nb100-64650 Search Results


somatostatin antibody (yc7)  (Bio-Techne corporation)
92
Bio-Techne corporation somatostatin antibody (yc7)
Somatostatin Antibody (Yc7), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/somatostatin antibody (yc7)/product/Bio-Techne corporation
Average 92 stars, based on 1 article reviews
somatostatin antibody (yc7) - by Bioz Stars, 2026-06
92/100 stars
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somatostatin  (Novus Biologicals)
93
Novus Biologicals somatostatin
Fig. 5 PC1/3 expression in alpha cells. (a) Representa- tive images showing PC1/3, glucagon and <t>somatostatin</t> expression in islets from donors with or without diabetes. nPOD case numbers are indicated in the merged images. Scale bar, 100 μm. (b) Proportional glucagon-positive area in islets. (c) Median RFU of glucagon in islets. (d–f) Percentage of glucagon-positive, PC1/3- positive (d), glucagon-positive, PC1/3-negative (e) and gluca- gon-positive islet cells (f) in islets. In (f) the darker columns represent PC1/3-negative cells, the lighter columns represent PC1/3-positive cells. (g) Mean RFU of glucagon in glucagon- positive alpha cells. (h) Mean RFU of glucagon in PC1/3-pos- itive vs PC1/3-negative cells. (i) Median RFU of PC1/3 in PC1/3-positive, glucagon-posi- tive alpha cells. (j) Mean PC1/3 and glucagon co-localisation in islets. Data are shown for islets from non-diabetic donors (filled circles, older adults; empty circles, young adults), AAb+ donors (filled circles, double AAb+; empty circles, single AAb+), donors with recent- onset type 1 diabetes (dura- tion <5 years), donors with long-duration type 1 diabetes (>15 years) and donors with type 2 diabetes. Each data point represents the average value from one donor. Hatched bars show data for images analysed at the islet level; non-hatched bars show data for images analysed at the single-cell level. Normally distributed data (b, g, h, j) are expressed as mean ± SEM, non-normal data (c, d, e, f, i) are expressed as median ± IQR. *p<0.05, **p<0.01 and ***p<0.001. GCG, glucagon; ND, non-diabetic; T1D, type 1 diabetes; T2D, type 2 diabetes; y, years
Somatostatin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/somatostatin/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
somatostatin - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier

Image Search Results


Fig. 5 PC1/3 expression in alpha cells. (a) Representa- tive images showing PC1/3, glucagon and somatostatin expression in islets from donors with or without diabetes. nPOD case numbers are indicated in the merged images. Scale bar, 100 μm. (b) Proportional glucagon-positive area in islets. (c) Median RFU of glucagon in islets. (d–f) Percentage of glucagon-positive, PC1/3- positive (d), glucagon-positive, PC1/3-negative (e) and gluca- gon-positive islet cells (f) in islets. In (f) the darker columns represent PC1/3-negative cells, the lighter columns represent PC1/3-positive cells. (g) Mean RFU of glucagon in glucagon- positive alpha cells. (h) Mean RFU of glucagon in PC1/3-pos- itive vs PC1/3-negative cells. (i) Median RFU of PC1/3 in PC1/3-positive, glucagon-posi- tive alpha cells. (j) Mean PC1/3 and glucagon co-localisation in islets. Data are shown for islets from non-diabetic donors (filled circles, older adults; empty circles, young adults), AAb+ donors (filled circles, double AAb+; empty circles, single AAb+), donors with recent- onset type 1 diabetes (dura- tion <5 years), donors with long-duration type 1 diabetes (>15 years) and donors with type 2 diabetes. Each data point represents the average value from one donor. Hatched bars show data for images analysed at the islet level; non-hatched bars show data for images analysed at the single-cell level. Normally distributed data (b, g, h, j) are expressed as mean ± SEM, non-normal data (c, d, e, f, i) are expressed as median ± IQR. *p<0.05, **p<0.01 and ***p<0.001. GCG, glucagon; ND, non-diabetic; T1D, type 1 diabetes; T2D, type 2 diabetes; y, years

Journal: Diabetologia

Article Title: Quantitative analysis of islet prohormone convertase 1/3 expression in human pancreas donors with diabetes.

doi: 10.1007/s00125-024-06275-5

Figure Lengend Snippet: Fig. 5 PC1/3 expression in alpha cells. (a) Representa- tive images showing PC1/3, glucagon and somatostatin expression in islets from donors with or without diabetes. nPOD case numbers are indicated in the merged images. Scale bar, 100 μm. (b) Proportional glucagon-positive area in islets. (c) Median RFU of glucagon in islets. (d–f) Percentage of glucagon-positive, PC1/3- positive (d), glucagon-positive, PC1/3-negative (e) and gluca- gon-positive islet cells (f) in islets. In (f) the darker columns represent PC1/3-negative cells, the lighter columns represent PC1/3-positive cells. (g) Mean RFU of glucagon in glucagon- positive alpha cells. (h) Mean RFU of glucagon in PC1/3-pos- itive vs PC1/3-negative cells. (i) Median RFU of PC1/3 in PC1/3-positive, glucagon-posi- tive alpha cells. (j) Mean PC1/3 and glucagon co-localisation in islets. Data are shown for islets from non-diabetic donors (filled circles, older adults; empty circles, young adults), AAb+ donors (filled circles, double AAb+; empty circles, single AAb+), donors with recent- onset type 1 diabetes (dura- tion <5 years), donors with long-duration type 1 diabetes (>15 years) and donors with type 2 diabetes. Each data point represents the average value from one donor. Hatched bars show data for images analysed at the islet level; non-hatched bars show data for images analysed at the single-cell level. Normally distributed data (b, g, h, j) are expressed as mean ± SEM, non-normal data (c, d, e, f, i) are expressed as median ± IQR. *p<0.05, **p<0.01 and ***p<0.001. GCG, glucagon; ND, non-diabetic; T1D, type 1 diabetes; T2D, type 2 diabetes; y, years

Article Snippet: A previously reported protocol [20] was used, with the following primary antibody cocktails reconstituted in an antibody diluent (S080983-2; Agilent Technologies, CA, USA): (1) insulin (IR002, Agilent Technologies, RRID:AB_2800361, pan-insulin antibody, 1:4 dilution), proinsulin (GS-9A8, Developmental Studies Hybridoma Bank, IA, USA, RRID:AB_532383, detects the B-C junction of proinsulin, 1:50 dilution), PC1/3 (ab191452, Abcam, Cambridge, UK, RRID:AB_3064864, 1:500 dilution, likely detects both 87 kDa and 66 kDa forms of PC1/3); and (2) glucagon (ab92517, Abcam, RRID:AB_10561971, 1:1000 dilution), somatostatin (NB100–64650, Novus Biologicals, CO, USA, RRID:AB_965456, 1:200 dilution) and PC1/3 (ab191452, Abcam, RRID:AB_3064864, 1:500 dilution).

Techniques: Expressing