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Journal: International Journal of Molecular Sciences
Article Title: MiR-7-5p Is Involved in Ferroptosis Signaling and Radioresistance Thru the Generation of ROS in Radioresistant HeLa and SAS Cell Lines
doi: 10.3390/ijms22158300
Figure Lengend Snippet: Expression of ALOXs in CRR cells. ALOX5 , 12, and 15 expressions in parent and CRR cells were detected using qPCR ( A – C ) and western blotting ( D ). A: Gene expression of ALOX5 . ( B ) Gene expression of ALOX12 . C: Gene expression of ALOX15 . **: p < 0.01 using Student’s t -test. Each qPCR was performed 3 times. Gene expressions of ALOX5 , 12 , and 15 were all significantly down-regulated in CRR cells. ( D ) Western blotting of ALOX5 , 12 , and 15 . The table shows the expression level of each ALOX s in CRR cells. After correcting the intensity of each band by the expression of β-actin, the value is shown with the parent strain as 1. The protein expressions of ALOX5 and 12 were down-regulated in CRR cells. However, ALOX15 was not down-regulated in protein level in SAS CRR cells.
Article Snippet:
Techniques: Expressing, Western Blot, Gene Expression