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Novus Biologicals
setx  Setx, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/nb100-57542/pmc03814643-113-8-22?v=Novus+Biologicals Average 94 stars, based on 1 article reviews
setx - by Bioz Stars,
2026-08
94/100 stars
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Buy from Supplier |
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Novus Biologicals
anti senataxin Anti Senataxin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/nb100-57542/pmc11999364-58-45-46?v=Novus+Biologicals Average 94 stars, based on 1 article reviews
anti senataxin - by Bioz Stars,
2026-08
94/100 stars
|
Buy from Supplier |
Image Search Results
Journal: Genes & Development
Article Title: A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage
doi: 10.1101/gad.224923.113
Figure Lengend Snippet: A SETX–Rrp45 interaction detected in yeast is blocked by AOA2 mutations and mediated by Rrp45 SIM. ( A ) Drop test assay of the mated yeast strains CG1945 carrying pAS2ΔΔ-Nter-SETX and Y187 carrying the empty pACTII vector or expressing Rrp45-Cter (pACTII-Rrp45-Cter). Days of growth are indicated at the bottom . ( B ) Schematic representation of SETX. ( C ) Drop test assay as in A of HF7c yeast strain carrying pACTII-Rrp45-Cter and empty pAS2ΔΔ (−) or expressing Nter-SETX wild type (WT), an AOA2 mutation (EK, WC, or PL), or an ALS4 mutation (TI or LS). Days of growth are indicated at the right . ( D ) Schematic representation of Rrp45 FL and del1. ( E ) Drop test assay as in C . HF7c carrying empty pAS2ΔΔ or expressing Nter-SETX wild type (pAS2ΔΔ-Nter-SETX WT) and pACTII-Rrp45 FL with (FL) or without (FL-ΔSIM) SIM and pACTII-del1 with (del1) or without (del1-ΔSIM) SIM. ( F ) Drop test assay as in C , with pAS2ΔΔ-Nter-SETX wild type and pACTII carrying del1 wild type or mutants of the SIM domain as indicated.
Article Snippet: Abs used for immunoprecipitations and/or Western blot were
Techniques: Plasmid Preparation, Expressing, Mutagenesis
Journal: Genes & Development
Article Title: A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage
doi: 10.1101/gad.224923.113
Figure Lengend Snippet: Nter-SETX is sumoylated in yeast and in HeLa cells by SUMO2/3. ( A ) Gal4DBD immunoprecipitation from extracts of HF7c cells expressing HA-Rrp45-Cter and Gal4DBD (lane a ) or Gal4DBD-Nter-SETX wild type (lane b ). Nter-SETX expressed in yeast was detected by Western blot using an anti-Gal4DBD, and sumoylated Nter-SETX was detected using an anti-Smt3 antibody (Ab). ( B ) Flag immunoprecipitation of Flag-tagged Nter-SETX expressed in HeLa cells. Nter-SETX isoforms were detected by Western blot using anti-Flag M2 Ab. ( C ) Flag immunoprecipitation of Flag-tagged Nter-SETX in HeLa cells stably expressing 6xHis-SUMO1 (lane 1 ), 6xHis-SUMO2 (lane 2 ), and 6xHis-SUMO3 (lane 3 ). Nter-SETX isoforms were detected by Western blot using an M2-Flag Ab, and sumoylated Nter-SETX was detected with an anti-His Ab. (IB) Immunoblot; (I) input; (IP) immunoprecipitate.
Article Snippet: Abs used for immunoprecipitations and/or Western blot were
Techniques: Immunoprecipitation, Expressing, Western Blot, Stable Transfection
Journal: Genes & Development
Article Title: A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage
doi: 10.1101/gad.224923.113
Figure Lengend Snippet: AOA2 mutations block Nter-SETX sumoylation. ( A ) Flag immunoprecipitation in 6xHis-SUMO3 HeLa cells transfected with empty vector (−) and Nter-SETX wild type, PL, and LS and cotransfected with Nter–SETX wild type (WT) and Myc-Ubc9 (WT+Ubc9). ( B ) Flag immunoprecipitation in 6xHis-SUMO3 HeLa cells transfected with Myc-Ubc9 and empty vector (−), Nter-SETX wild type, Nter-SETX carrying an AOA2 mutation (PL, EK, or WC), and the LS ALS4 mutation. ( C ) Flag immunoprecipitation as in B , with 6xHis-SUMO3 HeLa cells transfected with Myc-Ubc9 and empty vector (−), Nter-SETX wild type, the two ALS4 mutants (LS and TI), and the PL and EK AOA2 mutants. Nter-SETX was detected by Western blot using an anti-Flag Ab, sumoylated SETX was detected with an anti-His Ab, and Myc-Ubc9 and endogenous Ubc9 were detected with an anti-Ubc9 Ab. ( D ) Ni ++ bead purification in denaturing condition of Nter-SETX wild type, TI, and EK cotransfected in 6xHis-SUMO3 HeLa cells with Myc-Ubc9. SETX isoforms were detected by Western blot using anti-Flag Ab. ( E ) Drop test assay as in . HF7c yeast strain expressed HA-Ubc9 and Gal4DBD-Nter-SETX wild type and mutants. Cells grew for 3 d. (IB) Immunoblot; (I) input; (IP) immunoprecipitation.
Article Snippet: Abs used for immunoprecipitations and/or Western blot were
Techniques: Blocking Assay, Immunoprecipitation, Transfection, Plasmid Preparation, Mutagenesis, Western Blot, Purification
Journal: Genes & Development
Article Title: A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage
doi: 10.1101/gad.224923.113
Figure Lengend Snippet: SETX and Rrp45 associate and colocalize in stress-induced nuclear foci. ( A ) Western blots of SETX and Rrp45 immunoprecipitations from HeLa nuclear extract. Blots were probed with anti-SETX and anti-Rrp45 ( left panel) or anti-SUMO2 ( right panel) Abs. Arrows indicate unmodified SETX and Rrp45. ( B ) Western blot analysis ( left panel) and quantification ( right panel) of U87 whole-cell extracts after Rrp6 and Rrp45 knockdown. Proteins levels were normalized to GAPDH ( n = 5; mean and SE are shown). ( C ) Immunofluorescence imaging of SETX and Rrp45 in HeLa cells after aphidicolin (APH) or control (DMSO) treatment for 24 h. Colocalizing foci in a typical cell (Merge) are highlighted with arrows. Bar, 10 μm. ( D ) Quantification of SETX foci colocalizing with Rrp45 foci after DMSO and APH treatment ( n = 50 cells). Similar results were observed in three independent experiments. ( E ) Three-dimensional immunofluorescence imaging of SETX and transient expression of RNase H1-mCherry ( top panel) and Rrp45 and transient expression of GFP-RNase H1 ( bottom panel) in HeLa cells. A total of 30 GFP-RNase H1-expressing cells were inspected, and none showed Rrp45 foci. Bar, 10 μm.
Article Snippet: Abs used for immunoprecipitations and/or Western blot were
Techniques: Western Blot, Knockdown, Immunofluorescence, Imaging, Control, Expressing