and in ng/ml for <t>ELISA</t> measurements. All experiments were performed in duplicates. Error bars represent means ± SEM. *P
    Mouse Ngal, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse ngal/product/R&D Systems
    Average 86 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    mouse ngal - by Bioz Stars, 2020-08
    86/100 stars
      Buy from Supplier

    90
    R&D Systems plasma ngal
    FGF23 levels rises early after renal injury. Time course data were obtained from time 0, 1, 2, 4, 6, and 8 hours after FA or vehicle injection. N=7-9 for time-points 0-4, and N=3-5 for time points 6 and 8; shown are mean values ±SEM from two independent experiments. *Indicates the earliest time point at which a significant difference between FA (solid line) and vehicle (dotted line) injected animals was observed. A. Plasma BUN levels (mg/dL). B. Plasma phosphate levels (mg/dL). C. Plasma <t>iFGF23</t> levels (pg/mL). D. Plasma <t>NGAL</t> levels (ng/mL). E. Plasma cFGF23 levels (pg/mL).
    Plasma Ngal, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasma ngal/product/R&D Systems
    Average 90 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    plasma ngal - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    88
    R&D Systems recombinant ngal
    In vitro analysis of <t>NGAL</t> expression and release by human tubular epithelial cells <t>(TEC)</t> in different experimental conditions. Relative quantification analysis (A) of NGAL mRNA expression in TEC evaluated by qRT-PCR; mRNA expression of 3 different experiments was normalized for both GAPDH (black columns) and Beta-Actin (white columns) after calculating housekeeping gene variation (see also methods ). Hypoxia and 20 ng/ml Tacrolimus (Tac) significantly increased NGAL mRNA expression when compared to basal culture condition (Control, *p
    Recombinant Ngal, supplied by R&D Systems, used in various techniques. Bioz Stars score: 88/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant ngal/product/R&D Systems
    Average 88 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    recombinant ngal - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    90
    BioPorto ngal assay kit
    In vitro analysis of <t>NGAL</t> expression and release by human tubular epithelial cells <t>(TEC)</t> in different experimental conditions. Relative quantification analysis (A) of NGAL mRNA expression in TEC evaluated by qRT-PCR; mRNA expression of 3 different experiments was normalized for both GAPDH (black columns) and Beta-Actin (white columns) after calculating housekeeping gene variation (see also methods ). Hypoxia and 20 ng/ml Tacrolimus (Tac) significantly increased NGAL mRNA expression when compared to basal culture condition (Control, *p
    Ngal Assay Kit, supplied by BioPorto, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngal assay kit/product/BioPorto
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    ngal assay kit - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    93
    Abcam canine ngal elisa kit
    In vitro analysis of <t>NGAL</t> expression and release by human tubular epithelial cells <t>(TEC)</t> in different experimental conditions. Relative quantification analysis (A) of NGAL mRNA expression in TEC evaluated by qRT-PCR; mRNA expression of 3 different experiments was normalized for both GAPDH (black columns) and Beta-Actin (white columns) after calculating housekeeping gene variation (see also methods ). Hypoxia and 20 ng/ml Tacrolimus (Tac) significantly increased NGAL mRNA expression when compared to basal culture condition (Control, *p
    Canine Ngal Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/canine ngal elisa kit/product/Abcam
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    canine ngal elisa kit - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    Thermo Fisher ngal mouse elisa kit
    In vitro analysis of <t>NGAL</t> expression and release by human tubular epithelial cells <t>(TEC)</t> in different experimental conditions. Relative quantification analysis (A) of NGAL mRNA expression in TEC evaluated by qRT-PCR; mRNA expression of 3 different experiments was normalized for both GAPDH (black columns) and Beta-Actin (white columns) after calculating housekeeping gene variation (see also methods ). Hypoxia and 20 ng/ml Tacrolimus (Tac) significantly increased NGAL mRNA expression when compared to basal culture condition (Control, *p
    Ngal Mouse Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngal mouse elisa kit/product/Thermo Fisher
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    ngal mouse elisa kit - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    Enzo Biochem rat ngal
    In vitro analysis of <t>NGAL</t> expression and release by human tubular epithelial cells <t>(TEC)</t> in different experimental conditions. Relative quantification analysis (A) of NGAL mRNA expression in TEC evaluated by qRT-PCR; mRNA expression of 3 different experiments was normalized for both GAPDH (black columns) and Beta-Actin (white columns) after calculating housekeeping gene variation (see also methods ). Hypoxia and 20 ng/ml Tacrolimus (Tac) significantly increased NGAL mRNA expression when compared to basal culture condition (Control, *p
    Rat Ngal, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat ngal/product/Enzo Biochem
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rat ngal - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    BioPorto ngal calibrator 5
    In vitro analysis of <t>NGAL</t> expression and release by human tubular epithelial cells <t>(TEC)</t> in different experimental conditions. Relative quantification analysis (A) of NGAL mRNA expression in TEC evaluated by qRT-PCR; mRNA expression of 3 different experiments was normalized for both GAPDH (black columns) and Beta-Actin (white columns) after calculating housekeeping gene variation (see also methods ). Hypoxia and 20 ng/ml Tacrolimus (Tac) significantly increased NGAL mRNA expression when compared to basal culture condition (Control, *p
    Ngal Calibrator 5, supplied by BioPorto, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngal calibrator 5/product/BioPorto
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ngal calibrator 5 - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    Downregulation of E-cadherin reduces NGAL expression

    Journal: Cancer

    Article Title: Epidermal growth factor down-regulates the expression of neutrophil gelatinase-associated lipocalin (NGAL) through E-cadherin in pancreatic cancer cells

    doi: 10.1002/cncr.25803

    Figure Lengend Snippet: Downregulation of E-cadherin reduces NGAL expression

    Article Snippet: EGF was purchased from Sigma (St. Louis, MO), PD98059, U0126, E-cadherin and phospho-ERK antibodies from Cell Signaling Technology (Danvers, MA), AG1478 from Calbiochem (Gibbstown, NJ) and mouse monoclonal NGAL antibodies from Antibody Shop (Gentofte, Demark). pLKO-shE-cadherin and its control vectors were gifts from Dr. Sendurai Mani (MD Anderson Cancer Center, Houston, TX). pCMV-SPORT6-E-cadherin was purchased from OPEN Biosystems (Huntsville, AL).

    Techniques: Expressing

    E-cadherin overexpression leads to increased NGAL expression

    Journal: Cancer

    Article Title: Epidermal growth factor down-regulates the expression of neutrophil gelatinase-associated lipocalin (NGAL) through E-cadherin in pancreatic cancer cells

    doi: 10.1002/cncr.25803

    Figure Lengend Snippet: E-cadherin overexpression leads to increased NGAL expression

    Article Snippet: EGF was purchased from Sigma (St. Louis, MO), PD98059, U0126, E-cadherin and phospho-ERK antibodies from Cell Signaling Technology (Danvers, MA), AG1478 from Calbiochem (Gibbstown, NJ) and mouse monoclonal NGAL antibodies from Antibody Shop (Gentofte, Demark). pLKO-shE-cadherin and its control vectors were gifts from Dr. Sendurai Mani (MD Anderson Cancer Center, Houston, TX). pCMV-SPORT6-E-cadherin was purchased from OPEN Biosystems (Huntsville, AL).

    Techniques: Over Expression, Expressing

    Paired analysis of MMP-9 activities vs. protein levels in T1D urine samples. A : gelatin substrate zymography of T1D urine samples ( lanes 1–7 ) show zones that represent MMP-2 (72 kDa), MMP-9 (92 kDa), and NGAL/MMP-9 complexes (125 kDa) relative

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Urinary matrix metalloproteinase activities: biomarkers for plaque angiogenesis and nephropathy in diabetes

    doi: 10.1152/ajprenal.00267.2011

    Figure Lengend Snippet: Paired analysis of MMP-9 activities vs. protein levels in T1D urine samples. A : gelatin substrate zymography of T1D urine samples ( lanes 1–7 ) show zones that represent MMP-2 (72 kDa), MMP-9 (92 kDa), and NGAL/MMP-9 complexes (125 kDa) relative

    Article Snippet: MMP were detected in kidney and aorta tissues using primary antibodies reactive to NGAL (0.4 μg/ml, sc-50351, Santa Cruz Biotechnology), MMP-2 (1 μg/ml, AB19167, Millipore), and MMP-9 (1 μg/ml, ab38898, Abcam).

    Techniques: Zymography

    Urinary MMP activities in T1D adolescents. Urinary MMP-2 ( A ), MMP-9 ( B ), and NGAL/MMP-9 ( C ) were compared between T1D and ND adolescents. Relative activity units for MMP-2 and NGAL/MMP-9 were increased in T1D ( P = 0.002 and P = 0.048, respectively; Kruskal-Wallis

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Urinary matrix metalloproteinase activities: biomarkers for plaque angiogenesis and nephropathy in diabetes

    doi: 10.1152/ajprenal.00267.2011

    Figure Lengend Snippet: Urinary MMP activities in T1D adolescents. Urinary MMP-2 ( A ), MMP-9 ( B ), and NGAL/MMP-9 ( C ) were compared between T1D and ND adolescents. Relative activity units for MMP-2 and NGAL/MMP-9 were increased in T1D ( P = 0.002 and P = 0.048, respectively; Kruskal-Wallis

    Article Snippet: MMP were detected in kidney and aorta tissues using primary antibodies reactive to NGAL (0.4 μg/ml, sc-50351, Santa Cruz Biotechnology), MMP-2 (1 μg/ml, AB19167, Millipore), and MMP-9 (1 μg/ml, ab38898, Abcam).

    Techniques: Activity Assay

    Urinary MMP activities in rats with T1D nephropathy. A : urinary MMP-2 ( top ), MMP-9 ( middle ), and NGAL/MMP-9 ( bottom ) were compared between ND (○) and T1D rats (●) after 13 wk of T1D. Relative activities (see materials and methods ) were

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Urinary matrix metalloproteinase activities: biomarkers for plaque angiogenesis and nephropathy in diabetes

    doi: 10.1152/ajprenal.00267.2011

    Figure Lengend Snippet: Urinary MMP activities in rats with T1D nephropathy. A : urinary MMP-2 ( top ), MMP-9 ( middle ), and NGAL/MMP-9 ( bottom ) were compared between ND (○) and T1D rats (●) after 13 wk of T1D. Relative activities (see materials and methods ) were

    Article Snippet: MMP were detected in kidney and aorta tissues using primary antibodies reactive to NGAL (0.4 μg/ml, sc-50351, Santa Cruz Biotechnology), MMP-2 (1 μg/ml, AB19167, Millipore), and MMP-9 (1 μg/ml, ab38898, Abcam).

    Techniques:

    MMP localization in T1D kidney. MMP-9 ( A and B ), NGAL ( C and D ), and MMP-2 ( E and F ) are shown in kidneys from ND ( A , C , and E ) and T1D ( B , D , and F ) rats. MMP-9 localizes on blood vessels, tubules, and MMP-9-positive mononuclear cells that infiltrate

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Urinary matrix metalloproteinase activities: biomarkers for plaque angiogenesis and nephropathy in diabetes

    doi: 10.1152/ajprenal.00267.2011

    Figure Lengend Snippet: MMP localization in T1D kidney. MMP-9 ( A and B ), NGAL ( C and D ), and MMP-2 ( E and F ) are shown in kidneys from ND ( A , C , and E ) and T1D ( B , D , and F ) rats. MMP-9 localizes on blood vessels, tubules, and MMP-9-positive mononuclear cells that infiltrate

    Article Snippet: MMP were detected in kidney and aorta tissues using primary antibodies reactive to NGAL (0.4 μg/ml, sc-50351, Santa Cruz Biotechnology), MMP-2 (1 μg/ml, AB19167, Millipore), and MMP-9 (1 μg/ml, ab38898, Abcam).

    Techniques:

    Renal biomarkers in plasma and urine. Time course box blot analysis for plasma (left columns) and urinary (right column) biomarkers including creatinine, eGFR, albumin, NGAL, L-FABP, cystatin C and KIM1. Bright columns (left at each time point) represent non-AKI patients, dark columns (right at each time point) AKI patients. Biomarkers in urine are normalized to urinary creatinine (uCrea). Boxplots display median, 25th and 75th percentiles. The unadjusted p-values of Wilcoxon rank-sum test are depicted above the boxplots.

    Journal: PLoS ONE

    Article Title: Comparison of Plasma and Urine Biomarker Performance in Acute Kidney Injury

    doi: 10.1371/journal.pone.0145042

    Figure Lengend Snippet: Renal biomarkers in plasma and urine. Time course box blot analysis for plasma (left columns) and urinary (right column) biomarkers including creatinine, eGFR, albumin, NGAL, L-FABP, cystatin C and KIM1. Bright columns (left at each time point) represent non-AKI patients, dark columns (right at each time point) AKI patients. Biomarkers in urine are normalized to urinary creatinine (uCrea). Boxplots display median, 25th and 75th percentiles. The unadjusted p-values of Wilcoxon rank-sum test are depicted above the boxplots.

    Article Snippet: ROC curves for plasma and urinary NGAL to detect postoperative AKI 4 hours after CPB.

    Techniques:

    13- cis RA and ATRA increase LCN2 mRNA and NGAL protein expression in SEB-1 sebocytes. SEB-1 sebocytes were treated with vehicle as a control (C), 13- cis RA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) or ATRA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) for 48 and 72 hours. ( A ) LCN2 mRNA expression was verified by QPCR after 48 hours of retinoid treatment. Data represent mean ± SEM of the fold change in gene expression as determined by QPCR of 5 independent samples. Statistical analysis was performed with REST-XL software program and considered significant if * P

    Journal: The Journal of Clinical Investigation

    Article Title: Neutrophil gelatinase-associated lipocalin mediates 13-cis retinoic acid-induced apoptosis of human sebaceous gland cells

    doi: 10.1172/JCI33869

    Figure Lengend Snippet: 13- cis RA and ATRA increase LCN2 mRNA and NGAL protein expression in SEB-1 sebocytes. SEB-1 sebocytes were treated with vehicle as a control (C), 13- cis RA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) or ATRA (0.1 nM, 1 nM, 10 nM, 0.1 μM, or 1 μM) for 48 and 72 hours. ( A ) LCN2 mRNA expression was verified by QPCR after 48 hours of retinoid treatment. Data represent mean ± SEM of the fold change in gene expression as determined by QPCR of 5 independent samples. Statistical analysis was performed with REST-XL software program and considered significant if * P

    Article Snippet: Mouse monoclonal antibody to LCN2 (NGAL) was obtained from Abcam Inc.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Software, IF-P

    The effect of diabetes on NGAL and MMP-9 expression in PVC implant cells and wound fluids at days 3, 6 and 12. (A) NGAL, MMP-9 and MMP-8 mRNA by qRT-PCR, (B) A representative zymogram of wound fluid at day 6, (C-E) Group data for zymography results expressed as Area Under Curve (AUC; determined from band intensity) (C) total MMP-9 (TMMP-9 = proMMP-9 +active MMP-9), (D) active MMP-9, (E) proMMP-9 and (F) NGAL/MMP-9 complex according to molecular weight. Results are from Control (CON) diabetic (DM) and Insulin treated DM (DM+INS) animals and are expressed as Mean ± SEM *P

    Journal: PLoS ONE

    Article Title: Insulin treatment prevents wounding associated changes in tissue and circulating neutrophil MMP-9 and NGAL in diabetic rats

    doi: 10.1371/journal.pone.0170951

    Figure Lengend Snippet: The effect of diabetes on NGAL and MMP-9 expression in PVC implant cells and wound fluids at days 3, 6 and 12. (A) NGAL, MMP-9 and MMP-8 mRNA by qRT-PCR, (B) A representative zymogram of wound fluid at day 6, (C-E) Group data for zymography results expressed as Area Under Curve (AUC; determined from band intensity) (C) total MMP-9 (TMMP-9 = proMMP-9 +active MMP-9), (D) active MMP-9, (E) proMMP-9 and (F) NGAL/MMP-9 complex according to molecular weight. Results are from Control (CON) diabetic (DM) and Insulin treated DM (DM+INS) animals and are expressed as Mean ± SEM *P

    Article Snippet: The cellular localisation of NGAL (ab41105; Abcam) and MMP-9 (ab76003; Abcam) was examined in skin wound tissue by immunohistochemistry.

    Techniques: Expressing, Quantitative RT-PCR, Zymography, Molecular Weight

    Peripheral blood neutrophil NGAL and MMP-9 at days 3, 6 and 12 post-implant surgery. (A) Neutrophil number as measured by flow cytometry and (B) Gene expression data for NGAL, MMP-9 and MMP-8. (C) Representative and group data for neutrophil NGAL and MMP-9 and their co-localisation at day 6. Results from Control (CON) diabetic (DM) and Insulin treated DM (DM+INS) animals are expressed as Mean ± SEM. * P

    Journal: PLoS ONE

    Article Title: Insulin treatment prevents wounding associated changes in tissue and circulating neutrophil MMP-9 and NGAL in diabetic rats

    doi: 10.1371/journal.pone.0170951

    Figure Lengend Snippet: Peripheral blood neutrophil NGAL and MMP-9 at days 3, 6 and 12 post-implant surgery. (A) Neutrophil number as measured by flow cytometry and (B) Gene expression data for NGAL, MMP-9 and MMP-8. (C) Representative and group data for neutrophil NGAL and MMP-9 and their co-localisation at day 6. Results from Control (CON) diabetic (DM) and Insulin treated DM (DM+INS) animals are expressed as Mean ± SEM. * P

    Article Snippet: The cellular localisation of NGAL (ab41105; Abcam) and MMP-9 (ab76003; Abcam) was examined in skin wound tissue by immunohistochemistry.

    Techniques: Flow Cytometry, Cytometry, Expressing

    The effect of diabetes on NGAL and MMP-9 in skin wound tissue at day 6. (A) Representative images for skin wound tissue NGAL and MMP-9 respectively (B) Group data for wound NGAL, and MMP-9 mRNA and (C) Group data for percentage MMP-9 staining cells in the granulation tissue. Results are from Control (CON) diabetic (DM) and Insulin treated DM (DM+INS) animals and are expressed as Mean ± SEM. * P

    Journal: PLoS ONE

    Article Title: Insulin treatment prevents wounding associated changes in tissue and circulating neutrophil MMP-9 and NGAL in diabetic rats

    doi: 10.1371/journal.pone.0170951

    Figure Lengend Snippet: The effect of diabetes on NGAL and MMP-9 in skin wound tissue at day 6. (A) Representative images for skin wound tissue NGAL and MMP-9 respectively (B) Group data for wound NGAL, and MMP-9 mRNA and (C) Group data for percentage MMP-9 staining cells in the granulation tissue. Results are from Control (CON) diabetic (DM) and Insulin treated DM (DM+INS) animals and are expressed as Mean ± SEM. * P

    Article Snippet: The cellular localisation of NGAL (ab41105; Abcam) and MMP-9 (ab76003; Abcam) was examined in skin wound tissue by immunohistochemistry.

    Techniques: Staining

    The effect of incubation in high glucose concentration (25mM) on neutrophil (A) NGAL, MMP-9 and MMP-8 mRNA and (B) TLR4, TLR2 and TNFα mRNA. Results are expressed as Mean ± SEM. * P

    Journal: PLoS ONE

    Article Title: Insulin treatment prevents wounding associated changes in tissue and circulating neutrophil MMP-9 and NGAL in diabetic rats

    doi: 10.1371/journal.pone.0170951

    Figure Lengend Snippet: The effect of incubation in high glucose concentration (25mM) on neutrophil (A) NGAL, MMP-9 and MMP-8 mRNA and (B) TLR4, TLR2 and TNFα mRNA. Results are expressed as Mean ± SEM. * P

    Article Snippet: The cellular localisation of NGAL (ab41105; Abcam) and MMP-9 (ab76003; Abcam) was examined in skin wound tissue by immunohistochemistry.

    Techniques: Incubation, Concentration Assay

    The effect of diabetes on PVC implant cell expression of MPO, NGAL and MMP-9 and the co-localisation of MPO with NGAL or MMP-9. (A) Representative day 6 images of implant cells stained with MPO and NGAL either alone or in combination. Group data for percentage of implant cells expressing (B) MPO, NGAL, or MMP-9 or (C) MPO in combination with either NGAL or MMP-9. Data are from the cellular fraction of implants obtained from Control (CON) Diabetic (DM) and Insulin treated DM (DM+INS) animals at days 3, 6 and 12 post implantation. Results are expressed as Mean ± SEM. * P

    Journal: PLoS ONE

    Article Title: Insulin treatment prevents wounding associated changes in tissue and circulating neutrophil MMP-9 and NGAL in diabetic rats

    doi: 10.1371/journal.pone.0170951

    Figure Lengend Snippet: The effect of diabetes on PVC implant cell expression of MPO, NGAL and MMP-9 and the co-localisation of MPO with NGAL or MMP-9. (A) Representative day 6 images of implant cells stained with MPO and NGAL either alone or in combination. Group data for percentage of implant cells expressing (B) MPO, NGAL, or MMP-9 or (C) MPO in combination with either NGAL or MMP-9. Data are from the cellular fraction of implants obtained from Control (CON) Diabetic (DM) and Insulin treated DM (DM+INS) animals at days 3, 6 and 12 post implantation. Results are expressed as Mean ± SEM. * P

    Article Snippet: The cellular localisation of NGAL (ab41105; Abcam) and MMP-9 (ab76003; Abcam) was examined in skin wound tissue by immunohistochemistry.

    Techniques: Expressing, Staining

    Box-plot graphs for recipients’ serum Klotho ( A ), FGF-23 ( B ), and urinary NGAL ( C ) at D0, M1, M3, and Y1 post-transplant. FGF-23 – fibroblast growth factor-23; NGAL – neutrophil gelatinase-associated lipocalin; * p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: The Value of Older Donors’ Klotho Level in Predicting Recipients’ Short-Term Renal Function

    doi: 10.12659/MSM.913274

    Figure Lengend Snippet: Box-plot graphs for recipients’ serum Klotho ( A ), FGF-23 ( B ), and urinary NGAL ( C ) at D0, M1, M3, and Y1 post-transplant. FGF-23 – fibroblast growth factor-23; NGAL – neutrophil gelatinase-associated lipocalin; * p

    Article Snippet: Serum Klotho (Immuno Biological Laboratories Co., Tokyo, Japan, 27998), intact FGF-23 (R & D Systems, Minneapolis, MN, USA, DY2604-05), and urinary NGAL (R & D Systems, Minneapolis, MN, USA, DLCN20) were measured with enzyme-linked immunoadsorbent assay (ELISA) kits.

    Techniques:

    Urinary alpha-GST (a), pi-GST (b), NGAL (c, d), and KIM-1 (e) ratios in patients with obstructive nephropathy (ON), patients with hydronephrosis without obstructive nephropathy (HN without ON), and controls.

    Journal: Disease Markers

    Article Title: Potential Novel Biomarkers of Obstructive Nephropathy in Children with Hydronephrosis

    doi: 10.1155/2018/1015726

    Figure Lengend Snippet: Urinary alpha-GST (a), pi-GST (b), NGAL (c, d), and KIM-1 (e) ratios in patients with obstructive nephropathy (ON), patients with hydronephrosis without obstructive nephropathy (HN without ON), and controls.

    Article Snippet: Urinary alpha-GST (USCNK, China), urinary pi-GST (Immundiagnostik AG, Germany), urinary KIM-1, and urinary and serum NGAL (USCNK, China) concentrations were measured using specific enzyme-linked immunosorbent assay (ELISA) kits after prior preparation of urine and serum samples following the manufacturer's instructions.

    Techniques:

    AKI-marker NGAL serum levels and tissue gene expression of P-Selectin and ICAM-1 as well as NGAL were significantly reduced after IR in mice treated with Bβ 15–42 compared to untreated mice. ( A ) Tissue gene expression of adhesion molecules P-Selectin (left graph) and ICAM-1 (right graph). ( B ) Tissue gene expression of the AKI marker NGAL (left graph). Circulating protein levels of serum NGAL (right graph). Values are expressed in % of NaCl for mRNA analyses and in ng/ml for ELISA measurements. All experiments were performed in duplicates. Error bars represent means ± SEM. *P

    Journal: PLoS ONE

    Article Title: The Small Fibrinopeptide B?15–42 as Renoprotective Agent Preserving the Endothelial and Vascular Integrity in Early Ischemia Reperfusion Injury in the Mouse Kidney

    doi: 10.1371/journal.pone.0084432

    Figure Lengend Snippet: AKI-marker NGAL serum levels and tissue gene expression of P-Selectin and ICAM-1 as well as NGAL were significantly reduced after IR in mice treated with Bβ 15–42 compared to untreated mice. ( A ) Tissue gene expression of adhesion molecules P-Selectin (left graph) and ICAM-1 (right graph). ( B ) Tissue gene expression of the AKI marker NGAL (left graph). Circulating protein levels of serum NGAL (right graph). Values are expressed in % of NaCl for mRNA analyses and in ng/ml for ELISA measurements. All experiments were performed in duplicates. Error bars represent means ± SEM. *P

    Article Snippet: ELISA on mouse NGAL (R & D Systems Inc., Quantikinine® ELISA, USA) was carried out according to the manufacturer’s protocol.

    Techniques: Marker, Expressing, Mouse Assay, Enzyme-linked Immunosorbent Assay

    FGF23 levels rises early after renal injury. Time course data were obtained from time 0, 1, 2, 4, 6, and 8 hours after FA or vehicle injection. N=7-9 for time-points 0-4, and N=3-5 for time points 6 and 8; shown are mean values ±SEM from two independent experiments. *Indicates the earliest time point at which a significant difference between FA (solid line) and vehicle (dotted line) injected animals was observed. A. Plasma BUN levels (mg/dL). B. Plasma phosphate levels (mg/dL). C. Plasma iFGF23 levels (pg/mL). D. Plasma NGAL levels (ng/mL). E. Plasma cFGF23 levels (pg/mL).

    Journal: Kidney international

    Article Title: Plasma FGF23 levels increase rapidly after acute kidney injury

    doi: 10.1038/ki.2013.150

    Figure Lengend Snippet: FGF23 levels rises early after renal injury. Time course data were obtained from time 0, 1, 2, 4, 6, and 8 hours after FA or vehicle injection. N=7-9 for time-points 0-4, and N=3-5 for time points 6 and 8; shown are mean values ±SEM from two independent experiments. *Indicates the earliest time point at which a significant difference between FA (solid line) and vehicle (dotted line) injected animals was observed. A. Plasma BUN levels (mg/dL). B. Plasma phosphate levels (mg/dL). C. Plasma iFGF23 levels (pg/mL). D. Plasma NGAL levels (ng/mL). E. Plasma cFGF23 levels (pg/mL).

    Article Snippet: ELISAs were used for PTH and mouse cFGF23 (Immutopics, San Clemente, CA), iFGF23 (Kainos, Tokyo, Japan), 1,25D (IDS, Fountain Hills, AZ), and plasma NGAL (R & D systems, Minneapolis, MN).

    Techniques: Injection

    In vitro analysis of NGAL expression and release by human tubular epithelial cells (TEC) in different experimental conditions. Relative quantification analysis (A) of NGAL mRNA expression in TEC evaluated by qRT-PCR; mRNA expression of 3 different experiments was normalized for both GAPDH (black columns) and Beta-Actin (white columns) after calculating housekeeping gene variation (see also methods ). Hypoxia and 20 ng/ml Tacrolimus (Tac) significantly increased NGAL mRNA expression when compared to basal culture condition (Control, *p

    Journal: PLoS ONE

    Article Title: Neutrophil Gelatinase Associated Lipocalin Is an Early and Accurate Biomarker of Graft Function and Tissue Regeneration in Kidney Transplantation from Extended Criteria Donors

    doi: 10.1371/journal.pone.0129279

    Figure Lengend Snippet: In vitro analysis of NGAL expression and release by human tubular epithelial cells (TEC) in different experimental conditions. Relative quantification analysis (A) of NGAL mRNA expression in TEC evaluated by qRT-PCR; mRNA expression of 3 different experiments was normalized for both GAPDH (black columns) and Beta-Actin (white columns) after calculating housekeeping gene variation (see also methods ). Hypoxia and 20 ng/ml Tacrolimus (Tac) significantly increased NGAL mRNA expression when compared to basal culture condition (Control, *p

    Article Snippet: To investigate the activity of NGAL on TEC, 500ng/ml of recombinant NGAL (R & D Systems, Minneapolis, MN) were added to cell cultures.

    Techniques: In Vitro, Expressing, Quantitative RT-PCR