Journal: Marine Drugs

Article Title: Mannose-Specific Lectins from Marine Algae: Diverse Structural Scaffolds Associated to Common Virucidal and Anti-Cancer Properties

doi: 10.3390/md17080440

Figure Lengend Snippet: Overview of algal lectins with a mannose-specificity. OAAH— Oscillatoria agardhii agglutinin homolog; GNA—Galanthus nivalis agglutinin.

Article Snippet: An unrooted phylogenetic tree was built up from the multiple alignment of jacalin-related lectins using the uncorrected neighbor joining method integrated in MacVector.

Techniques: Algae

Journal: Marine Drugs

Article Title: Mannose-Specific Lectins from Marine Algae: Diverse Structural Scaffolds Associated to Common Virucidal and Anti-Cancer Properties

doi: 10.3390/md17080440

Figure Lengend Snippet: Structural diversity of the mannose-binding seaweed lectins. ( A , B ). Lateral ( A ) and front ( B ) views of the ribbon diagram of griffithsin monomer ( protein data bank (PDB) code 1LL2) in complex with a pentamannoside (colored cyan), showing the β-prism-I organization of strands of β-sheet. Surface electrostatic potential (electronegatively and electropositively charged surfaces colored in red and blue, respectively; neutral surfaces colored grey) is shown in transparency. ( C , D ). Lateral ( C ) and front ( D ) views of the ribbon diagram of the modelled Agardhiella subulata lectin ASL-1, in complex with a pentamannoside (colored cyan), showing the β-barrel organization of strands of β-sheet. Surface electrostatic potential is shown in transparency. ( E , F ). Lateral ( E ) and front ( F ) views of the ribbon diagram of the modelled lectin from Ostreococcus tauri OtL, in complex with a dimannoside (colored cyan), showing the β-sandwich organization of strands of β-sheet. Surface electrostatic potential is shown in transparency. ASL-1 and OtL lectins were modelled with YASARA, and rendered with Chimera.

Article Snippet: An unrooted phylogenetic tree was built up from the multiple alignment of jacalin-related lectins using the uncorrected neighbor joining method integrated in MacVector.

Techniques: Binding Assay

Journal: Marine Drugs

Article Title: Mannose-Specific Lectins from Marine Algae: Diverse Structural Scaffolds Associated to Common Virucidal and Anti-Cancer Properties

doi: 10.3390/md17080440

Figure Lengend Snippet: PDB codes of lectins from seaweeds, complexed to mannose, glucose, N -acetyl-glucosamine, maltose and high-mannose glycans.

Article Snippet: An unrooted phylogenetic tree was built up from the multiple alignment of jacalin-related lectins using the uncorrected neighbor joining method integrated in MacVector.

Techniques:

Journal: Marine Drugs

Article Title: Mannose-Specific Lectins from Marine Algae: Diverse Structural Scaffolds Associated to Common Virucidal and Anti-Cancer Properties

doi: 10.3390/md17080440

Figure Lengend Snippet: Multiple amino acid sequence alignment of griffithsin and jacalin-related lectins from higher plants (jacalin and artocarpin from Artocarpus integrifolia , frutalin from Artocarpus incisa , and Morniga-M from Morus nigra ). Griffithsin shares 25% identity and 70% homology with jacalin-related lectins from higher plants. Sequence alignment was performed with CLUSTAL-X.

Article Snippet: An unrooted phylogenetic tree was built up from the multiple alignment of jacalin-related lectins using the uncorrected neighbor joining method integrated in MacVector.

Techniques: Sequencing

Journal: Marine Drugs

Article Title: Mannose-Specific Lectins from Marine Algae: Diverse Structural Scaffolds Associated to Common Virucidal and Anti-Cancer Properties

doi: 10.3390/md17080440

Figure Lengend Snippet: Phylogenetic tree built from the multiple amino acid sequence alignment of jacalin-related lectins from red algae (red boxes), plants (green box), fungi (orange boxes), and bacteria (blue boxes). Lectin of the green alga Volvox carteri is green boxed, and lectins from unicellular ciliates are not boxed.

Article Snippet: An unrooted phylogenetic tree was built up from the multiple alignment of jacalin-related lectins using the uncorrected neighbor joining method integrated in MacVector.

Techniques: Sequencing, Algae, Bacteria

Journal: Marine Drugs

Article Title: Mannose-Specific Lectins from Marine Algae: Diverse Structural Scaffolds Associated to Common Virucidal and Anti-Cancer Properties

doi: 10.3390/md17080440

Figure Lengend Snippet: Three-dimensional structure of gp120 complexed to a CD4 molecule (PDB code 5FYK). The high-mannose N -glycan chain decorating gp120 are rendered as cyan colored sticks. Lectins which specifically bind to the high-mannose N -glycans exposed at the surface of gp120, interfere with the recognition of gp120 by the coreceptors of the CD4+ T lymphocytes.

Article Snippet: An unrooted phylogenetic tree was built up from the multiple alignment of jacalin-related lectins using the uncorrected neighbor joining method integrated in MacVector.

Techniques: Glycoproteomics

Journal: Marine Drugs

Article Title: Mannose-Specific Lectins from Marine Algae: Diverse Structural Scaffolds Associated to Common Virucidal and Anti-Cancer Properties

doi: 10.3390/md17080440

Figure Lengend Snippet: List of the man-specific seaweed lectins inhibiting HIV infection by binding to the viral gp120 envelope protein. EC 50 is in the range between 0.1 nM and 1.8 nM, depending on differences in methods used to quantify the anti-HIV activity of griffithsin in vitro. IC 50 is usually less than 1.0 nM.

Article Snippet: An unrooted phylogenetic tree was built up from the multiple alignment of jacalin-related lectins using the uncorrected neighbor joining method integrated in MacVector.

Techniques: Infection, Binding Assay, Activity Assay, In Vitro, Algae

Journal: Marine Drugs

Article Title: Mannose-Specific Lectins from Marine Algae: Diverse Structural Scaffolds Associated to Common Virucidal and Anti-Cancer Properties

doi: 10.3390/md17080440

Figure Lengend Snippet: Cytotoxic effects of Man-specific lectins on cancer cells (reported during the last decade). Apopt.: apoptosis.

Article Snippet: An unrooted phylogenetic tree was built up from the multiple alignment of jacalin-related lectins using the uncorrected neighbor joining method integrated in MacVector.

Techniques: Algae

Journal: Marine Drugs

Article Title: Mannose-Specific Lectins from Marine Algae: Diverse Structural Scaffolds Associated to Common Virucidal and Anti-Cancer Properties

doi: 10.3390/md17080440

Figure Lengend Snippet: Other investigated biomedical properties of Man-specific seaweed lectins.

Article Snippet: An unrooted phylogenetic tree was built up from the multiple alignment of jacalin-related lectins using the uncorrected neighbor joining method integrated in MacVector.

Techniques: Algae