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p63 antibody  (Novus Biologicals)
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Novus Biologicals p63 antibody
Figure 6. Response to dactolisib is dependent upon TP53 mutation status. (A) Western blotting on lysates from NCI-H358 and NCI-H1299 cells following a cisplatin pulse, with the addition of dactolisib (1 mM), as indicated. (B) Quantification of effects of dactolisib upon cisplatin induced <t>p63</t> and p21 expression (n = 3, mean ± SD). (C) Western blotting on lysates from NCI-H358 cells, treated with P70S6K or control siRNA, as indicated, prior to and following a cisplatin pulse (n = 3, mean ± SD). (D) Apoptosis measured by propidium iodide staining for the sub-G1 population performed 72 hr following a cisplatin pulse with the addition of dactolisib (1 mM) as indicated (n = 3, mean ± SD). (E) Western blotting on lysates from A549 and NCI- H1573 cells, 48 hr following a cisplatin pulse, with the addition of dactolisib (1 mM), as indicated (n = 3, mean ± SD). (F) Western blotting across a second panel of lung adenocarcinoma cell lines. (G) Correlation between P70S6K phosphorylation and apoptosis, as measured by propidium iodide staining for the sub-G1 population, performed 72 hr following a cisplatin pulse (n = 3, mean ± SD). For all panels the statistical significance was determined by one-way ANOVA (***p<0.001, **p<0.01, *p<0.05).
P63 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nb100-56549/p63%2FTP73L+Antibody+(87C553)/10__7554_slash_elife__53367-227-1-6
Average 90 stars, based on 1 article reviews
p63 antibody - by Bioz Stars, 2026-10
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Figure 6. Response to dactolisib is dependent upon TP53 mutation status. (A) Western blotting on lysates from NCI-H358 and NCI-H1299 cells following a cisplatin pulse, with the addition of dactolisib (1 mM), as indicated. (B) Quantification of effects of dactolisib upon cisplatin induced p63 and p21 expression (n = 3, mean ± SD). (C) Western blotting on lysates from NCI-H358 cells, treated with P70S6K or control siRNA, as indicated, prior to and following a cisplatin pulse (n = 3, mean ± SD). (D) Apoptosis measured by propidium iodide staining for the sub-G1 population performed 72 hr following a cisplatin pulse with the addition of dactolisib (1 mM) as indicated (n = 3, mean ± SD). (E) Western blotting on lysates from A549 and NCI- H1573 cells, 48 hr following a cisplatin pulse, with the addition of dactolisib (1 mM), as indicated (n = 3, mean ± SD). (F) Western blotting across a second panel of lung adenocarcinoma cell lines. (G) Correlation between P70S6K phosphorylation and apoptosis, as measured by propidium iodide staining for the sub-G1 population, performed 72 hr following a cisplatin pulse (n = 3, mean ± SD). For all panels the statistical significance was determined by one-way ANOVA (***p<0.001, **p<0.01, *p<0.05).

Journal: eLife

Article Title: Analysis of pulsed cisplatin signalling dynamics identifies effectors of resistance in lung adenocarcinoma

doi: 10.7554/elife.53367

Figure Lengend Snippet: Figure 6. Response to dactolisib is dependent upon TP53 mutation status. (A) Western blotting on lysates from NCI-H358 and NCI-H1299 cells following a cisplatin pulse, with the addition of dactolisib (1 mM), as indicated. (B) Quantification of effects of dactolisib upon cisplatin induced p63 and p21 expression (n = 3, mean ± SD). (C) Western blotting on lysates from NCI-H358 cells, treated with P70S6K or control siRNA, as indicated, prior to and following a cisplatin pulse (n = 3, mean ± SD). (D) Apoptosis measured by propidium iodide staining for the sub-G1 population performed 72 hr following a cisplatin pulse with the addition of dactolisib (1 mM) as indicated (n = 3, mean ± SD). (E) Western blotting on lysates from A549 and NCI- H1573 cells, 48 hr following a cisplatin pulse, with the addition of dactolisib (1 mM), as indicated (n = 3, mean ± SD). (F) Western blotting across a second panel of lung adenocarcinoma cell lines. (G) Correlation between P70S6K phosphorylation and apoptosis, as measured by propidium iodide staining for the sub-G1 population, performed 72 hr following a cisplatin pulse (n = 3, mean ± SD). For all panels the statistical significance was determined by one-way ANOVA (***p<0.001, **p<0.01, *p<0.05).

Article Snippet: The p63 antibody (NB100-691) was from Novus Biologicals (CO, USA).

Techniques: Mutagenesis, Western Blot, Expressing, Control, Staining, Phospho-proteomics