nb100-93329 Search Results


nup153  (Novus Biologicals)
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Novus Biologicals nup153
Fig. 1 | Biochemical characterization of CPSF6, NUP153and SEC24C interactions with HIV-1 cores and CA tubes. Representative immunoblots showing CA nanotube mediated co-pelleting of endogenous CPSF6 (a), <t>NUP153</t> (b) and SEC24C (c) from MT4 cell lysates. The proteins of interest were visualized by antibodies ab175237(Abcam) against CPSF6, NB100-93329 (Novus) against NUP153, ab122633 (Abcam) against SEC24C. Lane 1: cell lysate. Lane 2: supernatant or unbound fraction after pelleting in the absence of CA tubes. Lane 3: supernatant or unbound fraction after co-pelleting with pre-formed CA tubes. Lane 4: pelleted or bound fraction in the absence of CA tubes. Lane 5: pelleted or bound fraction in the presence of CA tubes. The experiments were repeated 3 times independently with similar results. Quantitation of GST-mediated affinity pull-down of native HIV-1 cores bound to indicated concentrations of GST-CPSF6261-358(LCR-FG-LCR) vs GST-CPSF6(FG)/nonLCR (d), GST-NUP1531306-1450(LCR-FG-LCR) vs GST-NUP153(FG)/nonLCR (e); and
Nup153, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nb100-93329/pm36202818-81-14-12?v=Novus+Biologicals
Average 93 stars, based on 1 article reviews
nup153 - by Bioz Stars, 2026-07
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Fig. 1 | Biochemical characterization of CPSF6, NUP153and SEC24C interactions with HIV-1 cores and CA tubes. Representative immunoblots showing CA nanotube mediated co-pelleting of endogenous CPSF6 (a), NUP153 (b) and SEC24C (c) from MT4 cell lysates. The proteins of interest were visualized by antibodies ab175237(Abcam) against CPSF6, NB100-93329 (Novus) against NUP153, ab122633 (Abcam) against SEC24C. Lane 1: cell lysate. Lane 2: supernatant or unbound fraction after pelleting in the absence of CA tubes. Lane 3: supernatant or unbound fraction after co-pelleting with pre-formed CA tubes. Lane 4: pelleted or bound fraction in the absence of CA tubes. Lane 5: pelleted or bound fraction in the presence of CA tubes. The experiments were repeated 3 times independently with similar results. Quantitation of GST-mediated affinity pull-down of native HIV-1 cores bound to indicated concentrations of GST-CPSF6261-358(LCR-FG-LCR) vs GST-CPSF6(FG)/nonLCR (d), GST-NUP1531306-1450(LCR-FG-LCR) vs GST-NUP153(FG)/nonLCR (e); and

Journal: Nature communications

Article Title: Prion-like low complexity regions enable avid virus-host interactions during HIV-1 infection.

doi: 10.1038/s41467-022-33662-6

Figure Lengend Snippet: Fig. 1 | Biochemical characterization of CPSF6, NUP153and SEC24C interactions with HIV-1 cores and CA tubes. Representative immunoblots showing CA nanotube mediated co-pelleting of endogenous CPSF6 (a), NUP153 (b) and SEC24C (c) from MT4 cell lysates. The proteins of interest were visualized by antibodies ab175237(Abcam) against CPSF6, NB100-93329 (Novus) against NUP153, ab122633 (Abcam) against SEC24C. Lane 1: cell lysate. Lane 2: supernatant or unbound fraction after pelleting in the absence of CA tubes. Lane 3: supernatant or unbound fraction after co-pelleting with pre-formed CA tubes. Lane 4: pelleted or bound fraction in the absence of CA tubes. Lane 5: pelleted or bound fraction in the presence of CA tubes. The experiments were repeated 3 times independently with similar results. Quantitation of GST-mediated affinity pull-down of native HIV-1 cores bound to indicated concentrations of GST-CPSF6261-358(LCR-FG-LCR) vs GST-CPSF6(FG)/nonLCR (d), GST-NUP1531306-1450(LCR-FG-LCR) vs GST-NUP153(FG)/nonLCR (e); and

Article Snippet: The proteins of interest were visualized by antibodies ab175237(Abcam) against CPSF6, NB100-93329 (Novus) against NUP153, ab122633 (Abcam) against SEC24C.

Techniques: Western Blot, Quantitation Assay