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<t>Netrin-1</t> and its dependence receptors are upregulated upon Doxorubicin treatment. Source data is available for this figure in the Supporting Information.
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<t>Netrin-1</t> and its dependence receptors are upregulated upon Doxorubicin treatment. Source data is available for this figure in the Supporting Information.
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<t>Netrin-1</t> and its dependence receptors are upregulated upon Doxorubicin treatment. Source data is available for this figure in the Supporting Information.
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<t>Netrin-1</t> and its dependence receptors are upregulated upon Doxorubicin treatment. Source data is available for this figure in the Supporting Information.
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<t>Netrin-1</t> and its dependence receptors are upregulated upon Doxorubicin treatment. Source data is available for this figure in the Supporting Information.
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<t>Netrin-1</t> and its dependence receptors are upregulated upon Doxorubicin treatment. Source data is available for this figure in the Supporting Information.
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Image Search Results


Netrin-1 and its dependence receptors are upregulated upon Doxorubicin treatment. Source data is available for this figure in the Supporting Information.

Journal: EMBO Molecular Medicine

Article Title: Combining chemotherapeutic agents and netrin-1 interference potentiates cancer cell death

doi: 10.1002/emmm.201302654

Figure Lengend Snippet: Netrin-1 and its dependence receptors are upregulated upon Doxorubicin treatment. Source data is available for this figure in the Supporting Information.

Article Snippet: Endogenous netrin-1 was stained using rat monoclonal α-netrin-1 antibody (R&D systems) and Alexa-488 Donkey anti-rat IgG (Molecular probes). p53 protein was stained using the mouse monoclonal α-p53-DO-1 antibody.

Techniques:

Netrin-1 and its receptors expression is increased in several cancer cell lines and in ovarian tumours upon treatment with cytotoxic drugs.

Journal: EMBO Molecular Medicine

Article Title: Combining chemotherapeutic agents and netrin-1 interference potentiates cancer cell death

doi: 10.1002/emmm.201302654

Figure Lengend Snippet: Netrin-1 and its receptors expression is increased in several cancer cell lines and in ovarian tumours upon treatment with cytotoxic drugs.

Article Snippet: Endogenous netrin-1 was stained using rat monoclonal α-netrin-1 antibody (R&D systems) and Alexa-488 Donkey anti-rat IgG (Molecular probes). p53 protein was stained using the mouse monoclonal α-p53-DO-1 antibody.

Techniques: Expressing

Netrin-1 silencing sensitizes A549R cells to Doxorubicin and induces apoptotic cell death via UNC5B receptor.

Journal: EMBO Molecular Medicine

Article Title: Combining chemotherapeutic agents and netrin-1 interference potentiates cancer cell death

doi: 10.1002/emmm.201302654

Figure Lengend Snippet: Netrin-1 silencing sensitizes A549R cells to Doxorubicin and induces apoptotic cell death via UNC5B receptor.

Article Snippet: Endogenous netrin-1 was stained using rat monoclonal α-netrin-1 antibody (R&D systems) and Alexa-488 Donkey anti-rat IgG (Molecular probes). p53 protein was stained using the mouse monoclonal α-p53-DO-1 antibody.

Techniques:

Interference to netrin-1 and its receptors interaction sensitizes tumour cells to cytotoxic drugs.

Journal: EMBO Molecular Medicine

Article Title: Combining chemotherapeutic agents and netrin-1 interference potentiates cancer cell death

doi: 10.1002/emmm.201302654

Figure Lengend Snippet: Interference to netrin-1 and its receptors interaction sensitizes tumour cells to cytotoxic drugs.

Article Snippet: Endogenous netrin-1 was stained using rat monoclonal α-netrin-1 antibody (R&D systems) and Alexa-488 Donkey anti-rat IgG (Molecular probes). p53 protein was stained using the mouse monoclonal α-p53-DO-1 antibody.

Techniques:

Tumour growth inhibiting effect of combining netrin-1 interference and Doxorubicin.

Journal: EMBO Molecular Medicine

Article Title: Combining chemotherapeutic agents and netrin-1 interference potentiates cancer cell death

doi: 10.1002/emmm.201302654

Figure Lengend Snippet: Tumour growth inhibiting effect of combining netrin-1 interference and Doxorubicin.

Article Snippet: Endogenous netrin-1 was stained using rat monoclonal α-netrin-1 antibody (R&D systems) and Alexa-488 Donkey anti-rat IgG (Molecular probes). p53 protein was stained using the mouse monoclonal α-p53-DO-1 antibody.

Techniques:

Netrin-1 upregulation is p53-dependent.

Journal: EMBO Molecular Medicine

Article Title: Combining chemotherapeutic agents and netrin-1 interference potentiates cancer cell death

doi: 10.1002/emmm.201302654

Figure Lengend Snippet: Netrin-1 upregulation is p53-dependent.

Article Snippet: Endogenous netrin-1 was stained using rat monoclonal α-netrin-1 antibody (R&D systems) and Alexa-488 Donkey anti-rat IgG (Molecular probes). p53 protein was stained using the mouse monoclonal α-p53-DO-1 antibody.

Techniques:

Resources and reagents used

Journal: The Journal of Neuroscience

Article Title: Somatotopy of Mouse Spinothalamic Innervation and the Localization of a Noxious Stimulus Requires Deleted in Colorectal Carcinoma Expression by Phox2a Neurons

doi: 10.1523/JNEUROSCI.1164-22.2022

Figure Lengend Snippet: Resources and reagents used

Article Snippet: Reagent or resource Source/reference Identifier Mice (MGI notation) Dcc flox Krimpenfort et al. 2012 MGI: 3665466 Phox2a Cre Roome et al. 2022 RRID: NA Ai14 B6;129S6-Gt (ROSA)26Sortm14(CAG- tdTomato ) Hze/J The Jackson Laboratory Catalog #JAX:007908 RRID: IMSR_JAX:007908 Cdx2 FlpO Tg(CDX2-flpo)#Gld Dr. Martyn Goulding, Salk Institute, San Diego MGI: 5911680 RRID: NA Ai65 B6;129S-Gt ROSA)26Sortm65.1(CAG- dTomato) Hze/J The Jackson Laboratory Catalog #JAX:021875 RRID: IMSR_JAX:021875 Ntn1 Bgeo Skarnes et al., 1995 RRID: NA 129S1/SvImJ The Jackson Laboratory Catalog #JAX:002448 RRID: IMSR_JAX:002448 B6C3F1/J The Jackson Laboratory Catalog #JAX: 100010 RRID: IMSR_JAX:100010 Primary antibodies Goat anti-mouse DCC (1:500) R&D Systems Catalog #AF844 RRID: AB_2089765 Rabbit anti-Phox2a (1:10,000 from frozen stock) Jean-François Brunet (École Normale Supérieure, Paris) ( Tiveron et al., 1996 ) RRID: AB_2315159 Rabbit anti-RFP (red fluorescent protein; 1:1000) Rockland Catalog #600-401-379 RRID: AB_2209751 Goat anti-tdT (red fluorescent protein; 1:300) SICGEN Catalog #AB8181-200 RRID: AB_2722750 Rabbit anti-GFP (1:1000) Life Technologies Catalog #A-11122 RRID: AB_221569 Goat anti-netrin-1 (1:200) R&D Systems Catalog #AF1109, RRID: AB_2298775 Rabbit anti-c- fos (1:500) Cell Signaling Technology Catalog #2250S RRID: AB_2247211 Rabbit anti-Pax6 (1:500) Millipore Catalog #AB2237, RRID: AB_1587367 Rabbit anti-Sox2 (1:200) Abcam Catalog #97959 RRID: AB_2341193 Secondary antibodies Alexa 488 Donkey anti-Rabbit (1:500) Jackson ImmunoResearch Laboratories Catalog #711–545-152 Lot #141848 RRID: AB_2313584 Alexa 488 Donkey anti-Goat (1:500) Jackson ImmunoResearch Laboratories Catalog #705–545-147 Lot #136089 RRID: AB_2336933 Cy3 Donkey anti-Rabbit (1:500) Jackson ImmunoResearch Laboratories Catalog #711–165-152 Lot #138270 RRID: AB_2307443 Cy3 Donkey anti-Goat (1:500) Jackson ImmunoResearch Laboratories Catalog #705–165-147 Lot #134527 RRID: AB_2307351 Cy5 Donkey anti-Rabbit (1:500) Jackson ImmunoResearch Laboratories Catalog #711–175-152 Lot #138336 RRID: AB_2340607 Cy5 Donkey anti-Goat (1:500) Jackson ImmunoResearch Laboratories Catalog #: 705–175-147 Lot#: 134531 RRID: AB_2340415 Chemicals ( E )-Capsaicin Tocris Bioscience Catalog #0462 Lot #7A/218361 RRID: NA 4′,6-diamidino-2-phenylindole (DAPI) Thermo Fisher Scientific Catalog #D1306 RRID: NA Alexa 488-conjugated Choleratoxin B Thermo Fisher Scientific Catalog # C22841 Lot #2038245 RRID: NA X-gal Millipore Sigma Catalog #7240-90-6 RRID: NA MOWIOL Millipore Sigma Catalog #81381 RRID: NA Open in a separate window MGI, Mouse Genome Informatics; NA, Not applicable.

Techniques: