Journal: Scientific Reports

Article Title: Identification of a novel macrophage-related prognostic signature in colorectal cancer

doi: 10.1038/s41598-024-53207-9

Figure Lengend Snippet: Primer.

Article Snippet: Antibody against MMP3 was obtained from Bio-Techne.

Techniques:

Journal: Scientific Reports

Article Title: Identification of a novel macrophage-related prognostic signature in colorectal cancer

doi: 10.1038/s41598-024-53207-9

Figure Lengend Snippet: Univariate and multivariate cox regression.

Article Snippet: Antibody against MMP3 was obtained from Bio-Techne.

Techniques:

Journal: Scientific Reports

Article Title: Identification of a novel macrophage-related prognostic signature in colorectal cancer

doi: 10.1038/s41598-024-53207-9

Figure Lengend Snippet: The mRNA levels of hub genes. ( A ) The gene expression levels of hub genes in HCT116 and FHC cell. The FHC cells are normal human intestinal epithelial cells, while HCT116 cells are human colorectal carcinoma cells. ( B ) The gene expression levels of hub genes in CRC tissue and Noncancerous tissue from human patients. ( C ) Representative images and statistics of IHC staining for MMP3, C5AR1, TIMP1, ADAM8 in colon tissues and CRC from the Human Protein Atlas dataset. ( D ) The protein expression levels of MMP3 ("F" represents FHC cells, and "H" represents HCT116 cells), ADAM8, TIMP1, and C5AR1 were assessed in FHC cells and HCT116 cells, with GAPDH serving as a reference. Statistical significance levels were denoted as *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

Article Snippet: Antibody against MMP3 was obtained from Bio-Techne.

Techniques: Expressing, Immunohistochemistry

Journal: Scientific Reports

Article Title: Identification of a novel macrophage-related prognostic signature in colorectal cancer

doi: 10.1038/s41598-024-53207-9

Figure Lengend Snippet: Illustration of the roles of SPP1, C5AR1, MMP3, TIMP1, and ADAM8 in the progression of colorectal cancer. Created by biorender.

Article Snippet: Antibody against MMP3 was obtained from Bio-Techne.

Techniques:

Journal: Annals of Translational Medicine

Article Title: MicroRNA-25-3p therapy for intervertebral disc degeneration by targeting the IL-1β/ZIP8/MTF1 signaling pathway with a novel thermo-responsive vector

doi: 10.21037/atm-20-6595

Figure Lengend Snippet: The influence of IL-1β on ECM-degrading enzyme (MMP3, MMP13 and ADAMTS5) and ECM protein (collagen type II and aggrecan) expression. (A) Relative expression levels of MMP3 mRNA, MMP13 mRNA and ADAMTS5 mRNA in IL-1β-stimulated nucleus pulposus (NP) cells (10 ng/mL) and control cells via qRT-PCR. (B) Relative protein expression levels of MMP3, MMP13 and ADAMTS5 were examined in IL-1β-stimulated NP cells (10 ng/mL) and control cells via western blotting. (C) Relative protein expression levels of collagen type II and aggrecan were detected in IL-1β-stimulated NP cells (10 ng/mL) and control cells via immunocytochemistry (ICC). The bar is 50 µm. Data are the mean ± standard deviation (SD) of triplicate results. *, P<0.05.

Article Snippet: Membranes were incubated overnight at 4 °C with anti-IL-1β antibody (12703, 1:1,000, CST, USA), anti-MTF1 antibody (ab184119, 1:1,000, Abcam, Cambridge, UK), anti-ZIP8 antibody (NBP1-76505, 1:2,000, Novus, Biologicals, Colorado, USA), anti-MMP3 antibody (NB100-91878, 1:1,000, Novus Biologicals, Colorado, USA), anti-MMP13 antibody (NBP2-45887, 1:1,000, Novus, Biologicals, Colorado, USA), anti-ADAMTS5 antibody (NBP2-15286, 1:1,000, Novus, Biologicals, Colorado,USA), anti-β-actin antibody (abs118937, 1:3,000, Absin, Shanghai, China), and anti-PCNA antibody (abs13075, 1:2,000, Absin, Shanghai, China).

Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot, Immunocytochemistry, Standard Deviation

Journal: Annals of Translational Medicine

Article Title: MicroRNA-25-3p therapy for intervertebral disc degeneration by targeting the IL-1β/ZIP8/MTF1 signaling pathway with a novel thermo-responsive vector

doi: 10.21037/atm-20-6595

Figure Lengend Snippet: The effects of miRNA-25-3p modulation on IL-1β-stimulated ECM-degrading enzyme (MMP3, MMP13 and ADAMTS5) and ECM protein (collagen type II and aggrecan) expression. (A) Relative mRNA expression levels of MMP3, MMP13 and ADAMTS5 in the miRNA-25-3p mimic group and the control group determined via qRT-PCR. (B) Relative protein expression levels of MMP3, MMP13 and ADAMTS5 were examined in the miRNA-25-3p mimic group and the control group via western blotting. (C) Relative protein expression levels of collagen type II and aggrecan were detected in the miRNA-25-3p mimic group and the control group via immunocytochemistry (ICC). The bar is 50 µm. Data are the mean ± standard deviation (SD) of triplicate results. *, P<0.05.

Article Snippet: Membranes were incubated overnight at 4 °C with anti-IL-1β antibody (12703, 1:1,000, CST, USA), anti-MTF1 antibody (ab184119, 1:1,000, Abcam, Cambridge, UK), anti-ZIP8 antibody (NBP1-76505, 1:2,000, Novus, Biologicals, Colorado, USA), anti-MMP3 antibody (NB100-91878, 1:1,000, Novus Biologicals, Colorado, USA), anti-MMP13 antibody (NBP2-45887, 1:1,000, Novus, Biologicals, Colorado, USA), anti-ADAMTS5 antibody (NBP2-15286, 1:1,000, Novus, Biologicals, Colorado,USA), anti-β-actin antibody (abs118937, 1:3,000, Absin, Shanghai, China), and anti-PCNA antibody (abs13075, 1:2,000, Absin, Shanghai, China).

Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot, Immunocytochemistry, Standard Deviation