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antibodies against ilk  (Novus Biologicals)
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Novus Biologicals antibodies against ilk
Fig. 1. <t>ILK</t> expression and inactivation in Osxþ osteoprogenitors. (A) Relative mRNA expression levels as determined by qRT-PCR of the osteoblast marker genes Osx,Opn,andOcn(leftgraph),andoftheintegrinsignalingcomponentsItgb1,FAK,andILK(rightgraph),inFACS-sortedcellpopulationsderivedfromlongbones of newborn <t>mice.</t> <t>Osx-Cre:GFP–</t> and Osx-Cre:GFPþ cell fractions were collected from Osx-Cre:GFP-expressing pups (n¼ 5), and a parallel “full digest” control sample was harvested from Osx-Cre:GFP-negative littermates (n¼ 3). Bars represent the mean SE. Significant differences are indicated as p< 0.05 and p< 0.01, by one-way ANOVA followed by Tukey multiple comparison test (Itgb1 and FAK), or Kruskal-Wallis test (Osx, Opn, Ocn, ILK). (B) Relative ILK mRNA expression level in Osx-Cre:GFPþ cells isolated by FACS from long bones of newborn control and ILK cKO pups (including per genotype n¼ 3 independent cell pools isolated from 4 to 6 pups each). Bars represent the mean SE. p< 0.01, Student’s t test. (C, D) Immunofluorescent staining for ILK (red detection) and GFP (green) in tibias of (C) embryonic (E16.5) and (D) adult (12-week-old) control and ILK cKO mice. Nuclei are stained by Hoechst (blue). Yellow arrowheads point at Osx-Cre:GFPþ cells,whicharemostlyGFPþ/ILKþ doublepositivecellsincontrolsectionsbutlacktheILKsignalinILKcKOmice;whitearrowheadsindicateOsx-Cre: GFP-negative cells expressing ILK in both genotypes. POC¼ primary ossification center; tb¼ trabecular bone; GP¼ growth plate. Scale bars¼ 50mm.
Antibodies Against Ilk, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 1. ILK expression and inactivation in Osxþ osteoprogenitors. (A) Relative mRNA expression levels as determined by qRT-PCR of the osteoblast marker genes Osx,Opn,andOcn(leftgraph),andoftheintegrinsignalingcomponentsItgb1,FAK,andILK(rightgraph),inFACS-sortedcellpopulationsderivedfromlongbones of newborn mice. Osx-Cre:GFP– and Osx-Cre:GFPþ cell fractions were collected from Osx-Cre:GFP-expressing pups (n¼ 5), and a parallel “full digest” control sample was harvested from Osx-Cre:GFP-negative littermates (n¼ 3). Bars represent the mean SE. Significant differences are indicated as p< 0.05 and p< 0.01, by one-way ANOVA followed by Tukey multiple comparison test (Itgb1 and FAK), or Kruskal-Wallis test (Osx, Opn, Ocn, ILK). (B) Relative ILK mRNA expression level in Osx-Cre:GFPþ cells isolated by FACS from long bones of newborn control and ILK cKO pups (including per genotype n¼ 3 independent cell pools isolated from 4 to 6 pups each). Bars represent the mean SE. p< 0.01, Student’s t test. (C, D) Immunofluorescent staining for ILK (red detection) and GFP (green) in tibias of (C) embryonic (E16.5) and (D) adult (12-week-old) control and ILK cKO mice. Nuclei are stained by Hoechst (blue). Yellow arrowheads point at Osx-Cre:GFPþ cells,whicharemostlyGFPþ/ILKþ doublepositivecellsincontrolsectionsbutlacktheILKsignalinILKcKOmice;whitearrowheadsindicateOsx-Cre: GFP-negative cells expressing ILK in both genotypes. POC¼ primary ossification center; tb¼ trabecular bone; GP¼ growth plate. Scale bars¼ 50mm.

Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Article Title: Cross-sectional analysis of the association between fragility fractures and bone microarchitecture in older men: the STRAMBO study.

doi: 10.1002/jbmr.319

Figure Lengend Snippet: Fig. 1. ILK expression and inactivation in Osxþ osteoprogenitors. (A) Relative mRNA expression levels as determined by qRT-PCR of the osteoblast marker genes Osx,Opn,andOcn(leftgraph),andoftheintegrinsignalingcomponentsItgb1,FAK,andILK(rightgraph),inFACS-sortedcellpopulationsderivedfromlongbones of newborn mice. Osx-Cre:GFP– and Osx-Cre:GFPþ cell fractions were collected from Osx-Cre:GFP-expressing pups (n¼ 5), and a parallel “full digest” control sample was harvested from Osx-Cre:GFP-negative littermates (n¼ 3). Bars represent the mean SE. Significant differences are indicated as p< 0.05 and p< 0.01, by one-way ANOVA followed by Tukey multiple comparison test (Itgb1 and FAK), or Kruskal-Wallis test (Osx, Opn, Ocn, ILK). (B) Relative ILK mRNA expression level in Osx-Cre:GFPþ cells isolated by FACS from long bones of newborn control and ILK cKO pups (including per genotype n¼ 3 independent cell pools isolated from 4 to 6 pups each). Bars represent the mean SE. p< 0.01, Student’s t test. (C, D) Immunofluorescent staining for ILK (red detection) and GFP (green) in tibias of (C) embryonic (E16.5) and (D) adult (12-week-old) control and ILK cKO mice. Nuclei are stained by Hoechst (blue). Yellow arrowheads point at Osx-Cre:GFPþ cells,whicharemostlyGFPþ/ILKþ doublepositivecellsincontrolsectionsbutlacktheILKsignalinILKcKOmice;whitearrowheadsindicateOsx-Cre: GFP-negative cells expressing ILK in both genotypes. POC¼ primary ossification center; tb¼ trabecular bone; GP¼ growth plate. Scale bars¼ 50mm.

Article Snippet: For simultaneous ILK and GFP immunostaining, the sections were first blockedwith5%normalgoat serumand then incubatedovernight at 4°Cwith antibodies against ILK (Novus Biologicals, Littleton, CO, USA; NB100-60451, at 2mg/ml) and GFP (Abcam, Cambridge, UK; ab13970; at 10mg/ml), followedby anAlexa Fluor 546-conjugated goat-anti-rabbit antibody (Thermo Fisher Scientific, Waltham, CA, USA; at 5mg/mL) and goat-anti-chicken IgY H&L DyLight 488 (Abcam; ab96951, at 5mg/mL), for 2 hours.

Techniques: Expressing, Quantitative RT-PCR, Marker, Control, Comparison, Isolation, Staining