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Image Search Results
Journal: PLoS ONE
Article Title: Lipid Mixtures Containing a Very High Proportion of Saturated Fatty Acids Only Modestly Impair Insulin Signaling in Cultured Muscle Cells
doi: 10.1371/journal.pone.0120871
Figure Lengend Snippet: Muscle cells were incubated with PALM (open), NORM (filled), or HSFA (hatched). Protein abundance of (A) GPAT1, (B) DGAT1, (C) ATGL, (D) CGI-58, and (E) HSL. In all figure panels data are expressed relative to a no fatty acid control condition (0mM). In panel B, # P <0.05 for a main effect of HSFA vs . PALM and NORM. In panel C, # P <0.05 for a main effect of treatment dose vs . 0mM. Representative blots are inset above each figure panel. GPAT, glycerol-3-phosphate acyltransferase; DGAT, diacylglycerol acyltransferase; ATGL, adipose triacylglycerol lipase; CGI-58, comparative gene identification 58; HSL, hormone sensitive lipase; AU, arbitrary units.
Article Snippet: Various proteins were targeted within whole cell lysates with primary antibodies against: Akt (9272; Cell Signaling Technology, Danvers, MA), pAkt Thr308 (9275; Cell Signaling Technology), GSK3β (9315; Cell Signaling Technology), pGSK3α/β Ser21/9 (9331; Cell Signaling Technology), AS160 (ABS54; EMD Millipore, Billerica, MA), pAS160 Thr642 (3028 P1; Symansis, Auckland, New Zealand),
Techniques: Incubation, Quantitative Proteomics, Control