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Image Search Results
Journal: PLoS Pathogens
Article Title: Bacterial Pathogens Activate a Common Inflammatory Pathway through IFNλ Regulation of PDCD4
doi: 10.1371/journal.ppat.1003682
Figure Lengend Snippet: (A) qRT-PCR analysis of IFNλ mRNA in BMDCs stimulated with heat killed USA300, µ ± SD. (B) ELISA analysis of IFNλ in BAL of WT mice following 4 and 18 hours of infection with USA300. (C) Numbers (CFU) of USA300 recovered from BAL of WT and IL-28R −/− mice following 4 and 18 hours of infection. (D) Numbers (CFU) of USA300 recovered from lung tissue of WT and IL-28R −/− mice following 4 and 18 hours of infection. (E) Trichrome stained lungs from WT and IL-28R −/− mice following and 18 hour USA300 infection (original magnification 100x, insert 400x). (F) Numbers of dendritic cells (DC), macrophages (MAC), and neutrophils in BAL of WT and IL-28R −/− in unstimulated (NS) mice or following 4 or 18 hours of infection. (G) Numbers of dendritic cells (DC), macrophages (MAC), and neutrophils in lung tissue of WT and IL-28R −/− in unstimulated (NS) mice or following 4 or 18 hours of infection. Data are representative of at least 2 independent experiments.
Article Snippet: For miRNA analysis cDNA was generated and qRT-PCR was run using NCode miRNA First-Strand cDNA Synthesis and
Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Infection, Staining
Journal: PLoS Pathogens
Article Title: Bacterial Pathogens Activate a Common Inflammatory Pathway through IFNλ Regulation of PDCD4
doi: 10.1371/journal.ppat.1003682
Figure Lengend Snippet: (A) qRT-PCR analysis of IL-8 in 16HBE cells treated with control or PDCD4 siRNA and infected with USA300, µ ± SD. (B) qRT-PCR analysis of miR-21 in 16HBE cells treated with recombinant IFNλ for 1 hour, µ ± SD. (C) Western blot analysis of PDCD4 in 16HBE cells treated with recombinant IFNλ. (D) qRT-PCR analysis of miR-21 in 16HBE cells pretreated with STAT3 inhibitor or DMSO treated with recombinant IFNλ for 1 hour, µ ± SD. (E) Western blot analysis of PDCD4 in 16HBE cells pretreated with STAT3 inhibitor or DMSO treated with recombinant IFNλ for 1 hour. Lysate from 16HBE cells treated with DMSO and IFNλ was used as a positive control (pos). (F) Western blot analysis of PDCD4 in human epithelial cells (16HBE) following stimulation with S. aureus protein A (SpA), µ ± SD. (G) Western blot analysis of PDCD4 in 16HBE cells treated with anti-TNFR1 or control IgG and SPA for 2 hours, µ ± SD. Data are representative of at least 2 independent experiments.
Article Snippet: For miRNA analysis cDNA was generated and qRT-PCR was run using NCode miRNA First-Strand cDNA Synthesis and
Techniques: Quantitative RT-PCR, Infection, Recombinant, Western Blot, Positive Control
Journal: PLoS Pathogens
Article Title: Bacterial Pathogens Activate a Common Inflammatory Pathway through IFNλ Regulation of PDCD4
doi: 10.1371/journal.ppat.1003682
Figure Lengend Snippet: (B) qRT-PCR analysis of PDCD4 mRNA in the lungs of WT and IL-28R −/− mice following infection with USA300, µ ± SD. (C) Western blot analysis of PDCD4 in the lungs of WT and IL-28R −/− mice following infection with USA300, µ ± SD. (D) Numbers (CFU) of USA300 recovered from BAL of WT and PDCD4 −/− mice following 18 hours of infection. (E) Numbers (CFU) of USA300 recovered from lung tissue of WT and PDCD4 −/− mice following 18 hours of infection. (F) Trichrome stained lungs from WT and PDCD4 −/− mice following and 18 hour USA300 infection (original magnification 100x, insert 400x). (G) ELISA analysis of individual cytokines in BAL of WT and PDCD4 −/− mice. Data are representative of at least 2 independent experiments.
Article Snippet: For miRNA analysis cDNA was generated and qRT-PCR was run using NCode miRNA First-Strand cDNA Synthesis and
Techniques: Quantitative RT-PCR, Infection, Western Blot, Staining, Enzyme-linked Immunosorbent Assay
Journal: PLoS Pathogens
Article Title: Bacterial Pathogens Activate a Common Inflammatory Pathway through IFNλ Regulation of PDCD4
doi: 10.1371/journal.ppat.1003682
Figure Lengend Snippet: (A) ELISA analysis of individual cytokines in BAL of WT and IL-28R −/− mice. (B) Western blot analysis of MX1 expression in lungs of WT and IL-28R −/− mice following 4 and 18 hours of infection with PAK. (C) qRT-PCR analysis of IL-8 in 16HBE cells treated with control or PDCD4 siRNA and infected with PAK, µ ± SD. (D) qRT-PCR analysis of miR-21 in the lungs of WT and IL-28R −/− mice following infection with PAK, µ ± SD. (E) qRT-PCR analysis of PDCD4 mRNA in the lungs of WT and IL-28R −/− mice following infection with PAK, µ ± SD. (F) Western blot analysis of PDCD4 in the lungs of WT and IL-28R −/− mice following infection with PAK, µ ± SD. (G) Western blot analysis of phosphorylation of p70S6K in 16HBE cells treated with recombinant IFNλ. (H) Western blot analysis of phosphorylation of p70S6K and PDCD4 in the lungs of WT and IL-28R −/− mice following infection with PAK. (I) Western blot analysis of phosphorylation of p70S6K and PDCD4 in the lungs of WT and IL-28R −/− mice following infection with USA300. Data are representative of at least 2 independent experiments.
Article Snippet: For miRNA analysis cDNA was generated and qRT-PCR was run using NCode miRNA First-Strand cDNA Synthesis and
Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Infection, Quantitative RT-PCR, Recombinant