nbp1 Search Results


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pla2g6  (Novus Biologicals)
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Novus Biologicals pla2g6
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rap1b  (Novus Biologicals)
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Novus Biologicals rap1b
Activation of the <t>Epac–Rap1b</t> signaling pathway by GIP alleviates brain damage. Relative mRNA levels of Epac (A), Rap1a (B), and Rap1b (C) in mock and GIP-overexpressing NB41A3 cells were determined using qPCR. (D) The protein expressions of Epac and Rap1b, as detected by western blotting. (E, F) Bright-field images and its quantification in the treatment of CE3F4, glutamate, and Ferrostain-1. (G, H) Mice sagittal sections from the cerebellum of 1-year-old mice were immunofluorescence stained for GIP (red), Epac1 (green), and DAPI (blue). (I) Coronal sections showing Epac and Rap1b staining, showing notable signal increases in the TG mice (scale bars represent 100 μm). Data are presented as means ± SEM from more than three independent experiments, and t-tests were performed to assess statistical significance: **P < 0.01.
Rap1b, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aco2  (Novus Biologicals)
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Molecular and biochemical characterization of GFAP-Coasy mice. (A) Relative Coasy mRNA expression at 15 days and 6 months in Ctrl (n = 4) and GFAP-Coasy (n = 4) mice brain, tested by qPCR. (B) Representative images of Western blot analysis and (C–J) densitometric quantification of (C) Coasy, (D) Dcx, (E) Gfap, (F) Ft-H, (G) Ft-L, (H) Dmt1, (I) TfR1, and (J) <t>Aco2</t> at 15 days and 6 months in Ctrl and GFAP-Coasy mice brain. (K) Specific activity of mitochondrial respiratory chain complex I, expressed as nmoles/min/mg of protein. * p < 0.05, ** p < 0.01; *** p < 0.001 (two-way ANOVA).
Aco2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rnr p53r2 rabbit polyclonal  (Novus Biologicals)
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Molecular and biochemical characterization of GFAP-Coasy mice. (A) Relative Coasy mRNA expression at 15 days and 6 months in Ctrl (n = 4) and GFAP-Coasy (n = 4) mice brain, tested by qPCR. (B) Representative images of Western blot analysis and (C–J) densitometric quantification of (C) Coasy, (D) Dcx, (E) Gfap, (F) Ft-H, (G) Ft-L, (H) Dmt1, (I) TfR1, and (J) <t>Aco2</t> at 15 days and 6 months in Ctrl and GFAP-Coasy mice brain. (K) Specific activity of mitochondrial respiratory chain complex I, expressed as nmoles/min/mg of protein. * p < 0.05, ** p < 0.01; *** p < 0.001 (two-way ANOVA).
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anti pnrc2  (Novus Biologicals)
92
Novus Biologicals anti pnrc2
Molecular and biochemical characterization of GFAP-Coasy mice. (A) Relative Coasy mRNA expression at 15 days and 6 months in Ctrl (n = 4) and GFAP-Coasy (n = 4) mice brain, tested by qPCR. (B) Representative images of Western blot analysis and (C–J) densitometric quantification of (C) Coasy, (D) Dcx, (E) Gfap, (F) Ft-H, (G) Ft-L, (H) Dmt1, (I) TfR1, and (J) <t>Aco2</t> at 15 days and 6 months in Ctrl and GFAP-Coasy mice brain. (K) Specific activity of mitochondrial respiratory chain complex I, expressed as nmoles/min/mg of protein. * p < 0.05, ** p < 0.01; *** p < 0.001 (two-way ANOVA).
Anti Pnrc2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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desmin novus biologicals nbp1 45143  (Novus Biologicals)
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Molecular and biochemical characterization of GFAP-Coasy mice. (A) Relative Coasy mRNA expression at 15 days and 6 months in Ctrl (n = 4) and GFAP-Coasy (n = 4) mice brain, tested by qPCR. (B) Representative images of Western blot analysis and (C–J) densitometric quantification of (C) Coasy, (D) Dcx, (E) Gfap, (F) Ft-H, (G) Ft-L, (H) Dmt1, (I) TfR1, and (J) <t>Aco2</t> at 15 days and 6 months in Ctrl and GFAP-Coasy mice brain. (K) Specific activity of mitochondrial respiratory chain complex I, expressed as nmoles/min/mg of protein. * p < 0.05, ** p < 0.01; *** p < 0.001 (two-way ANOVA).
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orai1  (Novus Biologicals)
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Novus Biologicals orai1
Molecular and biochemical characterization of GFAP-Coasy mice. (A) Relative Coasy mRNA expression at 15 days and 6 months in Ctrl (n = 4) and GFAP-Coasy (n = 4) mice brain, tested by qPCR. (B) Representative images of Western blot analysis and (C–J) densitometric quantification of (C) Coasy, (D) Dcx, (E) Gfap, (F) Ft-H, (G) Ft-L, (H) Dmt1, (I) TfR1, and (J) <t>Aco2</t> at 15 days and 6 months in Ctrl and GFAP-Coasy mice brain. (K) Specific activity of mitochondrial respiratory chain complex I, expressed as nmoles/min/mg of protein. * p < 0.05, ** p < 0.01; *** p < 0.001 (two-way ANOVA).
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icc if pit 1 novus biologicals nbp1 92273 ab 11030310  (Novus Biologicals)
93
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Molecular and biochemical characterization of GFAP-Coasy mice. (A) Relative Coasy mRNA expression at 15 days and 6 months in Ctrl (n = 4) and GFAP-Coasy (n = 4) mice brain, tested by qPCR. (B) Representative images of Western blot analysis and (C–J) densitometric quantification of (C) Coasy, (D) Dcx, (E) Gfap, (F) Ft-H, (G) Ft-L, (H) Dmt1, (I) TfR1, and (J) <t>Aco2</t> at 15 days and 6 months in Ctrl and GFAP-Coasy mice brain. (K) Specific activity of mitochondrial respiratory chain complex I, expressed as nmoles/min/mg of protein. * p < 0.05, ** p < 0.01; *** p < 0.001 (two-way ANOVA).
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Image Search Results


Activation of the Epac–Rap1b signaling pathway by GIP alleviates brain damage. Relative mRNA levels of Epac (A), Rap1a (B), and Rap1b (C) in mock and GIP-overexpressing NB41A3 cells were determined using qPCR. (D) The protein expressions of Epac and Rap1b, as detected by western blotting. (E, F) Bright-field images and its quantification in the treatment of CE3F4, glutamate, and Ferrostain-1. (G, H) Mice sagittal sections from the cerebellum of 1-year-old mice were immunofluorescence stained for GIP (red), Epac1 (green), and DAPI (blue). (I) Coronal sections showing Epac and Rap1b staining, showing notable signal increases in the TG mice (scale bars represent 100 μm). Data are presented as means ± SEM from more than three independent experiments, and t-tests were performed to assess statistical significance: **P < 0.01.

Journal: BMB Reports

Article Title: Glucose-dependent insulinotropic polypeptide (GIP) alleviates ferroptosis in aging-induced brain damage through the Epac/Rap1 signaling pathway

doi: 10.5483/BMBRep.2024-0067

Figure Lengend Snippet: Activation of the Epac–Rap1b signaling pathway by GIP alleviates brain damage. Relative mRNA levels of Epac (A), Rap1a (B), and Rap1b (C) in mock and GIP-overexpressing NB41A3 cells were determined using qPCR. (D) The protein expressions of Epac and Rap1b, as detected by western blotting. (E, F) Bright-field images and its quantification in the treatment of CE3F4, glutamate, and Ferrostain-1. (G, H) Mice sagittal sections from the cerebellum of 1-year-old mice were immunofluorescence stained for GIP (red), Epac1 (green), and DAPI (blue). (I) Coronal sections showing Epac and Rap1b staining, showing notable signal increases in the TG mice (scale bars represent 100 μm). Data are presented as means ± SEM from more than three independent experiments, and t-tests were performed to assess statistical significance: **P < 0.01.

Article Snippet: Western blotting was performed using antibodies against anti-GIP (GTX55639; GeneTex, Irvine, CA, USA), Epac (sc-28366, 1:1,000; Santa Cruz), RAP1B (NBP1-54871, 1:1,000; Novus Biologicals), xCT/SLC7A11 (MA5-35360, Thermo Fisher Scientific), Gpx-4 (sc-166570; Santa Cruz Biotechnology, Dallas, TX, USA), nuclear factor erythroid 2-related factor (Nrf2) (sc-365949), Nox1 (ab131088; Abcam), β-actin (sc-47778), and GAPDH (#5174; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Activation Assay, Western Blot, Immunofluorescence, Staining

Molecular and biochemical characterization of GFAP-Coasy mice. (A) Relative Coasy mRNA expression at 15 days and 6 months in Ctrl (n = 4) and GFAP-Coasy (n = 4) mice brain, tested by qPCR. (B) Representative images of Western blot analysis and (C–J) densitometric quantification of (C) Coasy, (D) Dcx, (E) Gfap, (F) Ft-H, (G) Ft-L, (H) Dmt1, (I) TfR1, and (J) Aco2 at 15 days and 6 months in Ctrl and GFAP-Coasy mice brain. (K) Specific activity of mitochondrial respiratory chain complex I, expressed as nmoles/min/mg of protein. * p < 0.05, ** p < 0.01; *** p < 0.001 (two-way ANOVA).

Journal: Frontiers in Cellular Neuroscience

Article Title: CoA synthase plays a critical role in neurodevelopment and neurodegeneration

doi: 10.3389/fncel.2024.1458475

Figure Lengend Snippet: Molecular and biochemical characterization of GFAP-Coasy mice. (A) Relative Coasy mRNA expression at 15 days and 6 months in Ctrl (n = 4) and GFAP-Coasy (n = 4) mice brain, tested by qPCR. (B) Representative images of Western blot analysis and (C–J) densitometric quantification of (C) Coasy, (D) Dcx, (E) Gfap, (F) Ft-H, (G) Ft-L, (H) Dmt1, (I) TfR1, and (J) Aco2 at 15 days and 6 months in Ctrl and GFAP-Coasy mice brain. (K) Specific activity of mitochondrial respiratory chain complex I, expressed as nmoles/min/mg of protein. * p < 0.05, ** p < 0.01; *** p < 0.001 (two-way ANOVA).

Article Snippet: A quantity of 10 to 35 μg of total proteins were separated using SDS-PAGE and then electroblotted onto a nitrocellulose membrane, which was subsequently incubated with specific antibodies for COASY (PA5-28696, Thermofisher Scientific, Waltham, MA, United States), DCX (#4604, Cell Signaling, Danvers, MA, United States), GFAP (MAB360, Millipore, Burlington, MA, United States), Ft-L and Ft-H , GAPDH (ab181602, Abcam, Cambridge, United Kingdom), TfR1 (13–6,800, Thermofisher Scientific, Waltham, MA, United States), DMT1 (sc-166884, Santa Cruz Biotechnology, Dallas, TX, United States), ACO2 (NBP1-32781, Novus Biologicals, Centennial, CO, United States), NCOA4 (sc-373739, Santa Cruz Biotechnology, Dallas, TX, United States).

Techniques: Expressing, Western Blot, Activity Assay