ncaas Search Results


ncaas  (PepTech Corporation)
90
PepTech Corporation ncaas
<t>Permissivity</t> profiles of the G2 and F9 synthetases. Reported is the amount of sfGFP with each of the <t>ncAAs</t> listed in Figure 1 incorporated at position 150. For each expression, ncAA concentration was 1 mM, except for Tco-amF (3) which was 5 mM because preparations of it consisted of an approximately equal mixture of four stereoisomers and only at these elevated concentrations did sfGFP production became significantly higher than background. Fidelity of each synthetase variant was determined by the amount of sfGFP produced from the same amber stop codon disrupted expression construct but in the absence of any ncAA in the media (indicated by the ‘-ncAA’ bar). For reference, native sfGFP without an amber stop codon expresses at ∼800-1000 mg per liter culture under identical conditions. Error bars represent the standard deviation of sfGFP produced from three independent expression cultures.
Ncaas, supplied by PepTech Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ncaas/pmc04199100-381-10-18?v=PepTech+Corporation
Average 90 stars, based on 1 article reviews
ncaas - by Bioz Stars, 2026-08
90/100 stars
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ncaas  (Rocha labs)
90
Rocha labs ncaas
<t>Permissivity</t> profiles of the G2 and F9 synthetases. Reported is the amount of sfGFP with each of the <t>ncAAs</t> listed in Figure 1 incorporated at position 150. For each expression, ncAA concentration was 1 mM, except for Tco-amF (3) which was 5 mM because preparations of it consisted of an approximately equal mixture of four stereoisomers and only at these elevated concentrations did sfGFP production became significantly higher than background. Fidelity of each synthetase variant was determined by the amount of sfGFP produced from the same amber stop codon disrupted expression construct but in the absence of any ncAA in the media (indicated by the ‘-ncAA’ bar). For reference, native sfGFP without an amber stop codon expresses at ∼800-1000 mg per liter culture under identical conditions. Error bars represent the standard deviation of sfGFP produced from three independent expression cultures.
Ncaas, supplied by Rocha labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ncaas/pm27107466-24-8-16?v=Rocha+labs
Average 90 stars, based on 1 article reviews
ncaas - by Bioz Stars, 2026-08
90/100 stars
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ncaas  (Verlag GmbH)
90
Verlag GmbH ncaas
<t>Permissivity</t> profiles of the G2 and F9 synthetases. Reported is the amount of sfGFP with each of the <t>ncAAs</t> listed in Figure 1 incorporated at position 150. For each expression, ncAA concentration was 1 mM, except for Tco-amF (3) which was 5 mM because preparations of it consisted of an approximately equal mixture of four stereoisomers and only at these elevated concentrations did sfGFP production became significantly higher than background. Fidelity of each synthetase variant was determined by the amount of sfGFP produced from the same amber stop codon disrupted expression construct but in the absence of any ncAA in the media (indicated by the ‘-ncAA’ bar). For reference, native sfGFP without an amber stop codon expresses at ∼800-1000 mg per liter culture under identical conditions. Error bars represent the standard deviation of sfGFP produced from three independent expression cultures.
Ncaas, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ncaas/10__1002_slash_ange__201712536-41-24-7?v=Verlag+GmbH
Average 90 stars, based on 1 article reviews
ncaas - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


Permissivity profiles of the G2 and F9 synthetases. Reported is the amount of sfGFP with each of the ncAAs listed in Figure 1 incorporated at position 150. For each expression, ncAA concentration was 1 mM, except for Tco-amF (3) which was 5 mM because preparations of it consisted of an approximately equal mixture of four stereoisomers and only at these elevated concentrations did sfGFP production became significantly higher than background. Fidelity of each synthetase variant was determined by the amount of sfGFP produced from the same amber stop codon disrupted expression construct but in the absence of any ncAA in the media (indicated by the ‘-ncAA’ bar). For reference, native sfGFP without an amber stop codon expresses at ∼800-1000 mg per liter culture under identical conditions. Error bars represent the standard deviation of sfGFP produced from three independent expression cultures.

Journal: Chembiochem : a European journal of chemical biology

Article Title: Gleaning unexpected fruits from hard-won synthetases: Probing principles of permissivity in non-canonical amino acid-tRNA synthetases

doi: 10.1002/cbic.201402180

Figure Lengend Snippet: Permissivity profiles of the G2 and F9 synthetases. Reported is the amount of sfGFP with each of the ncAAs listed in Figure 1 incorporated at position 150. For each expression, ncAA concentration was 1 mM, except for Tco-amF (3) which was 5 mM because preparations of it consisted of an approximately equal mixture of four stereoisomers and only at these elevated concentrations did sfGFP production became significantly higher than background. Fidelity of each synthetase variant was determined by the amount of sfGFP produced from the same amber stop codon disrupted expression construct but in the absence of any ncAA in the media (indicated by the ‘-ncAA’ bar). For reference, native sfGFP without an amber stop codon expresses at ∼800-1000 mg per liter culture under identical conditions. Error bars represent the standard deviation of sfGFP produced from three independent expression cultures.

Article Snippet: Permissivity profile development for the G2 and F9 synthetases The ncAAs used in this study were purchased from Peptech (Burlington, MA), Bachem (Torrance, CA) or Sigma (St. Louis, MO), or was synthesized as previously described: tyrosine (Tyr, 1 ); 4-(2′-bromoisobutyramido)-phenylalanine, (BibaF 2 ) [ 15 ] ; 4-trans-cyclooctenamidopheylalanine, (Tco-amF, 3 ); acridon-2-ylalanine, (Acd, 4 ) [ 16 ] , 4-acetamidopheylalanine, (AmF, 5 ); 4-(6-methyl-s-tetrazine-3-yl) aminophenylalanine, (Tet-F, 6 ) [ 19 ] ; 4-aminophenylalanine, (AF, 7 ); 4-bromoisobutyryloxymethyl-L-phenylalanine (BiF, 8 ) [ 17 ] , 4-benzoyl-phenylalanine, (Bpa, 9 ); benzyltyrosine, (B-Tyr, 10 ), o-methyltyrosine, (M-Tyr, 11 ); thyronine, (Thy, 12 ); anthraquinonylalanine; (AQA, 13 ) [ 25 ] , 3-(3′-fluorenyl-9′-oxo)alanine, (FluA, 14 ) [ 26 ] ; 4-carbamoylphenylalanine (CarF, 15 ).

Techniques: Expressing, Concentration Assay, Variant Assay, Produced, Construct, Standard Deviation