Journal: Experimental neurology

Article Title: Cathepsin B knockout confers significant brain protection in the mouse model of stroke.

doi: 10.1016/j.expneurol.2023.114499

Figure Lengend Snippet: Fig. 1. A: Schematic middle cerebral artery (MCA) occlusion (MCAO) procedures. A Laser Doppler Probe was affixed to the exposed temporal bone surface to record the MCA blood flow during the entire peri-MCAO periods. A monofilament occluder was inserted via an incision of the external carotid artery (ECA), gone through the internal carotid artery (ICA), and advanced to the base of the MCA. B: An example of Laser Doppler Flowmetry of the blood flow in the MCA region. The MCA blood flow was reduced: (i) by 20–50% after temporary clamping of the common carotid artery (CCAO), and (ii) by >90% after MCAO. After monofilament removal, MCA blood flow returns to above 60% of pre-ischemic level, which was used as a confirmation of reperfusion. C: The MCA blood flow was continuously monitored during the peri-MCAO periods in all mice used in this study.

Article Snippet: Briefly, the middle cerebral artery blood flow was monitored via a small incision to affix a needle laser Doppler flowmetry (LDF) probe (MNP100XP, ADInstruments, CO, USA) to the temporal bone surface (Fig. 1A).

Techniques: