Journal: Experimental and Therapeutic Medicine

Article Title: Mesenchymal stem cell-conditioned media suppresses inflammation-associated overproliferation of pulmonary artery smooth muscle cells in a rat model of pulmonary hypertension

doi: 10.3892/etm.2015.2953

Figure Lengend Snippet: Rat gene primers for RT-qPCR.

Article Snippet: After 3 days of co-culture, PASMCs from each of the three groups were sequentially fixed with 4% paraformaldehyde (15 min), incubated with 0.3% Triton X-100 and 1% bovine serum albumin (20 min), and then incubated with mouse anti-rat NFATc2 monoclonal antibody (1:100 dilution; cat. no. NB300-504; Novus Biologicals, LLC, Littleton, CO, USA) overnight at 4°C.

Techniques: Sequencing

Journal: Experimental and Therapeutic Medicine

Article Title: Mesenchymal stem cell-conditioned media suppresses inflammation-associated overproliferation of pulmonary artery smooth muscle cells in a rat model of pulmonary hypertension

doi: 10.3892/etm.2015.2953

Figure Lengend Snippet: Changes in TNF-α, CaN and NFATc2 in the co-culture system. (A) Protein expression levels of TNF-α in the supernatant. (B) Proliferation of PASMCs. mRNA expression of (C) CaN and (D) NFATc2 in PASMCs. (E) CaN activity in PASMCs. Group A, PASMCs alone; Group B, PASMCs + T cells; Group C, PASMCs +T cells + MSC-CM. Data are presented as mean ± standard deviation (n=6). *P<0.01. Activation of NFATc2 in PASMCs in the co-culture system for (F) group A; (G) group B, and (H) group C (fluorescein isothiocyanate/DAPI staining; magnification, ×400). TNF-α, tumor necrosis factor-α; PASMCs, pulmonary artery smooth muscle cells; MSC-CM, mesenchymal stem cell-conditioned media; OD, optical density; CaN, calcineurin; NFATc2, nuclear factor of activated T cells c2.

Article Snippet: After 3 days of co-culture, PASMCs from each of the three groups were sequentially fixed with 4% paraformaldehyde (15 min), incubated with 0.3% Triton X-100 and 1% bovine serum albumin (20 min), and then incubated with mouse anti-rat NFATc2 monoclonal antibody (1:100 dilution; cat. no. NB300-504; Novus Biologicals, LLC, Littleton, CO, USA) overnight at 4°C.

Techniques: Co-Culture Assay, Expressing, Activity Assay, Standard Deviation, Activation Assay, Staining

Journal: Journal of Virology

Article Title: Antigenic Restimulation of Virus-Specific Memory CD8 + T Cells Requires Days of Lytic Protein Accumulation for Maximal Cytotoxic Capacity

doi: 10.1128/JVI.01595-20

Figure Lengend Snippet: TaqMan gene expression assays used in real-time PCR a

Article Snippet: Hs00905451_m1 , NFATC2 , Nuclear factor of activated T cells, cytoplasmic, calcineurin-dependent 2.

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Immunopeptidomics, Membrane, Activation Assay, Binding Assay