nb100-81927 Search Results


anti foxo1 fkhr ser256 nb100 81927  (Novus Biologicals)
92
Novus Biologicals anti foxo1 fkhr ser256 nb100 81927
Anti Foxo1 Fkhr Ser256 Nb100 81927, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nb100-81927/FoxO1%2FFKHR+%5Bp+Ser256%5D+Antibody/pm38322117-119-22-27
Average 92 stars, based on 1 article reviews
anti foxo1 fkhr ser256 nb100 81927 - by Bioz Stars, 2026-09
92/100 stars
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p foxo1  (Novus Biologicals)
94
Novus Biologicals p foxo1
Fig. 5 <t>FOXO1</t> mediated the protective effect of FKBP5 inhibition on β cells. A, B Western blot analysis of Pdx1, p-Foxo1Ser256, Foxo1, p-Akt Ser473 and Akt in NIT-1 cells transfected with siFoxo1 or control siRNAs, and then treated with SAFit2 (A). Relative proteins expression levels were quantified by Image J (B). C, D Annexin-V/7-AAD staining and flow cytometry analysis in NIT-1 cells transfected with siFoxo1 or control siRNAs and then treated with cytokines, or cytokines + SAFit2 (C). Quantification of PE+7AAD+/PE+7AAD- cells (D). E, F Western blot analysis of FOXO1, BCL2, BAX in primary human islets transfected with siFOXO1 or control siRNAs and then treated with cytokines, or cytokines + SAFit2 (E). Relative proteins expression levels were quantified by Image J (F). Experiments were repeated for 3 times. Student’s t-test. Mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.
P Foxo1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nb100-81927/FoxO1%2FFKHR+%5Bp+Ser256%5D+Antibody/pm37452039-220-26-29
Average 94 stars, based on 1 article reviews
p foxo1 - by Bioz Stars, 2026-09
94/100 stars
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Image Search Results


Fig. 5 FOXO1 mediated the protective effect of FKBP5 inhibition on β cells. A, B Western blot analysis of Pdx1, p-Foxo1Ser256, Foxo1, p-Akt Ser473 and Akt in NIT-1 cells transfected with siFoxo1 or control siRNAs, and then treated with SAFit2 (A). Relative proteins expression levels were quantified by Image J (B). C, D Annexin-V/7-AAD staining and flow cytometry analysis in NIT-1 cells transfected with siFoxo1 or control siRNAs and then treated with cytokines, or cytokines + SAFit2 (C). Quantification of PE+7AAD+/PE+7AAD- cells (D). E, F Western blot analysis of FOXO1, BCL2, BAX in primary human islets transfected with siFOXO1 or control siRNAs and then treated with cytokines, or cytokines + SAFit2 (E). Relative proteins expression levels were quantified by Image J (F). Experiments were repeated for 3 times. Student’s t-test. Mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

Journal: Cell death discovery

Article Title: The inhibition of FKBP5 protects β-cell survival under inflammation stress via AKT/FOXO1 signaling.

doi: 10.1038/s41420-023-01506-x

Figure Lengend Snippet: Fig. 5 FOXO1 mediated the protective effect of FKBP5 inhibition on β cells. A, B Western blot analysis of Pdx1, p-Foxo1Ser256, Foxo1, p-Akt Ser473 and Akt in NIT-1 cells transfected with siFoxo1 or control siRNAs, and then treated with SAFit2 (A). Relative proteins expression levels were quantified by Image J (B). C, D Annexin-V/7-AAD staining and flow cytometry analysis in NIT-1 cells transfected with siFoxo1 or control siRNAs and then treated with cytokines, or cytokines + SAFit2 (C). Quantification of PE+7AAD+/PE+7AAD- cells (D). E, F Western blot analysis of FOXO1, BCL2, BAX in primary human islets transfected with siFOXO1 or control siRNAs and then treated with cytokines, or cytokines + SAFit2 (E). Relative proteins expression levels were quantified by Image J (F). Experiments were repeated for 3 times. Student’s t-test. Mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: The blotted membranes were blocked with 5% skim milk for 60min at room temperature and incubated with primary antibodies FKBP51 (1:1000, ABclonal, A3863, Woburn, MA, USA), p-FOXO1 (1:1000, NB100-81927, Novus Biological, Cambridge, UK), FOXO1 (1:1000, CST-2880, Cell signaling technology, Danvers, MA, USA), p-AKT(1:1000, CST4060S, Cell signaling technology, Danvers, MA, USA), AKT(1:1000, CST9272S, Cell signaling technology, Danvers, MA, USA), PDX1(1:1000, CST9679S, Cell signaling technology, Danvers, MA, USA), Sqstm1/p62 (1:1000, ab56416, Abcam, Cambridge, MA, USA), LC3I/II (1:1000, CST-12741S, Cell signaling technology, Danvers, MA, USA), BAX(1:1000, ab32503, Abcam, Cambridge, MA, USA), BCL2(1:1000, ab59348, Cambridge, MA, USA), β-actin (1:1000, CST-3700, Cell signaling technology, Danvers, MA, USA), Histone cluster 1, H3a (1:1000, 17168-1-AP, Proteintech, Rosemont, IL 60018, USA) at 4°C overnight.

Techniques: Inhibition, Western Blot, Transfection, Control, Expressing, Staining, Cytometry