ncbi reference sequence database (refseq) Search Results


bla oxa-14  (SmartGene GmbH)
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Bla Oxa 14, supplied by SmartGene GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lsaldh16  (GenScript corporation)
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falco peregrinus bcma  (FALCO Biosystems Ltd)
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gene exp bcl2 hs00153350 m1  (Thermo Fisher)
97
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genes encoding α-ketoisocaproate dioxygenase from rattus norvegicus (rnkicd, ncbi reference sequence: np_058929.1)  (GenScript corporation)
90
GenScript corporation genes encoding α-ketoisocaproate dioxygenase from rattus norvegicus (rnkicd, ncbi reference sequence: np_058929.1)
Genes Encoding α Ketoisocaproate Dioxygenase From Rattus Norvegicus (Rnkicd, Ncbi Reference Sequence: Np 058929.1), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genes encoding α-ketoisocaproate dioxygenase from rattus norvegicus (rnkicd, ncbi reference sequence: np_058929.1) - by Bioz Stars, 2026-08
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hgxprt gene for hypoxanthine-guanine-xanthine phosphoribosyltransferase from thermus thermophilus hb8  (GenScript corporation)
90
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Hgxprt Gene For Hypoxanthine Guanine Xanthine Phosphoribosyltransferase From Thermus Thermophilus Hb8, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fusobacterium nucleatum subsp polymorphum strain f0401  (ATCC)
94
ATCC fusobacterium nucleatum subsp polymorphum strain f0401
Fusobacterium Nucleatum Subsp Polymorphum Strain F0401, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene trpc4dr  (GenScript corporation)
90
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Gene Trpc4dr, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mettl3 coding sequence  (Sangon Biotech)
86
Sangon Biotech mettl3 coding sequence
( A) Details of seven potential methylation modification sites on circSIK2. (B ) The relative expression of circSIK2, <t>METTL3,</t> and SIK2 mRNA in myoblast cells after transfected with pCD25-circSIK2 + pCD3.1, pCD25-circSIK2 + pCD3.1-METTL3, and pCD25 + pCD3.1. ( C) Upper: the expression of SIK2-176aa-flag after transfected with pCD3.1-SIK2-176aa-flag and pCD3.1-SIK2-176aa-flag + pCD3.1-METTL3. Lower: the expression of SIK2-176aa after transfected with pCD3.1-SIK2-176aa-flag, pCD25-circSIK2, and pCD25-circSIK2 + pCD3.1-METTL3. (D ) Dual-luciferase reporter assay using the different regions of the circSIK2 after co-transfected with METTL3 in DF-1 cells. ( E) Upper: Dual-luciferase reporter assay using the 151–200 fragment after co-transfected with METTL3 in DF-1 cells. Under: Dual-luciferase reporter assay after 131A-WT and 131A-MT fragment was co-transfected with METTL14 in DF-1 cells. (F ) Amplification curve and qPCR CT value in circSIK2 131 m 6 A site and 125 A site after METTL3 overexpression in myoblast cells. ( G, H) circSIK2 pulldown image ( G ) (OV = pCD25-circSIK2, NC = pCD25) and binding proteins’ string network ( H ) screened by mass spectrometry in myoblast cells. ( I ) circSIK2 pulldown results confirmed by western blot with HNRNPA2B1 antibody in myoblast cells. (J) The expression pattern of METTL3, METTL14, EIF3A, and EIF3F in different stages of myoblast differentiation. GM (growth media; n = 3) stands for myoblasts in the proliferative phase. DM (differentiation media; n = 3), DM1-DM6 means differentiation from day 1 to day 6.
Mettl3 Coding Sequence, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reference sequence database  (Biotechnology Information)
86
Biotechnology Information reference sequence database
( A) Details of seven potential methylation modification sites on circSIK2. (B ) The relative expression of circSIK2, <t>METTL3,</t> and SIK2 mRNA in myoblast cells after transfected with pCD25-circSIK2 + pCD3.1, pCD25-circSIK2 + pCD3.1-METTL3, and pCD25 + pCD3.1. ( C) Upper: the expression of SIK2-176aa-flag after transfected with pCD3.1-SIK2-176aa-flag and pCD3.1-SIK2-176aa-flag + pCD3.1-METTL3. Lower: the expression of SIK2-176aa after transfected with pCD3.1-SIK2-176aa-flag, pCD25-circSIK2, and pCD25-circSIK2 + pCD3.1-METTL3. (D ) Dual-luciferase reporter assay using the different regions of the circSIK2 after co-transfected with METTL3 in DF-1 cells. ( E) Upper: Dual-luciferase reporter assay using the 151–200 fragment after co-transfected with METTL3 in DF-1 cells. Under: Dual-luciferase reporter assay after 131A-WT and 131A-MT fragment was co-transfected with METTL14 in DF-1 cells. (F ) Amplification curve and qPCR CT value in circSIK2 131 m 6 A site and 125 A site after METTL3 overexpression in myoblast cells. ( G, H) circSIK2 pulldown image ( G ) (OV = pCD25-circSIK2, NC = pCD25) and binding proteins’ string network ( H ) screened by mass spectrometry in myoblast cells. ( I ) circSIK2 pulldown results confirmed by western blot with HNRNPA2B1 antibody in myoblast cells. (J) The expression pattern of METTL3, METTL14, EIF3A, and EIF3F in different stages of myoblast differentiation. GM (growth media; n = 3) stands for myoblasts in the proliferative phase. DM (differentiation media; n = 3), DM1-DM6 means differentiation from day 1 to day 6.
Reference Sequence Database, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotechnology information ncbi refseq  (Biotechnology Information)
86
Biotechnology Information biotechnology information ncbi refseq
( A) Details of seven potential methylation modification sites on circSIK2. (B ) The relative expression of circSIK2, <t>METTL3,</t> and SIK2 mRNA in myoblast cells after transfected with pCD25-circSIK2 + pCD3.1, pCD25-circSIK2 + pCD3.1-METTL3, and pCD25 + pCD3.1. ( C) Upper: the expression of SIK2-176aa-flag after transfected with pCD3.1-SIK2-176aa-flag and pCD3.1-SIK2-176aa-flag + pCD3.1-METTL3. Lower: the expression of SIK2-176aa after transfected with pCD3.1-SIK2-176aa-flag, pCD25-circSIK2, and pCD25-circSIK2 + pCD3.1-METTL3. (D ) Dual-luciferase reporter assay using the different regions of the circSIK2 after co-transfected with METTL3 in DF-1 cells. ( E) Upper: Dual-luciferase reporter assay using the 151–200 fragment after co-transfected with METTL3 in DF-1 cells. Under: Dual-luciferase reporter assay after 131A-WT and 131A-MT fragment was co-transfected with METTL14 in DF-1 cells. (F ) Amplification curve and qPCR CT value in circSIK2 131 m 6 A site and 125 A site after METTL3 overexpression in myoblast cells. ( G, H) circSIK2 pulldown image ( G ) (OV = pCD25-circSIK2, NC = pCD25) and binding proteins’ string network ( H ) screened by mass spectrometry in myoblast cells. ( I ) circSIK2 pulldown results confirmed by western blot with HNRNPA2B1 antibody in myoblast cells. (J) The expression pattern of METTL3, METTL14, EIF3A, and EIF3F in different stages of myoblast differentiation. GM (growth media; n = 3) stands for myoblasts in the proliferative phase. DM (differentiation media; n = 3), DM1-DM6 means differentiation from day 1 to day 6.
Biotechnology Information Ncbi Refseq, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ncbi serratia sp  (ATCC)
96
ATCC ncbi serratia sp
( A) Details of seven potential methylation modification sites on circSIK2. (B ) The relative expression of circSIK2, <t>METTL3,</t> and SIK2 mRNA in myoblast cells after transfected with pCD25-circSIK2 + pCD3.1, pCD25-circSIK2 + pCD3.1-METTL3, and pCD25 + pCD3.1. ( C) Upper: the expression of SIK2-176aa-flag after transfected with pCD3.1-SIK2-176aa-flag and pCD3.1-SIK2-176aa-flag + pCD3.1-METTL3. Lower: the expression of SIK2-176aa after transfected with pCD3.1-SIK2-176aa-flag, pCD25-circSIK2, and pCD25-circSIK2 + pCD3.1-METTL3. (D ) Dual-luciferase reporter assay using the different regions of the circSIK2 after co-transfected with METTL3 in DF-1 cells. ( E) Upper: Dual-luciferase reporter assay using the 151–200 fragment after co-transfected with METTL3 in DF-1 cells. Under: Dual-luciferase reporter assay after 131A-WT and 131A-MT fragment was co-transfected with METTL14 in DF-1 cells. (F ) Amplification curve and qPCR CT value in circSIK2 131 m 6 A site and 125 A site after METTL3 overexpression in myoblast cells. ( G, H) circSIK2 pulldown image ( G ) (OV = pCD25-circSIK2, NC = pCD25) and binding proteins’ string network ( H ) screened by mass spectrometry in myoblast cells. ( I ) circSIK2 pulldown results confirmed by western blot with HNRNPA2B1 antibody in myoblast cells. (J) The expression pattern of METTL3, METTL14, EIF3A, and EIF3F in different stages of myoblast differentiation. GM (growth media; n = 3) stands for myoblasts in the proliferative phase. DM (differentiation media; n = 3), DM1-DM6 means differentiation from day 1 to day 6.
Ncbi Serratia Sp, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A) Details of seven potential methylation modification sites on circSIK2. (B ) The relative expression of circSIK2, METTL3, and SIK2 mRNA in myoblast cells after transfected with pCD25-circSIK2 + pCD3.1, pCD25-circSIK2 + pCD3.1-METTL3, and pCD25 + pCD3.1. ( C) Upper: the expression of SIK2-176aa-flag after transfected with pCD3.1-SIK2-176aa-flag and pCD3.1-SIK2-176aa-flag + pCD3.1-METTL3. Lower: the expression of SIK2-176aa after transfected with pCD3.1-SIK2-176aa-flag, pCD25-circSIK2, and pCD25-circSIK2 + pCD3.1-METTL3. (D ) Dual-luciferase reporter assay using the different regions of the circSIK2 after co-transfected with METTL3 in DF-1 cells. ( E) Upper: Dual-luciferase reporter assay using the 151–200 fragment after co-transfected with METTL3 in DF-1 cells. Under: Dual-luciferase reporter assay after 131A-WT and 131A-MT fragment was co-transfected with METTL14 in DF-1 cells. (F ) Amplification curve and qPCR CT value in circSIK2 131 m 6 A site and 125 A site after METTL3 overexpression in myoblast cells. ( G, H) circSIK2 pulldown image ( G ) (OV = pCD25-circSIK2, NC = pCD25) and binding proteins’ string network ( H ) screened by mass spectrometry in myoblast cells. ( I ) circSIK2 pulldown results confirmed by western blot with HNRNPA2B1 antibody in myoblast cells. (J) The expression pattern of METTL3, METTL14, EIF3A, and EIF3F in different stages of myoblast differentiation. GM (growth media; n = 3) stands for myoblasts in the proliferative phase. DM (differentiation media; n = 3), DM1-DM6 means differentiation from day 1 to day 6.

Journal: PLOS Genetics

Article Title: METTL3 facilitates the translation of CircSIK2 during chicken myogenesis in an m 6 A dependent manner

doi: 10.1371/journal.pgen.1011934

Figure Lengend Snippet: ( A) Details of seven potential methylation modification sites on circSIK2. (B ) The relative expression of circSIK2, METTL3, and SIK2 mRNA in myoblast cells after transfected with pCD25-circSIK2 + pCD3.1, pCD25-circSIK2 + pCD3.1-METTL3, and pCD25 + pCD3.1. ( C) Upper: the expression of SIK2-176aa-flag after transfected with pCD3.1-SIK2-176aa-flag and pCD3.1-SIK2-176aa-flag + pCD3.1-METTL3. Lower: the expression of SIK2-176aa after transfected with pCD3.1-SIK2-176aa-flag, pCD25-circSIK2, and pCD25-circSIK2 + pCD3.1-METTL3. (D ) Dual-luciferase reporter assay using the different regions of the circSIK2 after co-transfected with METTL3 in DF-1 cells. ( E) Upper: Dual-luciferase reporter assay using the 151–200 fragment after co-transfected with METTL3 in DF-1 cells. Under: Dual-luciferase reporter assay after 131A-WT and 131A-MT fragment was co-transfected with METTL14 in DF-1 cells. (F ) Amplification curve and qPCR CT value in circSIK2 131 m 6 A site and 125 A site after METTL3 overexpression in myoblast cells. ( G, H) circSIK2 pulldown image ( G ) (OV = pCD25-circSIK2, NC = pCD25) and binding proteins’ string network ( H ) screened by mass spectrometry in myoblast cells. ( I ) circSIK2 pulldown results confirmed by western blot with HNRNPA2B1 antibody in myoblast cells. (J) The expression pattern of METTL3, METTL14, EIF3A, and EIF3F in different stages of myoblast differentiation. GM (growth media; n = 3) stands for myoblasts in the proliferative phase. DM (differentiation media; n = 3), DM1-DM6 means differentiation from day 1 to day 6.

Article Snippet: The full length of the METTL3 coding sequence (NCBI Reference Sequence: XM_040655036.2 ) was also synthesized by Sangon Biotech and cloned into the pcDNA3.1 (+) vector.

Techniques: Methylation, Modification, Expressing, Transfection, Luciferase, Reporter Assay, Amplification, Over Expression, Binding Assay, Mass Spectrometry, Western Blot