nb100-65411pe Search Results


rat anti hla abc pe  (Novus Biologicals)
90
Novus Biologicals rat anti hla abc pe
Rat Anti Hla Abc Pe, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nb100-65411pe/HLA+ABC+Antibody+(YTH862%2E2)+%5BPE%5D/pmc07138101-483-21-25
Average 90 stars, based on 1 article reviews
rat anti hla abc pe - by Bioz Stars, 2026-09
90/100 stars
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mhc  (Bio-Techne corporation)
91
Bio-Techne corporation mhc
Neuroblastoma <t>tumor</t> <t>organoids</t> are killed by TEG002 in the presence of Pamidronate, independent of <t>MHC-I</t> expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.
Mhc, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nb100-65411pe/HLA+ABC+Antibody/pmc08471928-54-6-24
Average 91 stars, based on 1 article reviews
mhc - by Bioz Stars, 2026-09
91/100 stars
  Buy from Supplier

Image Search Results


Neuroblastoma tumor organoids are killed by TEG002 in the presence of Pamidronate, independent of MHC-I expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.

Journal: Journal of Personalized Medicine

Article Title: αβ-T Cells Engineered to Express γδ-T Cell Receptors Can Kill Neuroblastoma Organoids Independent of MHC-I Expression

doi: 10.3390/jpm11090923

Figure Lengend Snippet: Neuroblastoma tumor organoids are killed by TEG002 in the presence of Pamidronate, independent of MHC-I expression. ( A ) Expression of HLA-a,b,c of all six organoids, measured by flow cytometry. Green indicates “No Activation” and red indicates “Activation” of TEG002. ( B ) Detailed dynamics of AMC691B killing by TEG002 over time. Representative graphs of one effector donor, which was tested in three different E:T ratios, are shown. Red line indicates the maximal cell lysis induced by the lysis control. ( C ) Killing of all four organoids by TEG002 over time at a 1:1 E:T ratio. AMC691T, AMC691B, and NB067 were tested with effectors from two different donors and NB129 with effectors from one donor. Graphs show mean of all experiments. ( D ) Bargraph of cytolysis at 48 h. Only the 1:1 E:T ratio is shown here. ( E ) Expression of HLA-a,b,c on four selected organoids in co-culture setting, measured by flow cytometry. Median Fluorescence Intensity (MFI) of HLA-a,b,c was used. Flow cytometry was performed only on organoids in co-culture with TEG002, with the addition of PAM at E:T 1:1. A Friedman test and a Dunn’s post hoc test, in comparison to T = 0, were performed to determine significant upregulation. ( F ) Cytolysis of AMC691T and AMC691B by TEG002 and untransduced αβ-T cells in the presence of 10μg/mL MHC-I blocking antibody (clone W6/32, Bio-Techne, ) or isotype control. Lysis control = Puromycin (1 μg/mL); PAM = Pamidronate; E:T = Effector to Target; all data are normalized to the target only condition; lysis control at 24 h.

Article Snippet: For two organoids, the cytotoxicity and MHC-I independent recognition was tested in the presence of 10 μg/mL MHC-I blocking antibody (clone W6.32, Cat. NB100-64775, BioTechne, Minneapolis, MN, USA) or 10 μg/mL IgG isotype control (Cat. 31903, ThermoFisher, Waltham, MA, USA).

Techniques: Expressing, Flow Cytometry, Activation Assay, Lysis, Co-Culture Assay, Fluorescence, Comparison, Blocking Assay