nb100-56145 Search Results


livin α  (Novus Biologicals)
93
Novus Biologicals livin α
FIGURE 1 Identification of rAd‐infected DCs in vitro. (A–C) rAd‐FAP, rAd‐hlivin α, and rAd‐FAP/hlivin α were constructed using mouse FAP cDNA and human <t>livin</t> <t>α</t> cDNA, and then transduced into mouse bone marrow‐derived DCs, respectively. For evaluation of transduction efficacy, western blot was performed to measure protein expressions of FAP and livin α in cells, with GAPDH functioned as a loading control. ***p < .001, versus 1#; +++p < .001, versus 2#; ^^^p < .001, versus 3#. DCs, dendritic cells; FAP, fibroblast activation protein‐α; rAd‐FAP, recombinant adenoviral vector encoding mouse FAP cDNA; rAd‐hlivin α, recombinant adenoviral vector encoding human livin α cDNA.
Livin α, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nb100-56145/pm37773704-71-25-30?v=Novus+Biologicals
Average 93 stars, based on 1 article reviews
livin α - by Bioz Stars, 2026-07
93/100 stars
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FIGURE 1 Identification of rAd‐infected DCs in vitro. (A–C) rAd‐FAP, rAd‐hlivin α, and rAd‐FAP/hlivin α were constructed using mouse FAP cDNA and human livin α cDNA, and then transduced into mouse bone marrow‐derived DCs, respectively. For evaluation of transduction efficacy, western blot was performed to measure protein expressions of FAP and livin α in cells, with GAPDH functioned as a loading control. ***p < .001, versus 1#; +++p < .001, versus 2#; ^^^p < .001, versus 3#. DCs, dendritic cells; FAP, fibroblast activation protein‐α; rAd‐FAP, recombinant adenoviral vector encoding mouse FAP cDNA; rAd‐hlivin α, recombinant adenoviral vector encoding human livin α cDNA.

Journal: Immunity, inflammation and disease

Article Title: Dendritic cells infected with recombinant adenoviral vector encoding mouse fibroblast activation protein-α and human livin α exert an antitumor effect against Lewis lung carcinoma in mice.

doi: 10.1002/iid3.1011

Figure Lengend Snippet: FIGURE 1 Identification of rAd‐infected DCs in vitro. (A–C) rAd‐FAP, rAd‐hlivin α, and rAd‐FAP/hlivin α were constructed using mouse FAP cDNA and human livin α cDNA, and then transduced into mouse bone marrow‐derived DCs, respectively. For evaluation of transduction efficacy, western blot was performed to measure protein expressions of FAP and livin α in cells, with GAPDH functioned as a loading control. ***p < .001, versus 1#; +++p < .001, versus 2#; ^^^p < .001, versus 3#. DCs, dendritic cells; FAP, fibroblast activation protein‐α; rAd‐FAP, recombinant adenoviral vector encoding mouse FAP cDNA; rAd‐hlivin α, recombinant adenoviral vector encoding human livin α cDNA.

Article Snippet: Following incubation with blocking buffer (P0231, Beyotime), Western blot was performed by incubating the membranes with primary antibodies against FAP (NB110‐85534, 88 kDa, Novus Biologicals), livin α (NB100‐56145, 33 kDa, Novus Biologicals), CD45 (ab10558, 147 kDa, Abcam), CD31 (ab281583, 82 kDa, Abcam), α‐SMA (ab5694, 42 kDa, Abcam), and internal control GAPDH (ab8245, 37 kDa, Abcam) at 4°C overnight.

Techniques: Infection, In Vitro, Construct, Derivative Assay, Transduction, Western Blot, Control, Activation Assay, Recombinant, Plasmid Preparation

FIGURE 2 Identification of LLC tumor‐isolated CAFs and determination on expressions of FAP and livin α in LLC. (A–C) Western blot was performed to measure protein expressions of CD45, CD31, α‐SMA, and FAP in CAFs isolated from LLC‐bearing mice as well as the protein expression of livin α in mouse LLC tumors. GAPDH was used as the loading control. +++p < .001, versus lysate; ^^^p < .001, versus tumor; ##p < .001, versus Para. CAFs, cancer‐associated fibroblasts; LLC, Lewis lung carcinoma; Para, paracancerous tissues.

Journal: Immunity, inflammation and disease

Article Title: Dendritic cells infected with recombinant adenoviral vector encoding mouse fibroblast activation protein-α and human livin α exert an antitumor effect against Lewis lung carcinoma in mice.

doi: 10.1002/iid3.1011

Figure Lengend Snippet: FIGURE 2 Identification of LLC tumor‐isolated CAFs and determination on expressions of FAP and livin α in LLC. (A–C) Western blot was performed to measure protein expressions of CD45, CD31, α‐SMA, and FAP in CAFs isolated from LLC‐bearing mice as well as the protein expression of livin α in mouse LLC tumors. GAPDH was used as the loading control. +++p < .001, versus lysate; ^^^p < .001, versus tumor; ##p < .001, versus Para. CAFs, cancer‐associated fibroblasts; LLC, Lewis lung carcinoma; Para, paracancerous tissues.

Article Snippet: Following incubation with blocking buffer (P0231, Beyotime), Western blot was performed by incubating the membranes with primary antibodies against FAP (NB110‐85534, 88 kDa, Novus Biologicals), livin α (NB100‐56145, 33 kDa, Novus Biologicals), CD45 (ab10558, 147 kDa, Abcam), CD31 (ab281583, 82 kDa, Abcam), α‐SMA (ab5694, 42 kDa, Abcam), and internal control GAPDH (ab8245, 37 kDa, Abcam) at 4°C overnight.

Techniques: Isolation, Western Blot, Expressing, Control

FIGURE 3 The antitumor effect of rAd‐FAP/hlivin α‐transduced DCs on LLC in mice. (A and B) Mice were separately immunized with rAd‐EGFP‐transduced DCs, rAd‐FAP‐transduced DCs, rAd‐hlivin α‐transduced DCs, and rAd‐FAP/hlivin α‐transduced DCs (5 × 105 cells/ mouse) on Day 8 postinjection with LLC cells, and tumor volume (mm3) was calculated every 2 days for a total 24 days. (C) Mouse survival rate was observed for 80 days. (D) Splenic lymphocytes were obtained from mice on Day 7 after receiving injection with rAd‐EGFP‐ transduced DCs, rAd‐FAP‐transduced DCs, rAd‐hlivin α‐transduced DCs or rAd‐FAP/hlivin α‐transduced DCs. The cytotoxic effect of splenic lymphocytes on LLC‐derived CAFs was analyzed using Cytotox96 Non‐Radioactive Cytotoxicity Assay Kit. ***p < .001, versus 1#; +p < .05, +++p < .001, versus 2#; ^p < .05, ^^p < .01, ^^^p < .001, versus 3#. CAFs, cancer‐associated fibroblasts; DCs, dendritic cells; LLC, Lewis lung carcinoma; FAP, fibroblast activation protein‐α; rAd‐FAP, recombinant adenoviral vector encoding mouse FAP cDNA; rAd‐hlivin α, recombinant adenoviral vector encoding human livin α cDNA.

Journal: Immunity, inflammation and disease

Article Title: Dendritic cells infected with recombinant adenoviral vector encoding mouse fibroblast activation protein-α and human livin α exert an antitumor effect against Lewis lung carcinoma in mice.

doi: 10.1002/iid3.1011

Figure Lengend Snippet: FIGURE 3 The antitumor effect of rAd‐FAP/hlivin α‐transduced DCs on LLC in mice. (A and B) Mice were separately immunized with rAd‐EGFP‐transduced DCs, rAd‐FAP‐transduced DCs, rAd‐hlivin α‐transduced DCs, and rAd‐FAP/hlivin α‐transduced DCs (5 × 105 cells/ mouse) on Day 8 postinjection with LLC cells, and tumor volume (mm3) was calculated every 2 days for a total 24 days. (C) Mouse survival rate was observed for 80 days. (D) Splenic lymphocytes were obtained from mice on Day 7 after receiving injection with rAd‐EGFP‐ transduced DCs, rAd‐FAP‐transduced DCs, rAd‐hlivin α‐transduced DCs or rAd‐FAP/hlivin α‐transduced DCs. The cytotoxic effect of splenic lymphocytes on LLC‐derived CAFs was analyzed using Cytotox96 Non‐Radioactive Cytotoxicity Assay Kit. ***p < .001, versus 1#; +p < .05, +++p < .001, versus 2#; ^p < .05, ^^p < .01, ^^^p < .001, versus 3#. CAFs, cancer‐associated fibroblasts; DCs, dendritic cells; LLC, Lewis lung carcinoma; FAP, fibroblast activation protein‐α; rAd‐FAP, recombinant adenoviral vector encoding mouse FAP cDNA; rAd‐hlivin α, recombinant adenoviral vector encoding human livin α cDNA.

Article Snippet: Following incubation with blocking buffer (P0231, Beyotime), Western blot was performed by incubating the membranes with primary antibodies against FAP (NB110‐85534, 88 kDa, Novus Biologicals), livin α (NB100‐56145, 33 kDa, Novus Biologicals), CD45 (ab10558, 147 kDa, Abcam), CD31 (ab281583, 82 kDa, Abcam), α‐SMA (ab5694, 42 kDa, Abcam), and internal control GAPDH (ab8245, 37 kDa, Abcam) at 4°C overnight.

Techniques: Injection, Derivative Assay, Cytotoxicity Assay, Activation Assay, Recombinant, Plasmid Preparation