Journal: Materials Today Bio

Article Title: Neutrophil membrane-mimicking nanodecoys with intrinsic anti-inflammatory properties alleviate sepsis-induced acute liver injury and lethality in a mouse endotoxemia model

doi: 10.1016/j.mtbio.2022.100244

Figure Lengend Snippet: Construction and characterization of neutrophil membrane-mimicking nanodecoys (NM) and red cell membrane-mimicking nanovesicles (RM). (A) and (B) The Cryo-electron micrograph of NM and RM (scale bar, 100 ​nm). (C) Protein profiles of the neutrophil membrane and NMs assessed by sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE). (D) Western blotting analysis of lymphocyte antigen 6 complex locus G6D (Ly6G) and tumor necrosis factor receptor −1 (TNFR1) in RM and NM. (E) and (F) Average diameter size and zeta potential of NM and RM. (G) The biodistribution of NM in major organs (n ​= ​3, statistical differences between groups were performed by a one-way ANOVA) and fluorescence intensity of liver at 24 ​h was set as 100%. ∗ p ​< ​0.05 compared with the fluorescence intensity of liver at 10 ​min. (H) Blood circulation time of NM (n ​= ​3). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The following antibodies were used: anti-TNFR1 (Sino Biological, Beijing, China), Ly6G (Thermo Fisher Scientific, NY, USA), COX-2 (Proteintech, IL, USA), and β-actin antibody (Sigma-Aldrich, MO, USA).

Techniques: SDS Page, Western Blot, Fluorescence