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Novus Biologicals 5 ht7r
Fig. 5. Concentration-dependent effects of subchronic administration of lur asidone on <t>5-HT7R</t> protein expression in the plasma membrane fraction, using capillary immunoblotting. In the right-side panels pseudo-gel images of GAPDH and 5-HT7R using capillary immunoblotting were represented. In the left-side panels, ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT7R (per GAPDH). Concentration-dependent effects of lurasidone (100 and 300 nM) on 5-HT7R expression in the primary cultured astrocytes were ana lysed by one-way ANOVA with Tukey’s post hoc test (*P < 0.05, **P < 0.01 vs. control (lurasidone free)).
5 Ht7r, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 5. Concentration-dependent effects of subchronic administration of lur asidone on 5-HT7R protein expression in the plasma membrane fraction, using capillary immunoblotting. In the right-side panels pseudo-gel images of GAPDH and 5-HT7R using capillary immunoblotting were represented. In the left-side panels, ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT7R (per GAPDH). Concentration-dependent effects of lurasidone (100 and 300 nM) on 5-HT7R expression in the primary cultured astrocytes were ana lysed by one-way ANOVA with Tukey’s post hoc test (*P < 0.05, **P < 0.01 vs. control (lurasidone free)).

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Impact of 5-HT7 receptor inverse agonism of lurasidone on monoaminergic tripartite synaptic transmission and pathophysiology of lower risk of weight gain.

doi: 10.1016/j.biopha.2022.112750

Figure Lengend Snippet: Fig. 5. Concentration-dependent effects of subchronic administration of lur asidone on 5-HT7R protein expression in the plasma membrane fraction, using capillary immunoblotting. In the right-side panels pseudo-gel images of GAPDH and 5-HT7R using capillary immunoblotting were represented. In the left-side panels, ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT7R (per GAPDH). Concentration-dependent effects of lurasidone (100 and 300 nM) on 5-HT7R expression in the primary cultured astrocytes were ana lysed by one-way ANOVA with Tukey’s post hoc test (*P < 0.05, **P < 0.01 vs. control (lurasidone free)).

Article Snippet: Antibodies against GAPDH (NB300–327, 1:300, Novus Biologicals, Littleton, CO, USA), connexin43 (C6219, 1:100, Sigma-Aldrich, St. Louis, MO, USA), 5-HT1AR (NBP2–21590, 1:00, Novus Biologicals), 5-HT7R (NB100–56352, 1:00, Novus Biologicals), ERK (AF1576, 10 μg/mL, R&D systems, Minneapolis, MN, USA), phosphorylated-ERK (AF1018, 5 μg/mL, R&D Systems), AKT (AF1775, 1 μg/mL, R&D Systems), phosphorylated-AKT (AF877, 5 μg/mL, R&D Systems), AMPKα (2603, 1:50, Cell Signalling Technology, Danvers, MA, USA), and phosphorylated-AMPKα (2535, 1:50, Cell Signalling Technology) were diluted in an antibody diluent (Immuno Shot Platinum, CosmoBio) [23].

Techniques: Concentration Assay, Expressing, Clinical Proteomics, Membrane, Western Blot, Cell Culture, Control